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Invitrogen
CD1d Monoclonal Antibody (WTH-2), APC, eBioscience™
FIGURE: 1 / 3
CD1d Antibody (17-0013-80) in Flow
Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.125 µg of Mouse IgG2a K Isotype Control APC (Product # 17-4724-81) (left) or 0.125 µg of Anti-Mouse/Rat CD1d APC (right). Total viable cells were used for analysis.
Product Details
17-0013-80
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Mouse
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
WTH-2
Conjugate
Excitation/Emission Max
651/660 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573136
Product Specific Information
Description: The monoclonal antibody WTH-2 reacts with mouse and rat CD1d. CD1d is a non-polymorphic MHC Class I-like molecule composed of alpha-1 and alpha-2 domains. CD1d is constitutively expressed on hematopoietic cells, with highest levels on dendritic cells, macrophages, and marginal zone B-cells. It is also expressed on non-lymphatic organs such as the liver, heart, small intestine, and pancreas. CD1d associates with beta2-microglobulin to present antigens containing a lipid or other hydrophobic moieties to invariant NKT cells, which leads to rapid Th1 or Th2 cytokine secretion.
The WTH-2 antibody has been reported to have antagonist activity.
Applications Reported: This WTH-2 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This WTH-2 antibody has been tested by flow cytometric analysis of mouse or rat splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD1d is a heavy chain associated with Beta 2 microglobulin on cortical thymocytes. Beta 2 microglobulin independent expression of CD1d has also been demonstrated on human intestinal epithelial cells. CD1d is the sole group 2 member of the CD1 family of major histocompatibility (MHC) like glycoproteins. The CD1d gene encodes a divergent member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. The CD1 proteins mediate the presentation of primarily lipid and glycolipid antigens of self or microbial origin to T cells. The human genome contains five CD1 family genes organized in a cluster on chromosome 1. The CD1 family members are thought to differ in their cellular localization and specificity for particular lipid ligands. The protein encoded by the CD1d gene localizes to late endosomes and lysosomes via a tyrosine-based motif in the cytoplasmic tail. Diseases associated with CD1D include Mycobacterium Malmoense and Autoimmune Disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
How to use the Panel Builder
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
Bioinformatics
Protein Aliases: Antigen-presenting glycoprotein CD1d; Antigen-presenting glycoprotein CD1d1; CD1d; CD1D antigen; CD1d.1; CD1d1 antigen; T-cell surface glycoprotein CD1d; T-cell surface glycoprotein CD1d1
Gene Aliases: AI747460; Cd1; CD1.1; Cd1a; Cd1d; Cd1d1; Ly-38
UniProt ID: (Mouse) P11609, (Rat) Q63493
Entrez Gene ID: (Mouse) 12479, (Rat) 25109
positive regulation of T cell mediated cytotoxicity
immune system process
immune response
antigen processing and presentation
positive regulation of T cell proliferation
positive regulation of macrophage activation
positive thymic T cell selection
positive regulation of interferon-gamma biosynthetic process
positive regulation of interleukin-2 biosynthetic process
innate immune response
positive regulation of interleukin-4 biosynthetic process
antigen processing and presentation, endogenous lipid antigen via MHC class Ib
antigen processing and presentation, exogenous lipid antigen via MHC class Ib
regulation of immune response
positive regulation of NK T cell activation
positive regulation of NK T cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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