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Invitrogen
CD200 Receptor Monoclonal Antibody (OX108), PerCP-eFluor™ 710, eBioscience™
FIGURE: 1 / 2
CD200 Receptor Antibody (46-9201-42) in Flow
Staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0047-42) and Mouse IgG1 K Isotype Control PerCP-eFluor® 710 (Product # 46-4714-82) (left) or Anti-Human CD200 Receptor PerCP-eFluor® 710 (right). Cells in the lymphocyte gate were used for analysis.
Product Details
46-9201-42
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Not Applicable
Host/Isotype
Mouse
/ IgG1, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
OX108
Conjugate
Excitation/Emission Max
482/708 nm
View spectra
Form
Liquid
Concentration
5 µL/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2573879
Product Specific Information
Description: The monoclonal antibody OX108 recognizes human CD200R also known as OX2. CD200R is an inhibitory receptor with a similar structure as its ligand, CD200, yet lacks an ITIM domain typically found in inhibitory receptors. Instead the CD200R cytoplasmic domain contains a novel phosphotyrosine binding domain, NPXY, which after binding SHIP inhibits ERK, JNK and MAPK p38 pathways. In activated macrophages signaling results in inhibition of TNFalpha secretion. Isoforms of CD200R have been identified and are thought to play a major role in differentiation, especially in regards to tolerogenic DCs.
Expression is restricted to hematopoietic cells: myeloid cells (monocytes, macrophages, DCs, neutrophils, mast cells and basophils) and a subset of T lymphocytes as well as langerhans (LC) cells and dendritic epidermal T cells. The epitope of OX108 is thought to be near the binding site of CD200.
Applications Reported: This OX108 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This OX108 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm); it can be used in place of PerCP-Cyanine5.5. We recommend using a 710/50 bandpass filter, however, the 695/40 bandpass filter is an acceptable alternative. Please make sure that your instrument is capable of detecting this fluorochrome.
Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.
Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
CD200R (OX2R) is a receptor for the OX-2 membrane glycoprotein, and both the receptor and substrate are cell surface glycoproteins containing two immunoglobulin-like domains. CD200R is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid downregulatory signal. In mouse, CD200R is expressed primarily by peripheral blood monocytes and neutophils but also by other leucocytes including T-lymphocytes and mast cells. Further mouse studies of a gene related to CD200R suggests that the receptor may control myeloid function in a tissue-specific manner. Alternative splicing of CD200R results in multiple transcript variants. Diseases associated with CD200R dysfunction include primary hypertrophic osteoarthropathy.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD200 cell surface glycoprotein receptor; CD200 R1; Cell surface glycoprotein CD200 receptor 1; Cell surface glycoprotein OX2 receptor 1; cell surface glycoprotein receptor CD200; MOX2 receptor
Gene Aliases: CD200R; CD200R1; CRTR2; HCRTR2; MOX2R; OX2R; UNQ2522/PRO6015
UniProt ID: (Human) Q8TD46
Entrez Gene ID: (Human) 131450
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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