Invitrogen

CD206 Monoclonal Antibody (MR5D3), FITC

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CD206 Antibody (MA5-16870) in Flow

Cancer cell international 2022 - Fig. 2 ADMA Drives Macrophages Towards an M2-like subtype. RAW264.7 cells were treat-ed with DPBS (control) or ADMA (5 mM) for 3 days. RNA was collected and expression of M1- and M2-related genes was analyzed. Many M2 related markers were strikingly induced, in-cluding ARG ( A ), CD163 ( B ) and CD206 ( C ) while the M1 related markers NOS ( D ) and TNFalpha ( E ) were slightly or not impacted by ADMA. Howe... View More

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Product Details

MA5-16870

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Neat

View 12 publications 12 publications

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a

Class

Monoclonal

Type

Antibody

Clone

MR5D3

Immunogen

Chimaeric CRD4-7-Fc protein.

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.1 mg/mL

Purification

Protein G

Storage buffer

PBS with 1% BSA

Contains

0.09% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. Store in the dark.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2538348

Product Specific Information

CD206 is expressed weakly at the cell surface. Staining may be increased following membrane permeabilization. For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.

Rat anti mouse CD206 antibody, clone MR5D3 recognizes the mouse mannose receptor, a approximately 175 kDa type 1 membrane glycoprotein that is also known as CD206.

Target Information

CD206 (MSR, Mannose receptor, MRC1) is a 175 kDa transmembrane protein belonging to the group of pattern recognition receptors. CD206 is predominantly expressed in tissue macrophages, dendritic cells, a subpopulation of endothelial cells and sperm cells. CD206 is thought to play a role in the innate and adaptive immune response. CD206 is also expressed on microglia and mato cells of the brain but not astrocytes or neurons. CD206 also mediate the recognition and uptake of a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid b-protein (Abeta). While the normal role of CD206 is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and Amyloid beta deposition in Alzheimer's disease (AD). CD206’s gene encodes the class A macrophage scavenger receptors, which include three different types (1, 2, 3) generated by alternative splicing. The isoforms type 1 and type 2 are functional receptors and are able to mediate the endocytosis of modified low density lipoproteins (LDLs). The isoform type 3 does not internalize modified LDL (acetyl-LDL) despite having the domain shown to mediate this function in the types 1 and 2 isoforms. CD206 has an altered intracellular processing and is trapped within the endoplasmic reticulum, making it unable to perform endocytosis. The isoform type 3 can inhibit the function of isoforms type 1 and type 2 when co-expressed, indicating a dominant negative effect and suggesting a mechanism for regulation of scavenger receptor activity in macrophages. Other diseases associated with CD206 dysfunction include leprosy and Gaucher’s Disease.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Oral Exposure to Polystyrene Microplastics of Mice on a Normal or High-Fat Diet and Intestinal and Metabolic Outcomes.

Environmental health perspectives

Okamura T,Hamaguchi M,Hasegawa Y,Hashimoto Y,Majima S,Senmaru T,Ushigome E,Nakanishi N,Asano M,Yamazaki M,Sasano R,Nakanishi Y,Seno H,Takano H,Fukui M

MA5-16870 was used in Flow cytometry/Cell sorting to highlight the need for reducing oral exposure to MPs through remedial environmental measures to improve metabolic disturbance under high-fat diet conditions.

Wed Feb 01 00:00:00 EST 2023

Single-cell sequencing reveals homogeneity and heterogeneity of the cytopathological mechanisms in different etiology-induced AKI.

Cell death & disease

Chen Z,Li Y,Yuan Y,Lai K,Ye K,Lin Y,Lan R,Chen H,Xu Y

MA5-16870 was used in Flow Cytometry to provide a potent resource for understanding the pathogenesis of acute kidney injury AKI and early intervention in AKI progression at single-cell resolution.

Thu May 11 00:00:00 EDT 2023

Tumor cell-derived asymmetric dimethylarginine regulates macrophage functions and polarization.

Cancer cell international

Chen YL,Lowery AT,Lin S,Walker AM,Chen KE

Published figure using CD206 monoclonal antibody (Product # MA5-16870) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

The alveolar macrophage toponome of female SP-A knockout mice differs from that of males before and after SP-A1 rescue.

Scientific reports

Phelps DS,Chinchilli VM,Yang L,Shearer D,Weisz J,Zhang X,Floros J

MA5-16870 was used in flow cytometry to study the expression of alveolar macrophages in male and female mice lacking surfactant protein A.

Wed Mar 23 00:00:00 EDT 2022

The Effect of miR-520b on Macrophage Polarization and T Cell Immunity by Targeting PTEN in Breast Cancer.

Journal of oncology

Zhu Q,Yuan J,He Y,Hu Y

MA5-16870 was used in Flow cytometry/Cell sorting to explore the effect of miR-520b and PTEN on breast cancer and the mechanisms involved.

Wed Apr 27 00:00:00 EDT 2022

The Effect of miR-520b on Macrophage Polarization and T Cell Immunity by Targeting PTEN in Breast Cancer.

Journal of oncology

Zhu Q,Yuan J,He Y,Hu Y

MA5-16870 was used in Flow cytometry/Cell sorting to explore the effect of miR-520b and PTEN on breast cancer and the mechanisms involved.

Wed Apr 27 00:00:00 EDT 2022

Tilapia Piscidin 4 (TP4) Reprograms M1 Macrophages to M2 Phenotypes in Cell Models of Gardnerella vaginalis-Induced Vaginosis.

Frontiers in immunology

Liu CW,Su BC,Chen JY

MA5-16870 was used in Flow cytometry/Cell sorting to show that TP4 may be able to attenuate the virulence of G. vaginalis by inducing resolving M2c and tissue repair M2a macrophage polarizations, suggesting a novel strategy for BV therapy.

Wed Feb 16 00:00:00 EST 2022

Tilapia Piscidin 4 (TP4) Reprograms M1 Macrophages to M2 Phenotypes in Cell Models of Gardnerella vaginalis-Induced Vaginosis.

Frontiers in immunology

Liu CW,Su BC,Chen JY

Wed Feb 16 00:00:00 EST 2022

Scientific reports

Harada T,Tsuboi I,Hino H,Yuda M,Hirabayashi Y,Hirai S,Aizawa S

MA5-16870 was used in Flow cytometry/Cell sorting to find that the dosing of SAMP1/TA-1 mice with LPS revealed that the suppression of myelopoiesis and B-lymphopoiesis was more severe in aged mice than in young mice.

Wed Dec 01 00:00:00 EST 2021

Scientific reports

Harada T,Tsuboi I,Hino H,Yuda M,Hirabayashi Y,Hirai S,Aizawa S

Wed Dec 01 00:00:00 EST 2021

Topical arginase inhibition decreases growth of cutaneous squamous cell carcinoma.

Scientific reports

Mittal A,Wang M,Vidyarthi A,Yanez D,Pizzurro G,Thakral D,Tracy E,Colegio OR

MA5-16870 was used in Flow Cytometry to demonstrate the therapeutic potential of transcutaneous arginase inhibition in cutaneous squamous cell carcinomas (cSCC).

Mon May 24 00:00:00 EDT 2021

Ribonuclease T2 from Aspergillus fumigatus promotes T helper type 2 responses through M2 polarization of macrophages.

International journal of molecular medicine

Chen JJ,He YS,Zhong XJ,Cai ZL,Lyu YS,Zhao ZF,Ji K

MA5-16870 was used in Flow cytometry/Cell sorting to reveal that high rAf RNASET2 activity is required for rAf RNASET2‚induced M2 polarization of macrophages.

Sat Aug 01 00:00:00 EDT 2020

Ribonuclease T2 from Aspergillus fumigatus promotes T helper type 2 responses through M2 polarization of macrophages.

International journal of molecular medicine

Chen JJ,He YS,Zhong XJ,Cai ZL,Lyu YS,Zhao ZF,Ji K

MA5-16870 was used in Flow cytometry/Cell sorting to reveal that high rAf RNASET2 activity is required for rAf RNASET2‚induced M2 polarization of macrophages.

Sat Aug 01 00:00:00 EDT 2020

Immune modulating effects of additional supplementation of estradiol combined with testosterone in murine testosterone-deficient NAFLD model.

American journal of physiology. Gastrointestinal and liver physiology

Okamura T,Hamaguchi M,Bamba R,Nakajima H,Yoshimura Y,Kimura T,Nishida K,Hashimoto Y,Fukuda T,Senmaru T,Fukui M

MA5-16870 was used in Flow cytometry/Cell sorting to suggest that a combination of estrogen and testosterone may be clinically relevant for the treatment of NAFLD in patients with male menopause.

Mon Jun 01 00:00:00 EDT 2020

Biochanin a Enhances the Defense Against Salmonella enterica Infection Through AMPK/ULK1/mTOR-Mediated Autophagy and Extracellular Traps and Reversing SPI-1-Dependent Macrophage (MΦ) M2 Polarization.

Frontiers in cellular and infection microbiology

Zhao X,Tang X,Guo N,An Y,Chen X,Shi C,Wang C,Li Y,Li S,Xu H,Liu M,Wang Y,Yu L

Published figure using CD206 monoclonal antibody (Product # MA5-16870) in Flow Cytometry

Fri Aug 30 00:00:00 EDT 2019

RETRACTED: Modulation of macrophage phenotype through controlled release of interleukin-4 from gelatine coatings on titanium surfaces.

European cells & materials

Yang CL,Sun YH,Yu WH,Yin XZ,Weng J,Feng B

MA5-16870 was used in Flow cytometry/Cell sorting to assess the effectiveness of biomaterials in the modulation of inflammation. In this study, a hydrogel coating on a titanium surface temporally released IL-4, enabling a timed switch from M1 to M2 macrophage phenotype in a simulated inflammatory environment.

Wed Jul 25 00:00:00 EDT 2018

miR-142-5p and miR-130a-3p are regulated by IL-4 and IL-13 and control profibrogenic macrophage program.

Nature communications

Su S,Zhao Q,He C,Huang D,Liu J,Chen F,Chen J,Liao JY,Cui X,Zeng Y,Yao H,Su F,Liu Q,Jiang S,Song E

MA5-16870 was used in flow cytometry to investigate if microRNAs contribute to macrophage's fibrogenesis

Mon Oct 05 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD206; Macrophage mannose receptor 1; MMR

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Gene Aliases: AW259686; CD206; MR; Mrc1

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UniProt ID: (Mouse) Q61830

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Entrez Gene ID: (Mouse) 17533

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Function(s)

receptor activity transmembrane signaling receptor activity mannose binding carbohydrate binding

Process(es)

endocytosis receptor-mediated endocytosis signal transduction cellular response to lipopolysaccharide cellular response to interferon-gamma cellular response to interleukin-4

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD206 Monoclonal Antibody (MR5D3), FITC

CD206 Antibody (MA5-16870) in Flow

Product Details

MA5-16870

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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