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            • Primary Antibodies ›
            • CD235a (Glycophorin A) Antibodies

            Invitrogen

            CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PE, eBioscience™

            32 Published Figures
            43 References
            View all (98) CD235a (Glycophorin A) antibodies

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            Datasheet
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            Catalog # 12-9987-82
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            Cite CD235a (Glycophorin A) Monoclonal Antibody (HIR2 (GA-R2)), PE, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (32)
            Application
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 33

            CD235a (Glycophorin A) Antibody (12-9987-82) in Flow

            Staining of normal human peripheral blood cells with Mouse IgG2b kappa Isotype Control PE (Product # 12-4732-81) (blue histogram) or Anti-Human CD235a (Glycophorin A) PE (purple histogram). Non-leukocytes were used for analysis.
            View Product

            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)
            CD235a (Glycophorin A) Antibody in Flow Cytometry (Flow)

            Product Details

            12-9987-82

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            0.015 µg/test
            View 43 publications 43 publications
            Product Specifications

            Species Reactivity

            Human

            Published species

            Human, Mouse

            Host/Isotype

            Mouse / IgG2b, kappa

            Recommended Isotype Control

            Mouse IgG2b kappa Isotype Control (eBMG2b), PE, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            HIR2 (GA-R2)

            Conjugate

            PE
            • Unconjugated
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 661
            • eFluor 450
            • FITC
            • NovaFluor Blue 510
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Red 685
            • NovaFluor Red 700
            • NovaFluor Yellow 610
            • NovaFluor Yellow 660
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Excitation/Emission Max

            565/576 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.2 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_466300

            Product Specific Information

            Description: The HIR2 monoclonal antibody reacts with human glycophorin A, sialoglycoproteins expressed by erythroid precursors and mature circulating red cells.

            Applications Reported: The HIR2 (also GA-R2) antibody has been reported for use in flow cytometric analysis.

            Applications Tested: The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.015 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            Glycophorin A, also known as CD235a, is a 151 amino acid sialoglycoprotein expressed on the membrane of mature erythrocytes and erythroid progenitor cells, with approximately 500,000 copies per cell. The gene for glycophorin A is located on chromosome 4 and exists in two allelic forms, M and N, which differ by two amino acids: the M group has Ser1 and Gly5, while the N group has Leu1 and Glu5. These allelic variations define the blood group M and N specificities. Glycophorin A serves multiple functions, including providing a mucin-like barrier that minimizes aggregation between red blood cells in circulation, potentially preventing cell fusion. It also acts as a receptor for certain pathogens, including Sandei virus, parvovirus, and Hsa, a Streptococcus adhesin. Recent studies suggest that exposure to toxins can lead to mutations or loss of alleles, resulting in phenotypic changes. There is ongoing research correlating genotype and phenotype with predisposition to diseases such as cancer and heart disease, highlighting the importance of glycophorin A in both health and disease. Glycophorin A is a significant marker for studying erythrocyte-related functions and pathologies, as well as its role in pathogen interactions and disease predisposition.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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              Bioinformatics

              Protein Aliases: CD235; CD235a; erythroid-lineage-specific membrane sialoglycoprotein; glycophorin A (MN blood group); glycophorin A, GPA; glycophorin Erik; glycophorin MiI; glycophorin MiV; glycophorin SAT; glycophorin Sta type C; Glycophorin-A; HGNC:4702; HGpMiX; HGpStaC; Mi.V glycoprotein; Mi.V glycoprotein (24 AA); MN sialoglycoprotein; PAS-2; recombinant glycophorin A-B Miltenberger-DR; Sialoglycoprotein alpha

              View more View less

              Gene Aliases: CD235a; GPA; GPErik; GPSAT; GYPA; HGpMiV; HGpMiXI; HGpSta(C); MN; MNS; PAS-2

              View more View less

              UniProt ID: (Human) P02724

              View more View less

              Entrez Gene ID: (Human) 2993

              View more View less

              Function(s)

              virus receptor activity identical protein binding

              Process(es)

              viral entry into host cell
              It has to be done as per old AB suggested Products section.
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