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            • Primary Antibodies ›
            • CD266 Antibodies

            Invitrogen

            CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), Biotin, eBioscience™

            2 References
            View all (27) CD266 antibodies

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            Datasheet
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            Catalog # 13-9018-82
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            Cite CD266 (TWEAK Receptor) Monoclonal Antibody (ITEM-4), Biotin, eBioscience™

            • Antibody Testing Data (1)
            Application
            CD266 (TWEAK Receptor) Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 1

            CD266 (TWEAK Receptor) Antibody (13-9018-82) in Flow

            Staining of non-transfected (left) and human CD266 transfected (right) L5178Y cells with 0.25 µg of Mouse IgG2b Iso Control (Product # 13-4732-85) (open histogram) or 0.25 µg Anti-Human/Mouse CD266 (TWEAK Receptor) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were analyzed.
            View Product

            CD266 (TWEAK Receptor) Antibody in Flow Cytometry (Flow)

            Product Details

            13-9018-82

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            -
            View 1 publication 1 publication

            Flow Cytometry (Flow)

            0.5 µg/test
            -

            Functional Assay (Functional)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Mouse

            Host/Isotype

            Mouse / IgG2b, kappa

            Recommended Isotype Control

            Mouse IgG2b kappa Isotype Control (eBMG2b), Biotin, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            ITEM-4

            Conjugate

            Biotin
            • Unconjugated
            • Functional Grade
            • PE 
            • Request custom conjugation

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, do not freeze

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_466960

            Product Specific Information

            Description: The ITEM-4 antibody reacts with human TWEAK Receptor/Fn14 (fibroblast growth factor-inducible 14 kDa protein). Fn14 is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. Fn14 plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. TWEAK-induced cell death via Fn14 includes both apoptosis and necrosis and can be blocked by an anti-TWEAK antibody, CARL-1. Fn14 is expressed on HUVEC and in some cancer tissues but not on freshly isolated PBMCs. Fn14 mRNA expression has been identified during liver regeneration. It has been reported that ITEM-4 cross-reacts with mouse Fn14. In the mouse, Fn14 is reported to be constitutively expressed by Colon 26 cell line.

            The ITEM-4 antibody has been show to have blocking function.

            Applications Reported: The ITEM-4 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: The ITEM-4 antibody has been tested by flow cytometric analysis on human and mouse Fn14 transfected cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            TWEAK is a member of the TNF family and was first described as a weak inducer of apoptosis in demonstrate that this relatively small molecule is a fully transformed cell lines. Recently, a receptor for TWEAK was isolated by expression cloning from a HUVEC cell cDNA library. TweakR is a small protein belonging to TNF receptor super family. TweakR is capable of intiating a proliferative signal by binding to TRAF1, 2, and 3. TweakR mRNA is upregulated during tissue regeneration in the aorta and liver. It may also play a role in vascular cell migration and angiogenesis.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            Bioinformatics

            Protein Aliases: CD266; FGF-inducible 14; fibroblast growth factor regulated protein 2; Fibroblast growth factor-inducible immediate-early response protein 14; Fibroblast growth factor-regulated protein 2; Tumor necrosis factor receptor superfamily member 12A; tumor necrosis factor receptor superfamily, member 12A; Tweak-receptor; TweakR; type I transmembrane protein Fn14

            View more View less

            Gene Aliases: AI255180; C87282; CD266; Fgfrp2; FN14; HPIP; TNFRSF12A; TWEAK-R; TWEAKR

            View more View less

            UniProt ID: (Human) Q9NP84, (Mouse) Q9CR75

            View more View less

            Entrez Gene ID: (Human) 51330, (Mouse) 27279

            View more View less

            Function(s)

            protein binding transmembrane signal receptor

            Process(es)

            angiogenesis substrate-dependent cell migration, cell attachment to substrate tumor necrosis factor-mediated signaling pathway positive regulation of apoptotic process regulation of angiogenesis positive regulation of axon extension regulation of wound healing extrinsic apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway apoptotic process cell adhesion multicellular organism development cell differentiation
            It has to be done as per old AB suggested Products section.
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