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Product Details
MUB0365P
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
LG.3A10
Immunogen
Mouse CD27
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A/G
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Clone 33B12 and 117C9 detect overlapping epitopes on the Forssman glycolipid hapten (GalNA1- c3aG alNAcD1 -3GalaI-4GalB1-4GlcBl-lCer). Clone 33B12 reacts with the terminal sugar sequence GalNAcd-3GalNAc and is specific for Forssman antigen.
The antibody may be stored at 4 C. For prolonged storage prepare appropriate aliquots and store at or below -20 C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4 C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.
Target Information
CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7
Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55
UniProt ID: (Human) P26842, (Mouse) P41272
Entrez Gene ID: (Human) 939, (Mouse) 21940
inflammatory response
immune response
cell surface receptor signaling pathway
brain development
release of cytoplasmic sequestered NF-kappaB
extrinsic apoptotic signaling pathway via death domain receptors
immunoglobulin mediated immune response
response to lipopolysaccharide
tumor necrosis factor-mediated signaling pathway
regulation of cell proliferation
negative regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
positive regulation of MAPK cascade
response to ethanol
positive regulation of B cell differentiation
positive regulation of JNK cascade
negative regulation of T cell apoptotic process
extrinsic apoptotic signaling pathway
apoptotic process
B cell proliferation
positive regulation of interferon-gamma biosynthetic process
positive regulation of T cell differentiation
immunoglobulin secretion
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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