Invitrogen

CD27 Monoclonal Antibody (LG.7F9), APC, eBioscience™

FIGURE: 1 / 21

CD27 Antibody (17-0271-82) in Flow

Staining of C57BL/6 splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.03 µg of Armenian Hamster IgG Isotype Control APC (Product # 17-4888-82) (left) or 0.03 µg of Anti-CD27 APC (right). Total viable cells were used for analysis.

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Product Details

17-0271-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.06 µg/test

View 30 publications 30 publications

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Armenian hamster / IgG

Recommended Isotype Control

Armenian Hamster IgG Isotype Control (eBio299Arm), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

LG.7F9

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469370

Product Specific Information

Description: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4+ and CD8+ effector cells. LG.7F9 cross-reacts with human and rat CD27.

Applications Reported: The LG.7F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The LG.7F9 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD27 is a 50 kDa member of the tumor necrosis factor (TNF) receptor superfamily that includes CD40 and CD30. The TNF superfamily members are known for the regulation of cell proliferation and death. In contrast to the expression of other TNFR/TNF family members, expression of CD27 and its ligand CD70 is predominantly confined to lymphocytes. High expression levels of CD27 appear to be dependent on proper ligation of antigen receptors. CD70 expression requires additional co-stimulatory and/or pro-inflammatory signals. CD27 is expressed as a disulfide-linked homodimer on mature thymocytes, peripheral blood T cells and a subpopulation of B cells. Activation of T cells via TCR-CD3 complex results in upregulation of CD27 expression on the plasma membrane as well as in the release of its soluble 28-32 kDa form, sCD27, detected in the plasma, urine or spinal fluid. Soluble CD27 is an important prognostic marker of acute and chronic B cell malignancies. RgpA, a cystein proteinase, although activating T cells through the protease-activated receptors (PARs), degradates CD27 and counteracts T cell activation mediated by CD27 and its ligand CD70. CD27-binding protein (SIVA), a proapoptotic protein, can bind to this receptor and is thought to play an important role in the apoptosis induced by this receptor. Diseases associated with CD27 dysfunction include Lymphoproliferative Syndrome 2 and Autosomal Recessive Lymphoproliferative Syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

17-0271-82 was used in Flow cytometry/Cell sorting to investigate the role of Bach2 in natural killer (NK) cell development, revealing that Bach2 expression peaked in immature CD27+CD11b+ cells, and its deficiency led to increased proportions of terminally differentiated NK cells with enhanced granzyme and cytokine production, as well as improved NK cell-mediated control of tumor metastasis.

Mon Oct 03 00:00:00 EDT 2022

The transcription factor Bach2 negatively regulates murine natural killer cell maturation and function.

eLife

Li S,Bern MD,Miao B,Fan C,Xing X,Inoue T,Piersma SJ,Wang T,Colonna M,Kurosaki T,Yokoyama WM

Mon Oct 03 00:00:00 EDT 2022

The transcriptional repressor ID2 supports natural killer cell maturation by controlling TCF1 amplitude.

The Journal of experimental medicine

Li ZY,Morman RE,Hegermiller E,Sun M,Bartom ET,Maienschein-Cline M,Sigvardsson M,Kee BL

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Mon Jun 07 00:00:00 EDT 2021

Chronic T cell proliferation in brains after stroke could interfere with the efficacy of immunotherapies.

The Journal of experimental medicine

Heindl S,Ricci A,Carofiglio O,Zhou Q,Arzberger T,Lenart N,Franzmeier N,Hortobagyi T,Nelson PT,Stowe AM,Denes A,Edbauer D,Liesz A

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Mon Aug 02 00:00:00 EDT 2021

PI3Kδ coordinates transcriptional, chromatin, and metabolic changes to promote effector CD8⁺ T cells at the expense of central memory.

Cell reports

Cannons JL,Villarino AV,Kapnick SM,Preite S,Shih HY,Gomez-Rodriguez J,Kaul Z,Shibata H,Reilley JM,Huang B,Handon R,McBain IT,Gossa S,Wu T,Su HC,McGavern DB,O'Shea JJ,McGuire PJ,Uzel G,Schwartzberg PL

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Tue Oct 12 00:00:00 EDT 2021

Effect of Subcutaneous Anti-CD20 Antibody-Mediated B Cell Depletion on Susceptibility to Pneumocystis Infection in Mice.

mSphere

Dai G,Noell K,Weckbecker G,Kolls JK

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Wed May 05 00:00:00 EDT 2021

Immune cell phenotypes associated with disease severity and long-term neutralizing antibody titers after natural dengue virus infection.

Cell reports. Medicine

Rouers A,Chng MHY,Lee B,Rajapakse MP,Kaur K,Toh YX,Sathiakumar D,Loy T,Thein TL,Lim VWX,Singhal A,Yeo TW,Leo YS,Vora KA,Casimiro D,Lim B,Tucker-Kellogg L,Rivino L,Newell EW,Fink K

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Tue May 18 00:00:00 EDT 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ontogenic timing, T cell receptor signal strength, and Notch signaling direct γδ T cell functional differentiation in vivo.

Cell reports

Chen ELY,Lee CR,Thompson PK,Wiest DL,Anderson MK,Zúñiga-Pflücker JC

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Tue Jun 08 00:00:00 EDT 2021

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Control of Viral Infection by Natural Killer Cell Inhibitory Receptors.

Cell reports

Parikh BA,Bern MD,Piersma SJ,Yang L,Beckman DL,Poursine-Laurent J,Plougastel-Douglas B,Yokoyama WM

17-0271 was used in Flow cytometry/Cell sorting to show MHC-I-restricted control of murine cytomegalovirus (MCMV) infection in vivo that is NK cell dependent.

Tue Jul 28 00:00:00 EDT 2020

Iterative Single-Cell Analyses Define the Transcriptome of the First Functional Hematopoietic Stem Cells.

Cell reports

Vink CS,Calero-Nieto FJ,Wang X,Maglitto A,Mariani SA,Jawaid W,Göttgens B,Dzierzak E

Tue May 12 00:00:00 EDT 2020

Interleukin-15 Complex Treatment Protects Mice from Cerebral Malaria by Inducing Interleukin-10-Producing Natural Killer Cells.

Immunity

Burrack KS,Huggins MA,Taras E,Dougherty P,Henzler CM,Yang R,Alter S,Jeng EK,Wong HC,Felices M,Cichocki F,Miller JS,Hart GT,Johnson AJ,Jameson SC,Hamilton SE

17-0271-82 was used in Flow Cytometry to identify a regulatory role for cytokine-stimulated NK cells in the prevention of a pathogenic immune response.

Tue Apr 17 00:00:00 EDT 2018

BALDR: a computational pipeline for paired heavy and light chain immunoglobulin reconstruction in single-cell RNA-seq data.

Genome medicine

Upadhyay AA,Kauffman RC,Wolabaugh AN,Cho A,Patel NB,Reiss SM,Havenar-Daughton C,Dawoud RA,Tharp GK,Sanz I,Pulendran B,Crotty S,Lee FE,Wrammert J,Bosinger SE

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Tue Mar 20 00:00:00 EDT 2018

Crosstalks between mTORC1 and mTORC2 variagate cytokine signaling to control NK maturation and effector function.

Nature communications

Wang F,Meng M,Mo B,Yang Y,Ji Y,Huang P,Lai W,Pan X,You T,Luo H,Guan X,Deng Y,Yuan S,Chu J,Namaka M,Hughes T,Ye L,Yu J,Li X,Deng Y

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Mon Nov 19 00:00:00 EST 2018

Identification of Nascent Memory CD8 T Cells and Modeling of Their Ontogeny.

Cell systems

Crauste F,Mafille J,Boucinha L,Djebali S,Gandrillon O,Marvel J,Arpin C

17-0271-82 was used in Flow Cytometry to identify Bcl2 and Mki67 as a marker combination that enables the tracking of nascent memory cells within the effector phase.

Wed Mar 22 00:00:00 EDT 2017

Broadening CD4⁺ and CD8⁺ T Cell Responses against Hepatitis C Virus by Vaccination with NS3 Overlapping Peptide Panels in Cross-Priming Liposomes.

Journal of virology

Filskov J,Mikkelsen M,Hansen PR,Christensen JP,Thomsen AR,Andersen P,Bukh J,Agger EM

17-0271 was used in Flow cytometry/Cell sorting to develop a novel peptide-based nanoparticle vaccine strategy aimed at generating T cell immunity against potential subdominant T cell epitopes that are not efficiently targeted by vaccination with full-length recombinant protein or infection with Hepatitis C virus.

Sat Jul 15 00:00:00 EDT 2017

The breast tumor microenvironment alters the phenotype and function of natural killer cells.

Cellular & molecular immunology

Krneta T,Gillgrass A,Chew M,Ashkar AA

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Thu Sep 01 00:00:00 EDT 2016

NKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20.

The Journal of experimental medicine

Drennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D

17-0271 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells.

Mon Sep 19 00:00:00 EDT 2016

NK cell development requires Tsc1-dependent negative regulation of IL-15-triggered mTORC1 activation.

Nature communications

Yang M,Chen S,Du J,He J,Wang Y,Li Z,Liu G,Peng W,Zeng X,Li D,Xu P,Guo W,Chang Z,Wang S,Tian Z,Dong Z

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Wed Sep 07 00:00:00 EDT 2016

Production of IL-10 by CD4(+) regulatory T cells during the resolution of infection promotes the maturation of memory CD8(+) T cells.

Nature immunology

Laidlaw BJ,Cui W,Amezquita RA,Gray SM,Guan T,Lu Y,Kobayashi Y,Flavell RA,Kleinstein SH,Craft J,Kaech SM

Published figure using CD27 monoclonal antibody (Product # 17-0271-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2015

IL-1β-driven neutrophilia preserves antibacterial defense in the absence of the kinase IKKβ.

Nature immunology

Hsu LC,Enzler T,Seita J,Timmer AM,Lee CY,Lai TY,Yu GY,Lai LC,Temkin V,Sinzig U,Aung T,Nizet V,Weissman IL,Karin M

17-0271 was used in Flow cytometry/Cell sorting to observe neutrophilia in mice with an inducible deletion of IKKβ (Ikkβ(δ) mice) which is believed to be critical for innate immunity.

Tue Feb 01 00:00:00 EST 2011

Plasmid vector-linked maturation of natural killer (NK) cells is coupled to antigen-dependent NK cell activation during DNA-based immunization in mice.

Journal of virology

Zhu R,Mancini-Bourgine M,Zhang XM,Bayard F,Deng Q,Michel ML

17-0271 was used in Flow cytometry/Cell sorting to analyze the mechanisms underlying NK cell activation, and their potential role in adaptive immunity during DNA-based immunization in mice.

Sat Oct 01 00:00:00 EDT 2011

Efficient generation of mucosal and systemic antigen-specific CD8+ T-cell responses following pulmonary DNA immunization.

Journal of virology

Bivas-Benita M,Bar L,Gillard GO,Kaufman DR,Simmons NL,Hovav AH,Letvin NL

17-0271 was used in Flow cytometry/Cell sorting to evaluate the ability of plasmid DNA to induce antigen-specific immune cell responses after pulmonary administration.

Tue Jun 01 00:00:00 EDT 2010

Niche recycling through division-independent egress of hematopoietic stem cells.

The Journal of experimental medicine

Bhattacharya D,Czechowicz A,Ooi AG,Rossi DJ,Bryder D,Weissman IL

17-0271 was used in Flow cytometry/Cell sorting to provide insight as to how HSC replacement can occur despite the residence of endogenous HSCs in niches.

Mon Nov 23 00:00:00 EST 2009

Reevaluating the CD8 T-cell response to herpes simplex virus type 1: involvement of CD8 T cells reactive to subdominant epitopes.

Journal of virology

Sheridan BS,Cherpes TL,Urban J,Kalinski P,Hendricks RL

17-0271 was used in Flow cytometry/Cell sorting to explore the properties of glycoprotein B-nonspecific trigeminal ganglia-resident CD8 T-cells and their role in maintaining viral latency.

Sun Mar 01 00:00:00 EST 2009

DNAM-1 promotes activation of cytotoxic lymphocytes by nonprofessional antigen-presenting cells and tumors.

The Journal of experimental medicine

Gilfillan S,Chan CJ,Cella M,Haynes NM,Rapaport AS,Boles KS,Andrews DM,Smyth MJ,Colonna M

17-0271 was used in Flow cytometry/Cell sorting to study the promoted activation of cytotoxic lymphocytes by nonprofessional antigen-presenting cells and tumors by dnam-1.

Mon Dec 22 00:00:00 EST 2008

Rapid memory CD8+ T-lymphocyte induction through priming with recombinant Mycobacterium smegmatis.

Journal of virology

Hovav AH,Cayabyab MJ,Panas MW,Santra S,Greenland J,Geiben R,Haynes BF,Jacobs WR,Letvin NL

17-0271 was used in Flow cytometry/Cell sorting to investigate novel priming immunogens that might be used in heterologous immunization regimens, showing that priming with Mycobacterium smegmatis leads to rapid memory CD8+ T-lymphocyte induction.

Mon Jan 01 00:00:00 EST 2007

Delayed, asynchronous, and reversible T-lineage specification induced by Notch/Delta signaling.

Genes & development

Taghon TN,David ES,Zúñiga-Pflücker JC,Rothenberg EV

17-0271 was used in Flow cytometry/Cell sorting to study how Notch/Delta signaling is not sufficient for T-lineage specification and commitment, but can be permissive for the maintenance and proliferation of uncommitted progenitors.

Fri Apr 15 00:00:00 EDT 2005

Identification of mast cell progenitors in adult mice.

Proceedings of the National Academy of Sciences of the United States of America

Chen CC,Grimbaldeston MA,Tsai M,Weissman IL,Galli SJ

17-0271 was used in Flow cytometry/Cell sorting to identify mast cell progenitors in mice and investigate their timeline of commitment to the mast cell lineage.

Tue Aug 09 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD antigen 27; CD27; CD27 antigen; CD27L receptor; LPFS2; sCD27; soluble CD27; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFSF7; Tumor necrosis factor receptor superfamily member 7; tumor necrosis factor receptor superfamily, member 7

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Gene Aliases: CD27; S152; S152. LPFS2; T14; TNFRSF7; Tp55

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UniProt ID: (Human) P26842, (Mouse) P41272

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Entrez Gene ID: (Human) 939, (Mouse) 21940, (Rat) 500318

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Function(s)

transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process molecular_function

Process(es)

inflammatory response immune response cell surface receptor signaling pathway brain development release of cytoplasmic sequestered NF-kappaB extrinsic apoptotic signaling pathway via death domain receptors immunoglobulin mediated immune response response to lipopolysaccharide tumor necrosis factor-mediated signaling pathway regulation of cell proliferation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAPK cascade response to ethanol positive regulation of B cell differentiation positive regulation of JNK cascade negative regulation of T cell apoptotic process extrinsic apoptotic signaling pathway apoptotic process B cell proliferation positive regulation of interferon-gamma biosynthetic process positive regulation of T cell differentiation immunoglobulin secretion

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CD27 Monoclonal Antibody (LG.7F9), APC, eBioscience™

CD27 Antibody (17-0271-82) in Flow

Product Details

17-0271-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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