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Invitrogen
CD277 Monoclonal Antibody (eBioBT3.1 (20.1, BT3.1)), eBioscience™
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Application
FIGURE: 1 / 1
CD277 Antibody (14-2779-82) in Flow
Staining of normal human peripheral blood cells with 0.5 µg of Mouse IgG1 kappa Isotype Control Purified (Product # 14-4714-82) (open histogram) or 0.5 µg of Anti-Human CD277 Purified (filled histogram). Cells in the lymphocyte gate were used for analysis.
Product Details
14-2779-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
eBioBT3.1 (20.1, BT3.1)
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_467550
Product Specific Information
Description: The eBioBT3.1 monoclonal antibody recognizes BT3.1, BT3.2 and BT3.3. The BT family of proteins is a subgroup of the Ig superfamily (IgSF). BT is a glycoprotein that forms a major component of the milk fat globule molecule. In addition to BT, there are six other genes that have been discovered which have been sub-divided into the BT2 (BT2.1, BT2.2, and BT2.3) and BT3 (BT3.1, BT3.2, and BT3.3) families. BT3.1, BT3.2, and BT3.3 share 95% mRNA identity. BT3 molecules are constitutively expressed on the cell surface of antigen-presenting cells, and the IgV-like domain of BT3.1 is sufficient for interaction with its counter-receptor. BT3.1 is expressed by T cells, B cells and CD14+ cells.
Applications Reported: This eBioBT3.1 (20.1, BT3.1) antibody has been reported for use in flow cytometric analysis, and immunoprecipitation.
Applications Tested: This eBioBT3.1 (20.1, BT3.1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Purity: Greater than 90%, as determined by SDS-PAGE.
Aggregation: Less than 10%, as determined by HPLC.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
The butyrophilin (BTN) genes are a group of major histocompatibility complex (MHC)-associated genes that encode type I membrane proteins with 2 extracellular immunoglobulin (Ig) domains and an intracellular B30.2 (PRYSPRY) domain. Three subfamilies of human BTN genes are located in the MHC class I region: the single-copy BTN1A1 gene and the BTN2 and BTN3 genes, which have undergone tandem duplication, resulting in 3 copies of each.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: butyrophilin; Butyrophilin subfamily 3 member A1; butyrophilin, subfamily 3, member A1; CD277; dJ45P21.3 (butyrophilin, subfamily 3, member A1)
Gene Aliases: BT3.1; BTF5; BTN3.1; BTN3A1; CD277
UniProt ID: (Human) O00481
Entrez Gene ID: (Human) 11119
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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