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Invitrogen

CD3 Monoclonal Antibody (7D6), TRI-COLOR™

2 Published Figures

13 References

View all (540) CD3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (2)

Application

FIGURE: 1 / 3

CD3 Antibody (MHCD0306) in Flow

Human peripheral blood lymphocytes stained using TRI-COLOR® of anti-human CD3 monoclonal antibody (clone S4.1). The negative control profiles represent unstained cells.

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Product Details

MHCD0306

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

7D6

Immunogen

Human CD3 epsilon

Conjugate

TRI-COLOR™

Excitation/Emission Max

567/671 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 10% sucrose, BSA

Contains

0.1% sodium azide

Storage conditions

4°C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10373680

Product Specific Information

The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5 conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Blocking type I interferon signaling enhances T cell recovery and reduces HIV-1 reservoirs.

The Journal of clinical investigation

Cheng L,Ma J,Li J,Li D,Li G,Li F,Zhang Q,Yu H,Yasui F,Ye C,Tsao LC,Hu Z,Su L,Zhang L

MHCD03065 was used in flow cytometry to study the impact of modulating IFN-I signaling during suppressive combined antiretroviral therapy

Tue Jan 03 00:00:00 UTC 2017

Dual T cell- and B cell-intrinsic deficiency in humans with biallelic RLTPR mutations.

The Journal of experimental medicine

Wang Y,Ma CS,Ling Y,Bousfiha A,Camcioglu Y,Jacquot S,Payne K,Crestani E,Roncagalli R,Belkadi A,Kerner G,Lorenzo L,Deswarte C,Chrabieh M,Patin E,Vincent QB,Müller-Fleckenstein I,Fleckenstein B,Ailal F,Quintana-Murci L,Fraitag S,Alyanakian MA,Leruez-Ville M,Picard C,Puel A,Bustamante J,Boisson-Dupuis S,Malissen M,Malissen B,Abel L,Hovnanian A,Notarangelo LD,Jouanguy E,Tangye SG,Béziat V,Casanova JL

MHCD03065 was used in flow cytometry to identify RLTPR in patients and determine the effects of these mutations on CD4 positive T cells

Mon Oct 17 00:00:00 UTC 2016

Intermediate DNA methylation is a conserved signature of genome regulation.

Nature communications

Elliott G,Hong C,Xing X,Zhou X,Li D,Coarfa C,Bell RJ,Maire CL,Ligon KL,Sigaroudinia M,Gascard P,Tlsty TD,Harris RA,Schalkwyk LC,Bilenky M,Mill J,Farnham PJ,Kellis M,Marra MA,Milosavljevic A,Hirst M,Stormo GD,Wang T,Costello JF

MHCD0306 was used in flow cytometry to show that intermediate DNA methylation is a conserved signature of gene regulation and exon usage

Wed Feb 18 00:00:00 UTC 2015

Anti-inflammatory/regulatory cytokine microenvironment mediated by IL-4 and IL-10 coordinates the immune response in hemophilia A patients infected chronically with hepatitis C virus.

Journal of medical virology

Pimentel JP,Chaves DG,Araújo AR,de Araújo EM,da Silva Fraporti L,Neves WL,Tarragô AM,Torres KL,Gentz SH,Teixeira-Carvalho A,Martins-Filho OA,Malheiro A

Published figure using CD3d monoclonal antibody (Product # MHCD0306) in Flow Cytometry

Sat Jun 01 00:00:00 UTC 2013

Immunological/virological peripheral blood biomarkers and distinct patterns of sleeping quality in chronic hepatitis C patients.

Scandinavian journal of immunology

de Almeida CM,de Lima TA,Castro DB,Torres KL,da Silva Braga W,Peruhype-Magalhães V,Teixeira-Carvalho A,Martins-Filho OA,Malheiro A

MHCD0306 was used in flow cytometry to identify immunological/virological biomarkers associated with sleep quality in patients with chronic hepatitis C.

Sun May 01 00:00:00 UTC 2011

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Cytometry. Part B, Clinical cytometry

Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G

MHCD0306 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.

Tue Jul 01 00:00:00 UTC 2003

Chemokine receptor CCR4 on CD4+ T cells in juvenile rheumatoid arthritis synovial fluid defines a subset of cells with increased IL-4:IFN-gamma mRNA ratios.

Journal of immunology (Baltimore, Md. : 1950)

Thompson SD,Luyrink LK,Graham TB,Tsoras M,Ryan M,Passo MH,Glass DN

MHCD0306 was used in flow cytometry to elucidate the mechanisms that promote recruitment and survival of T cells within the pediatric inflamed joint.

Fri Jun 01 00:00:00 UTC 2001

Multiple co-stimulatory signals are required for triggering proliferation of T cells from human secondary lymphoid tissue.

International immunology

Agrawal SG,Marquet J,Plumas J,Rouard H,Delfau-Larue MH,Gaulard P,Boumsell L,Reyes F,Bensussan A,Farcet JP

MHCD0306 was used in flow cytometry to compare the immune responses of T cells isolated from human secondary lymphoid organs and peripheral blood.

Sun Apr 01 00:00:00 UTC 2001

Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.

Clinical and experimental immunology

Le Cleach L,Delaire S,Boumsell L,Bagot M,Bourgault-Villada I,Bensussan A,Roujeau JC

MHCD0306 was used in flow cytometry to characterize the cells present in the cutaneous blister fluid patients with toxic epidermal necrolysis.

Sat Jan 01 00:00:00 UTC 2000

Perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.

Infection and immunity

Gopinath R,Hanna LE,Kumaraswami V,Perumal V,Kavitha V,Vijayasekaran V,Nutman TB

MHCD0306 was used in flow cytometry to study perturbations in eosinophil homeostasis following treatment of lymphatic filariasis.

Sat Jan 01 00:00:00 UTC 2000

Isolation of CD34+ progenitor cells from peripheral blood by use of an automated immunomagnetic selection system: factors affecting the results.

Transfusion

Martín-Henao GA,Picón M,Amill B,Querol S,González JR,Martínez C,Martino R,Ferrá C,Brunet S,Grañena A,Sierra J,García J

MHCD0306 was used in flow cytometry to discuss methods of CD34+ cell selection from blood.

Sat Jan 01 00:00:00 UTC 2000

Down regulation of CD4 expression following isolation and culture of human monocytes.

Clinical and diagnostic laboratory immunology

Graziani-Bowering GM,Filion LG

MHCD0306 was used in flow cytometry to identify factors that down regulate CD4 on cultured monocytes.

Wed Mar 01 00:00:00 UTC 2000

Retrovirally transduced CD34++ human cord blood cells generate T cells expressing high levels of the retroviral encoded green fluorescent protein marker in vitro.

Blood

Verhasselt B,De Smedt M,Verhelst R,Naessens E,Plum J

MHCD0306 was used in flow cytometry to report an in vitro assay for thymic T-cell development.

Thu Jan 15 00:00:00 UTC 1998

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

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CD3 Monoclonal Antibody (7D6), TRI-COLOR™

CD3 Antibody (MHCD0306) in Flow

Product Details

MHCD0306

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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