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Invitrogen

CD3 Monoclonal Antibody (S4.1), Qdot™ 655

Name: CD3 Monoclonal Antibody (S4.1), Qdot™ 655
Brand: Invitrogen
SKU: Q10012
Price: 742.0 USD
Availability: InStock

1 Published Figure

11 References

View all (540) CD3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

Application

FIGURE: 1 / 2

CD3 Antibody (Q10012) in Flow

Qdot® 655 anti-human CD3 conjugate 655/20 band pass filter

CD3 Antibody in Immunohistochemistry (IHC)

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Product Details

Q10012

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

Assay-dependent

View 6 publications 6 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

S4.1

Immunogen

Human CD3.

Conjugate

Qdot™ 655

Excitation/Emission Max

300/654 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

0.05M borate, pH 8.3, with 1M betaine

Contains

0.05% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2556438

Target Information

The CD3 complex, composed of gamma, delta, epsilon, and zeta subunits, is essential for the assembly, trafficking, and surface expression of the T cell receptor (TCR) complex. These subunits are structurally related members of the immunoglobulin superfamily and are encoded by closely linked genes on human chromosome 11. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells, but not on B or NK cells. The CD3 subunits play a crucial role in transducing antigen-recognition signals into the cytoplasm of T cells. The cytoplasmic tails of CD3 subunits contain a double tyrosine-based motif that associates with cytoplasmic signal transduction molecules, mediating T cell activation through the TCR. Crosslinking of the TCR initiates intracellular biochemical pathways that result in cellular activation, proliferation, and potentially growth arrest and cell survival. CD3 is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes, and Purkinje cells in the cerebellum. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. Defects in the CD3 gene are associated with CD3 immunodeficiency, highlighting its importance in immune function and regulation.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Clonally expanded CD8 T cells characterize amyotrophic lateral sclerosis-4.

Nature

Campisi L,Chizari S,Ho JSY,Gromova A,Arnold FJ,Mosca L,Mei X,Fstkchyan Y,Torre D,Beharry C,Garcia-Forn M,Jiménez-Alcázar M,Korobeynikov VA,Prazich J,Fayad ZA,Seldin MM,De Rubeis S,Bennett CL,Ostrow LW,Lunetta C,Squatrito M,Byun M,Shneider NA,Jiang N,La Spada AR,Marazzi I

Q10484 was used in Flow cytometry/Cell sorting to provide evidence of an antigen-specific CD8 T cell response in ALS4, which could be used to unravel disease mechanisms and as a potential biomarker of disease state.

Wed Jun 01 00:00:00 UTC 2022

Differences in the distribution of CD20, CD3, CD34 and CD45RO in nasal mucosa and polyps from patients with chronic rhinosinusitis.

Molecular medicine reports

Mitroi M,Albulescu D,Capitanescu A,Docea AO,Musat G,Mitroi G,Zlatian O,Tsatsakis A,Tzanakakis G,Spandidos DA,Calina D

Published figure using CD3 monoclonal antibody (Product # Q10012) in Immunohistochemistry

Mon Apr 01 00:00:00 UTC 2019

NK cells produce high levels of IL-10 early after allogeneic stem cell transplantation and suppress development of acute GVHD.

European journal of immunology

Chan YLT,Zuo J,Inman C,Croft W,Begum J,Croudace J,Kinsella F,Maggs L,Nagra S,Nunnick J,Abbotts B,Craddock C,Malladi R,Moss P

Q10484 was used in Flow cytometry/Cell sorting to reveal that robust reconstitution of immunoregulatory natural killer cells by day 14 after allogenic stem cell transplantation is an important determinant of the clinical outcome.

Thu Feb 01 00:00:00 UTC 2018

Predominance of weakly cytotoxic, T-bet^LowEomes^Neg CD8⁺ T-cells in human gastrointestinal mucosa: implications for HIV infection.

Mucosal immunology

Kiniry BE,Ganesh A,Critchfield JW,Hunt PW,Hecht FM,Somsouk M,Deeks SG,Shacklett BL

Q10484 was used in Flow cytometry/Cell sorting to report on the gastrointestinal mucosa site during HIV infection and suggest that rectal CD8+ T-cells are primarily non-cytotoxic, and phenotypically shaped by the tissue microenvironment.

Sat Jul 01 00:00:00 UTC 2017

Replicative fitness of transmitted HIV-1 drives acute immune activation, proviral load in memory CD4+ T cells, and disease progression.

Proceedings of the National Academy of Sciences of the United States of America

Claiborne DT,Prince JL,Scully E,Macharia G,Micci L,Lawson B,Kopycinski J,Deymier MJ,Vanderford TH,Nganou-Makamdop K,Ende Z,Brooks K,Tang J,Yu T,Lakhi S,Kilembe W,Silvestri G,Douek D,Goepfert PA,Price MA,Allen SA,Paiardini M,Altfeld M,Gilmour J,Hunter E

Q10012 was used in flow cytometry to examine the early impact of viral replicative capacity on HIV-1 immunopathogenesis

Tue Mar 24 00:00:00 UTC 2015

Efficacy and safety of CDX-301, recombinant human Flt3L, at expanding dendritic cells and hematopoietic stem cells in healthy human volunteers.

Bone marrow transplantation

Anandasabapathy N,Breton G,Hurley A,Caskey M,Trumpfheller C,Sarma P,Pring J,Pack M,Buckley N,Matei I,Lyden D,Green J,Hawthorne T,Marsh HC,Yellin M,Davis T,Keler T,Schlesinger SJ

Q10012 was used in flow cytometry to report the phase I trial results of CDX-301

Wed Jul 01 00:00:00 UTC 2015

Simultaneous zinc-finger nuclease editing of the HIV coreceptors ccr5 and cxcr4 protects CD4+ T cells from HIV-1 infection.

Blood

Didigu CA,Wilen CB,Wang J,Duong J,Secreto AJ,Danet-Desnoyers GA,Riley JL,Gregory PD,June CH,Holmes MC,Doms RW

Q10012 was used in flow cytometry to use zinc-finger nucleases to simultaneous modify ccr5 and cxcr4 in primary human CD4(+) T cells and introduce these cells into a humanized mouse model of HIV-1 infection.

Thu Jan 02 00:00:00 UTC 2014

Interleukin-7 facilitates HIV-1 transmission to cervico-vaginal tissue ex vivo.

PLoS pathogens

Introini A,Vanpouille C,Lisco A,Grivel JC,Margolis L

Q10484 was used in flow cytometry to study facilitation of HIV-1 transmission to cervico-vaginal tissue ex vivo by interleukin-7

Fri Feb 01 00:00:00 UTC 2013

CD4+CD8+ T cells represent a significant portion of the anti-HIV T cell response to acute HIV infection.

Journal of immunology (Baltimore, Md. : 1950)

Frahm MA,Picking RA,Kuruc JD,McGee KS,Gay CL,Eron JJ,Hicks CB,Tomaras GD,Ferrari G

Q10012 was used in flow cytometry to analyze the proliferation and functional profile of circulating double positive T cells from acutely HIV-infected individuals and chronically HIV-infected viral controllers.

Tue May 01 00:00:00 UTC 2012

IL-7 and IL-21 are superior to IL-2 and IL-15 in promoting human T cell-mediated rejection of systemic lymphoma in immunodeficient mice.

Blood

Markley JC,Sadelain M

Q10012 was used in flow cytometry to compare the therapeutic efficacy of CD19-specific human primary T cells that constitutively express gamma(c)-cytokines

Thu Apr 29 00:00:00 UTC 2010

Quantum dots thermal stability improves simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry.

Journal of immunological methods

Kapoor V,Hakim FT,Rehman N,Gress RE,Telford WG

Q10012 was used in flow cytometry to study the improvement of simultaneous phenotype-specific telomere length measurement by FISH-flow cytometry by quantum dots thermal stability

Fri May 15 00:00:00 UTC 2009

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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