Proteintech

CD34 Polyclonal Antibody

CD34 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD34 Antibody (14486-1-AP) in IHC (P)

Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using (Product # 14486-1-AP) CD34 antibody at dilution of 1:2,000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

CD34 Antibody in Immunohistochemistry (PFA fixed) (IHC (PFA))

Product Image Gallery

Product Details

14486-1-AP

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:1,000

View 14 publications 14 publications

Immunohistochemistry (IHC)

View 32 publications 32 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:500

Immunohistochemistry (PFA fixed) (IHC (PFA))

1:2,000

Immunocytochemistry (ICC/IF)

View 14 publications 14 publications

Flow Cytometry (Flow)

View 2 publications 2 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Not applicable (N/A)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Cat, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

CD34 Fusion Protein Ag5887 (34-287 aa encoded by BC039146)

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.25 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20°C

Shipping conditions

Wet ice (domestic); Dry ice (international)

Product Specific Information

This antibody 14486-1-AP is raised against 33-286 amino acids of full protein.

Immunogen sequence: DNNGTATPE LPTQGTFSNV STNVSYQETT TPSTLGSTSL HPVSQHGNEA TTNITETTVK FTSTSVITSV YGNTNSSVQS QTSVISTVFT TPANVSTPET TLKPSLSPGN VSDLSTTSTS LATSPTKPYT SSSPILSDIK AEIKCSGIRE VKLTQGICLE QNKTSSCAEF KKDRGEGLAR VLCGEEQADA DAGAQVCSLL LAQSEVRPQC LLLVLANRTE ISSKLQLMKK HQSDLKKLGI LDFTEQDVAS HQSYS (34-287 aa encoded by B C039146)

Target Information

CD34 is a highly glycosylated monomeric with a molecular weight range of 111-115 kDa surface protein that is present on many stem cell populations. CD34 is a stem cell marker although its expression on human hematopoietic stem cells is reversible. CD34 may serve as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions. CD34 is possibly an adhesion molecule with a putative role for mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. Further, CD34 could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. CD34 is thought to have a role in presenting carbohydrate ligands to selectins. The intracellular chain of the CD34 antigen is a site of phosphorylation by activated protein kinase C suggesting a putative role in signal transduction. Diseases associated with CD34 dysfunction include dermatofibrosarcoma and neurofibroma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Theranostics

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Oncotarget

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In vivo evaluation of the effects of simultaneous inhibition of GLUT-1 and HIF-1α by antisense oligodeoxynucleotides on the radiosensitivity of laryngeal carcinoma using micro 18F-FDG PET/CT.

Oncotarget

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In vivo evaluation of the effects of simultaneous inhibition of GLUT-1 and HIF-1α by antisense oligodeoxynucleotides on the radiosensitivity of laryngeal carcinoma using micro 18F-FDG PET/CT.

Oncotarget

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Tue May 23 00:00:00 EDT 2017

In vivo evaluation of the effects of simultaneous inhibition of GLUT-1 and HIF-1α by antisense oligodeoxynucleotides on the radiosensitivity of laryngeal carcinoma using micro 18F-FDG PET/CT.

Oncotarget

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A taspine derivative supresses Caco-2 cell growth by competitively targeting EphrinB2 and regulating its pathway.

Oncology reports

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Effect of the vascular endothelial growth factor expression level on angiopoietin-2-mediated nasopharyngeal carcinoma growth.

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Intratumoral neutrophils: a poor prognostic factor for hepatocellular carcinoma following resection.

Journal of hepatology

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Intratumoral neutrophils: a poor prognostic factor for hepatocellular carcinoma following resection.

Journal of hepatology

Li YW,Qiu SJ,Fan J,Zhou J,Gao Q,Xiao YS,Xu YF

Tue Mar 01 00:00:00 EST 2011

Bioinformatics

Protein Aliases: CD34; CD34 antigen; Hematopoietic progenitor cell antigen CD34; Mucosialin; RP11-328D5.2

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Gene Aliases: CD34

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UniProt ID: (Human) P28906

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Entrez Gene ID: (Human) 947

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Function(s)

transcription factor binding carbohydrate binding sulfate binding cell adhesion molecule

Process(es)

tissue homeostasis endothelial cell proliferation glomerular filtration endothelium development cell-matrix adhesion signal transduction regulation of blood pressure cell proliferation positive regulation of gene expression negative regulation of gene expression single organismal cell-cell adhesion hemopoiesis negative regulation of blood coagulation negative regulation of tumor necrosis factor production positive regulation of interleukin-10 production mesangial cell-matrix adhesion paracrine signaling positive regulation of odontogenesis negative regulation of nitric oxide biosynthetic process positive regulation of angiogenesis cell motility regulation of immune response leukocyte migration transdifferentiation vascular wound healing hematopoietic stem cell proliferation positive regulation of transforming growth factor beta production positive regulation of granulocyte colony-stimulating factor production extracellular exosome assembly glomerular endothelium development stem cell proliferation metanephric glomerular mesangial cell differentiation negative regulation of cellular response to heat negative regulation of cellular response to hypoxia negative regulation of interleukin-2 secretion positive regulation of glial cell-derived neurotrophic factor secretion negative regulation of neuron death positive regulation of vasculogenesis

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CD34 Polyclonal Antibody

CD34 Antibody (14486-1-AP) in IHC (P)

Product Details

14486-1-AP

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Not applicable (N/A)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

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