Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Product Details
MA5-16607
Product Specifications
Species Reactivity
Dog
Host/Isotype
Mixed
/ IgG1/IgG2a
Class
Cocktail
Type
Antibody
Clone
CA17.2A12/YKIX302.9
Immunogen
Affinity enriched TCR/CD3 membrane proteins isolated from thymocytes and the T cell line CLGL-9, Canine Concanavilin A activated T-cell blasts
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
purified
Storage buffer
PBS with 5% sucrose, 1% BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2538106
Product Specific Information
CD3e FITC reagent: IgG1 (mouse). CD4 RPE reagent: IgG2a (rat). Clone CA17.2A12 recognizes canine CD3 epsilon, a T cell differentiation antigen expressed on mature T lymphocytes. Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Reconstitute with 1 mL distilled water. After reconstitution, store product undiluted at 4ºC in the dark.
Target Information
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CD3 epsilon; CD3d molecule, delta (CD3-TCR complex); CD3E antigen, epsilon polypeptide (TiT3 complex); CD3g molecule, gamma (CD3-TCR complex); CD4; CD4 antigen (p55); CD4 antigen p55; FLJ18683; Leu-2; MAL; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T8 T-cell antigen
Gene Aliases: CD4
UniProt ID: (Dog) P33705
Entrez Gene ID: (Dog) 611571, (Dog) 479419, (Dog) 442981, (Dog) 489383, (Dog) 403931
positive regulation of cell-matrix adhesion
adaptive immune response
positive regulation of T cell anergy
negative regulation of gene expression
dendrite development
cerebellum development
T cell costimulation
positive regulation of interferon-gamma production
positive regulation of interleukin-4 production
positive regulation of cell-cell adhesion mediated by integrin
negative thymic T cell selection
negative regulation of smoothened signaling pathway
positive regulation of alpha-beta T cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
positive regulation of calcium-mediated signaling
T cell receptor signaling pathway
apoptotic signaling pathway
induction by virus of host cell-cell fusion
cell adhesion
T cell differentiation
macrophage differentiation
maintenance of protein location in cell
positive regulation of I-kappaB kinase/NF-kappaB signaling
T cell selection
positive regulation of monocyte differentiation
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
positive regulation of viral entry into host cell
regulation of T cell activation
regulation of calcium ion transport
cellular response to granulocyte macrophage colony-stimulating factor stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support