Invitrogen

CD4 Monoclonal Antibody (RPA-T4), Super Bright™ 780, eBioscience™

14 Published Figures

16 References

View all (606) CD4 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (14)

FIGURE: 1 / 15

CD4 Antibody (78-0049-42) in Flow

Normal human peripheral blood cells were stained with CD3 Monoclonal Antibody, APC (Product # 17-0038-42) and Mouse IgG1 kappa Isotype Control, Super Bright 780 (Product # 78-4714-82) (left) or CD4 Monoclonal Antibody, Super Bright 780 (right). Cells in the lymphocyte gate were used for analysis.

CD4 Antibody in Immunohistochemistry (IHC)

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Product Details

78-0049-42

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 13 publications 13 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Super Bright™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RPA-T4

Conjugate

Super Bright™ 780 Super Bright™ 780 Super Bright™ 780

Excitation/Emission Max

413/780 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734981

Product Specific Information

Description: The OKT4 monoclonal antibody reacts with human CD4, a 59 kDa cell surface glycoprotein expressed by the majority of thymocytes, a subpopulation of mature T cells (T-helper cells) and in low levels on monocytes. CD4 is a receptor for the human immunodeficiency virus (HIV). The OKT4 antibody recognizes a different epitope than the RPA-T4 monoclonal antibody, and these antibodies do not cross-block binding to each other's respective epitopes.

Applications Tested: This RPA-T4 antibody has been pre-diluted and tested by flow cytometric analysis of normal human peripheral blood cells. This may be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Super Bright 780 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 780 nm. We recommend using a 780/60 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401-42) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

In some experiments, we have observed that compensation values for Super Bright 780-conjugated antibodies are higher in the violet 450/50 channel when using UltraComp eBeads microspheres (Product # 01-2222-42) as compared to single-color stained cells. In such circumstances, we would recommend setting compensation with cells. We have also observed this in some experiments using AbC Total Antibody Compensation beads (Product # A10497).

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Cathepsin S inhibition in dendritic cells prevents Th17 cell differentiation in perivascular adipose tissues following vascular injury in diabetic rats.

Journal of biochemical and molecular toxicology

Peng H,Lv Y,Li C,Cheng Z,He S,Wang C,Liu J

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2023

Rapamycin improves Graves' orbitopathy by suppressing CD4+ cytotoxic T lymphocytes.

JCI insight

Zhang M,Chong KK,Chen ZY,Guo H,Liu YF,Kang YY,Li YJ,Shi TT,Lai KK,He MQ,Ye K,Kahaly GJ,Shi BY,Wang Y

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Wed Feb 08 00:00:00 EST 2023

Induced Human Regulatory T Cells Express the Glucagon-like Peptide-1 Receptor.

Cells

Rode AKO,Buus TB,Mraz V,Al-Jaberi FAH,Lopez DV,Ford SL,Hennen S,Eliasen IP,Klewe IV,Gharehdaghi L,Dragan A,Rosenkilde MM,Woetmann A,Skov L,Ødum N,Bonefeld CM,Kongsbak-Wismann M,Geisler C

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Immunohistochemistry

Fri Aug 19 00:00:00 EDT 2022

Single-cell RNA sequencing reveals the molecular features of peripheral blood immune cells in children, adults and centenarians.

Frontiers in immunology

Zhong J,Ding R,Jiang H,Li L,Wan J,Feng X,Chen M,Peng L,Li X,Lin J,Yang H,Wang M,Li Q,Chen Q

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Mon Jan 30 00:00:00 EST 2023

Single-cell profiling of healthy human kidney reveals features of sex-based transcriptional programs and tissue-specific immunity.

Nature communications

McEvoy CM,Murphy JM,Zhang L,Clotet-Freixas S,Mathews JA,An J,Karimzadeh M,Pouyabahar D,Su S,Zaslaver O,Röst H,Arambewela R,Liu LY,Zhang S,Lawson KA,Finelli A,Wang B,MacParland SA,Bader GD,Konvalinka A,Crome SQ

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Sat Dec 10 00:00:00 EST 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Tue May 10 00:00:00 EDT 2022

Effects of the Antidiabetic Drugs Evogliptin and Sitagliptin on the Immune Function of CD26/DPP4 in Th1 Cells.

Biomolecules & therapeutics

Yoon H,Sung JH,Song MJ

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Murlentamab, a Low Fucosylated Anti-Müllerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation.

Cancers

Prat M,Salon M,Allain T,Dubreuil O,Noël G,Preisser L,Jean B,Cassard L,Lemée F,Tabah-Fish I,Pipy B,Jeannin P,Prost JF,Barret JM,Coste A

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Tue Apr 13 00:00:00 EDT 2021

Characterization and modulation of anti-αβTCR antibodies and their respective binding sites at the βTCR chain to enrich engineered T cells.

Molecular therapy. Methods & clinical development

Kierkels GJJ,van Diest E,Hernández-López P,Scheper W,de Bruin ACM,Frijlink E,Aarts-Riemens T,van Dooremalen SFJ,Beringer DX,Oostvogels R,Kramer L,Straetemans T,Uckert W,Sebestyén Z,Kuball J

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Fri Sep 10 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.

The Journal of international medical research

He J,Xu Y,Yi M,Gu C,Zhu Y,Hu G

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Cytoplasmic Citrate Flux Modulates the Immune Stimulatory NKG2D Ligand MICA in Cancer Cells.

Frontiers in immunology

Møller SH,Mellergaard M,Madsen M,Bermejo AV,Jepsen SD,Hansen MH,Høgh RI,Aldana BI,Desler C,Rasmussen LJ,Sustarsic EG,Gerhart-Hines Z,Daskalaki E,Wheelock CE,Hiron TK,Lin D,O'Callaghan CA,Wandall HH,Andresen L,Skov S

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Neutralization

Fri Apr 30 00:00:00 EDT 2021

Standardized 11-color flow cytometry panel for the functional phenotyping of human T regulatory cells.

Journal of biological methods

Manuszak C,Brainard M,Thrash E,Hodi FS,Severgnini M

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Spontaneous Chitin Accumulation in Airways and Age-Related Fibrotic Lung Disease.

Cell

Van Dyken SJ,Liang HE,Naikawadi RP,Woodruff PG,Wolters PJ,Erle DJ,Locksley RM

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Flow Cytometry

Thu Apr 20 00:00:00 EDT 2017

CXCL13-producing TFH cells link immune suppression and adaptive memory in human breast cancer.

JCI insight

Gu-Trantien C,Migliori E,Buisseret L,de Wind A,Brohée S,Garaud S,Noël G,Dang Chi VL,Lodewyckx JN,Naveaux C,Duvillier H,Goriely S,Larsimont D,Willard-Gallo K

Published figure using CD4 monoclonal antibody (Product # 78-0049-42) in Immunohistochemistry

Fri Jun 02 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; cd4a; fCD4; Leu-3; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4

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Gene Aliases: CD4; CD4mut

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UniProt ID: (Human) P01730

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Entrez Gene ID: (Human) 920

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Function(s)

virus receptor activity glycoprotein binding receptor activity transmembrane signaling receptor activity extracellular matrix structural constituent protein binding zinc ion binding coreceptor activity immunoglobulin binding enzyme binding protein kinase binding MHC class II protein binding protein homodimerization activity protein tyrosine kinase binding

Process(es)

cytokine production adaptive immune response induction by virus of host cell-cell fusion immune response signal transduction cell surface receptor signaling pathway enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol fusion of virus membrane with host plasma membrane T cell differentiation entry into host cell T cell costimulation response to estradiol maintenance of protein location in cell response to vitamin D helper T cell enhancement of adaptive immune response positive regulation of T cell proliferation T cell selection positive regulation of interleukin-2 biosynthetic process positive regulation of protein kinase activity regulation of defense response to virus by virus positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling T cell receptor signaling pathway regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD4 Monoclonal Antibody (RPA-T4), Super Bright™ 780, eBioscience™

CD4 Antibody (78-0049-42) in Flow

Product Details

78-0049-42

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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