Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
MA5-17009 contains a monoclonal mixture of FITC-conjugated CD4 (clone #YTS191.1, Rat IgG2b) and RPE-conjugated CD8 (clone #KT15, Rat IgG2a). This antibody is a dual-color reagent recognizing mouse CD4 and CD8 cell surface antigens. Clone YTS191.1 recognizes murine CD4, a T cell differentiation antigen expressed on thymocytes and helper/inducer T cells in the peripheral blood. Clone KT15 recognizes a non-polymorphic epitope on the murine CD8 alpha chain.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4ºC in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD4; CD4 antigen p55; CD8a; CD8alpha; CD8beta; Leu-2; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4); T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein Lyt-2
Gene Aliases: BB154331; Cd4; Cd8a; L3T4; Ly-2; Ly-35; Ly-4; Ly-B; Lyt-2; Lyt2
UniProt ID: (Mouse) P06332, (Mouse) P01731
Entrez Gene ID: (Mouse) 12504, (Mouse) 12525
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