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Invitrogen

CD43 Monoclonal Antibody (SP55)

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1 Reference

View all (122) CD43 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (4)
Advanced Verification (2)

Application

CD43 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 6

CD43 Antibody (MA5-16339) in ICC/IF

Immunofluorescence analysis of CD43 was performed using 70% confluent log phase U-937 and HT-29 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with CD43 Monoclonal Antibody (SP55) (Product # MA5-16339) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Goat anti-Rabbit IgG (Heavy Chain), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjug... View More

CD43 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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CD43 Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of CD43 was observed in MOLT-4, HEL 92.1.7, U-937 in comparison to HT-29, a negative cell line for CD43, using Anti-CD43 Monoclonal Antibody (Product # MA5-16339) in Western Blot (DOI: 10.1016/j.yexmp.2011.10.009). {RE}

Product Details

MA5-16339

Applications

Tested Dilution

Publications

Western Blot (WB)

1:200-1:250

View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200

Immunocytochemistry (ICC/IF)

1:50-1:100

Flow Cytometry (Flow)

1:100

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Monoclonal

Type

Antibody

Clone

SP55

Immunogen

Synthetic peptide derived from the human CD43.

Conjugate

Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Purification

Protein A/G

Storage buffer

PBS, pH 7.6, with 1% BSA

Contains

0.1% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2537858

Product Specific Information

Heat-mediated antigen retrieval is recommended prior to staining, using a 10mM citrate buffer, pH 6.0, for 10 minutes followed by cooling at room temperature for 20 min. Following antigen retrieval, incubate samples with primary antibody for 30 min at room temperature. A suggested positive control is tonsil tissue.

Target Information

CD43, also known as leukosialin or sialophorin, is a transmembrane mucin-like protein characterized by a high negative charge. It is expressed on the surface of most hematopoietic cells, excluding resting B lymphocytes. CD43 serves both adhesive and anti-adhesive functions, contributing to a repulsive barrier that can interfere with cellular adhesion while also promoting leukocyte aggregation in certain contexts. CD43 is involved in the activation of T cells, B cells, NK cells, and monocytes. Its expression decreases upon activation due to proteolytic processing. The counter-receptor for CD43 is CD169/SIGLEC-1, which is expressed on macrophages. CD43 interacts with actin-binding proteins ezrin and moesin, playing a regulatory role in remodeling T-cell morphology and regulating cell-cell interactions during lymphocyte traffic. It enhances LFA-1 adhesiveness while counteracting LFA-1 induction via other receptors. CD43 signaling induces the functionally active tumor suppressor p53 protein, but in cases of p53 and ARF deficiency, it can promote tumor proliferation and viability. CD43 is an important modulator of leukocyte functions, with dysfunctions associated with diseases such as Wiscott-Aldrich Syndrome and Adenoid Basal Cell Carcinoma.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CD34⁺ HSPCs-derived exosomes contain dynamic cargo and promote their migration through functional binding with the homing receptor E-selectin.

Frontiers in cell and developmental biology

Isaioglou I,Aldehaiman MM,Li Y,Lahcen AA,Rauf S,Al-Amoodi AS,Habiba U,Alghamdi A,Nozue S,Habuchi S,Salama KN,Merzaban JS

Wed Sep 20 00:00:00 UTC 2023

Bioinformatics

Protein Aliases: CD43; Galactoglycoprotein; GALGP; GPL115; Leukocyte sialoglycoprotein; Leukosialin; Sialophorin; sialophorin (gpL115, leukosialin, CD43); sialophorin gpL115

Gene Aliases: CD43; GALGP; GPL115; LSN; SPN

UniProt ID: (Human) P16150

Entrez Gene ID: (Human) 6693

Function(s)

Process(es)

response to protozoan negative regulation of type IV hypersensitivity positive regulation of protein phosphorylation chemotaxis immune response cellular defense response negative regulation of cell adhesion establishment or maintenance of cell polarity signal transduction cell surface receptor signaling pathway T cell costimulation positive regulation of T cell proliferation negative regulation of T cell proliferation positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium negative thymic T cell selection regulation of defense response to virus leukocyte migration apoptotic signaling pathway

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