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            • Primary Antibodies ›
            • CD44 Antibodies

            Invitrogen

            CD44 Monoclonal Antibody (IM7), FITC, eBioscience™

            98 Published Figures
            339 References
            View all (275) CD44 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Tech Support
            Promotion Available
            Learn more
            Catalog # 11-0441-82
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            Price (EUR)
            154,00
             
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            Datasheet
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            Cite CD44 Monoclonal Antibody (IM7), FITC, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (98)
            Application
            CD44 Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 99

            CD44 Antibody (11-0441-82) in Flow

            Staining of C57BL/6 splenocytes with 0.25 µg of Rat IgG2b kappa Isotype Control FITC (Product # 11-4031-82) (open histogram) or 0.25 µg of Anti-Human/Mouse CD44 FITC (filled histogram). Total viable cells were used for analysis.
            View Product

            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            CD44 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)
            CD44 Antibody in Flow Cytometry (Flow)

            Product Details

            11-0441-82

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            -
            View 4 publications 4 publications

            Immunohistochemistry (IHC)

            -
            View 16 publications 16 publications

            Immunocytochemistry (ICC/IF)

            -
            View 17 publications 17 publications

            Flow Cytometry (Flow)

            0.5 µg/test
            View 301 publications 301 publications

            Neutralization (Neu)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Human, Mouse

            Published species

            Fruit fly, Gerbil, Horse, Human, Mouse, Non-human primate, Pig, Rat

            Host/Isotype

            Rat / IgG2b, kappa

            Recommended Isotype Control

            Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            IM7

            Conjugate

            FITC
            • Unconjugated
            • Alexa Fluor 532 
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 737
            • Brilliant Violet 421
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • eFluor 450
            • eFluor 506
            • Functional Grade
            • NovaFluor Blue 510
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Blue 690
            • NovaFluor Blue 725
            • NovaFluor Blue 760
            • NovaFluor Blue 800
            • NovaFluor Red 700
            • NovaFluor Red 710
            • NovaFluor Red 725
            • NovaFluor Red 755
            • NovaFluor UV 765
            • NovaFluor Violet 690
            • NovaFluor Yellow 570
            • NovaFluor Yellow 610
            • NovaFluor Yellow 660
            • NovaFluor Yellow 755
            • NovaFluor Yellow 810
            • PE 
            • PE-Cyanine5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-Cyanine5.5
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Excitation/Emission Max

            498/517 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_465045

            Product Specific Information

            Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.

            Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            CD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Bioinformatics

            Protein Aliases: CD44; CD44 antigen; CD44 molecule; CDw44; cell surface glycoprotein CD44; chondroitin sulfate proteoglycan 8; ECMR-III; ECMRIII; Epican; Extracellular matrix receptor III; GP90 lymphocyte homing/adhesion receptor; hematopoietic cell E- and L-selectin ligand; Heparan sulfate proteoglycan; Hermes antigen; homing function and Indian blood group system; HUTCH-I; hyaluronan receptor; Hyaluronate receptor; Ly-24; Lymphocyte antigen 24; PGP I; PGP-1; PGP-I; PGPI; Phagocytic glycoprotein 1; Phagocytic glycoprotein I; sCD 44; sCD44; soluble CD 44; soluble CD44

            View more View less

            Gene Aliases: AU023126; AW121933; AW146109; CD44; CDW44; CSPG8; ECMR-III; HCELL; HERMES; HUTCH-I; IN; LHR; Ly-24; MC56; MDU2; MDU3; MIC4; Pgp-1; Pgp1

            View more View less

            UniProt ID: (Human) P16070, (Mouse) Q62061

            View more View less

            Entrez Gene ID: (Human) 960, (Mouse) 12505

            View more View less

            Function(s)

            cytokine receptor activity protein binding collagen binding hyaluronic acid binding hyalurononglucosaminidase activity epidermal growth factor receptor binding protein kinase binding cytokine binding phosphoprotein binding transmembrane signal receptor

            Process(es)

            cell-matrix adhesion single organismal cell-cell adhesion extracellular matrix disassembly extracellular matrix organization hyaluronan catabolic process positive regulation of peptidyl-serine phosphorylation positive regulation of heterotypic cell-cell adhesion negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process negative regulation of DNA damage response, signal transduction by p53 class mediator cellular response to fibroblast growth factor stimulus positive regulation of peptidyl-tyrosine phosphorylation leukocyte migration cartilage development interferon-gamma-mediated signaling pathway positive regulation of ERK1 and ERK2 cascade monocyte aggregation positive regulation of monocyte aggregation negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator regulation of cell growth branching involved in ureteric bud morphogenesis wound healing involved in inflammatory response positive regulation of adaptive immune response negative regulation of mature B cell apoptotic process cell adhesion positive regulation of gene expression Wnt signaling pathway cell migration neuron projection development positive regulation of neutrophil apoptotic process macrophage fusion branching involved in prostate gland morphogenesis
            It has to be done as per old AB suggested Products section.
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