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            • Primary Antibodies ›
            • CD45 Antibodies

            Invitrogen

            CD45 Monoclonal Antibody (HI30), PerCP-Cyanine5.5, eBioscience™

            Advanced Verification
            42 Published Figures
            57 References
            View all (443) CD45 antibodies

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            Catalog # 45-0459-42
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            Cite CD45 Monoclonal Antibody (HI30), PerCP-Cyanine5.5, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (42)
            • Advanced Verification (1)
            Application
            CD45 Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 44

            CD45 Antibody (45-0459-42) in Flow

            Staining of normal human peripheral blood cells with staining buffer (autofluorescence) (open histogram) or Anti-Human CD45 PerCP-Cyanine5-5 (filled histogram). Cells in the lymphocyte gate were used for analysis.
            View Product

            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody in Flow Cytometry (Flow)
            CD45 Antibody

            Product Details

            45-0459-42

            Applications
            Tested Dilution
            Publications

            Flow Cytometry (Flow)

            5 µL (0.125 µg)/test
            View 57 publications 57 publications
            Product Specifications

            Species Reactivity

            Human

            Published species

            Human, Mouse, Rabbit

            Host/Isotype

            Mouse / IgG1, kappa

            Recommended Isotype Control

            Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PerCP-Cyanine5.5, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            HI30

            Conjugate

            PerCP-Cyanine5.5
            • Unconjugated
            • Alexa Fluor 532 
            • Alexa Fluor 561
            • Alexa Fluor 647 
            • Alexa Fluor 660 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • eFluor 450
            • eFluor 506
            • FITC
            • NovaFluor Blue 510
            • NovaFluor Blue 585
            • NovaFluor Blue 690
            • NovaFluor Red 710
            • NovaFluor Red 725
            • NovaFluor Red 755
            • NovaFluor Yellow 590
            • NovaFluor Yellow 660
            • NovaFluor Yellow 730
            • NovaFluor Yellow 755
            • PE 
            • PE-Cyanine5
            • PE-Cyanine5.5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-eFluor 710
            • Request custom conjugation

            Excitation/Emission Max

            489/679 nm View spectra spectra

            Form

            Liquid

            Concentration

            5 µL/Test

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2, with BSA

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_10717530

            Product Specific Information

            Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

            Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

            Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
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            Related Products for

            Bioinformatics

            Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

            View more View less

            Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

            View more View less

            UniProt ID: (Human) P08575

            View more View less

            Entrez Gene ID: (Human) 5788

            View more View less

            Function(s)

            protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

            Process(es)

            negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation
            It has to be done as per old AB suggested Products section.
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            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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