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Invitrogen

CD45RO Monoclonal Antibody (UCHL1), APC

Advanced Verification

11 References

View all (53) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Advanced Verification (1)

Application

CD45RO Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 2

CD45RO Antibody (MA1-19782) in Flow

Flow cytometry analysis (surface staining) of human peripheral blood leukocytes by mouse monoclonal antibody anti-CD45R0 UCHL1 (Product # MA1-19782).

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Product Details

MA1-19782

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 7 publications 7 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

10 µL/1x10^6 cells

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Immunogen

Human IL-2 dependent T cells.

View immunogen

Conjugate

APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

Size-exclusion chromatography

Storage buffer

PBS, pH 7.4, with 0.2% BSA

Contains

15mM sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_1955321

Product Specific Information

This antibody recognizes an extracellular epitope of CD45R0, a 180 kDa low molecular weight isoform of the leukocyte common antigen (LCA). The antigen is expressed on a subset of memory/activated T cells and on cortical thymocytes.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Age-associated alteration in Th17 cell response is related to endothelial cell senescence and atherosclerotic cerebral infarction.

American journal of translational research

Li Q,Ding S,Wang YM,Xu X,Shen Z,Fu R,Liu M,Hu C,Zhang C,Cao Q,Wang Y

MA1-19782 was used in Flow cytometry/Cell sorting to demonstrate that IL-17 promotes endothelial cell senescence which is closely related to acute cerebral infarction (ACI) occurrence.

Thu Oct 01 00:00:00 UTC 2020

A giant primary sclerosing lipogranuloma of the scrotum.

Turk patoloji dergisi

Mungan S,Karagüzel E,Turan C,Reıs A

MA1-19782 was used in immunohistochemistry to report on a case of giant primary sclerosing lipogranuloma of the scrotum

Tue Jul 08 00:00:00 UTC 2014

Effector Memory T cells Are Associated With Atherosclerosis in Humans and Animal Models.

Journal of the American Heart Association

Ammirati E,Cianflone D,Vecchio V,Banfi M,Vermi AC,De Metrio M,Grigore L,Pellegatta F,Pirillo A,Garlaschelli K,Manfredi AA,Catapano AL,Maseri A,Palini AG,Norata GD

MA1-19782 was used in flow cytometry to study the relationship between circulating CD4+ T-cell subsets and atherosclerosis

Wed Feb 01 00:00:00 UTC 2012

Axonal projections of mechanoreceptive dorsal root ganglion neurons depend on Ret.

Development (Cambridge, England)

Honma Y,Kawano M,Kohsaka S,Ogawa M

MA1-19782 was used in immunohistochemistry to study the role of Ret in the neurturin-mediated formation of precise axonal projections in the central nervous system

Thu Jul 01 00:00:00 UTC 2010

A patient with clinicopathologic features of small plaque parapsoriasis presenting later with plaque-stage mycosis fungoides: report of a case and comparative retrospective study of 27 cases of "nonprogressive" small plaque parapsoriasis.

Journal of the American Academy of Dermatology

Belousova IE,Vanecek T,Samtsov AV,Michal M,Kazakov DV

MA1-19782 was used in immunohistochemistry to report on a case of small plaque parapsoriasis with plaque-stage mycosis fungoides

Mon Sep 01 00:00:00 UTC 2008

Hyperplasia of hair follicles and other adnexal structures in cutaneous lymphoproliferative disorders: a study of 53 cases, including so-called pseudolymphomatous folliculitis and overt lymphomas.

The American journal of surgical pathology

Kazakov DV,Belousova IE,Kacerovska D,Sima R,Vanecek T,Vazmitel M,Pizinger K,Michal M

MA1-19782 was used in immunohistochemistry to study 53 cases of cutaneous lymphoproliferative disorders

Wed Oct 01 00:00:00 UTC 2008

Up-regulation of expression of the ubiquitin carboxyl-terminal hydrolase L1 gene in human airway epithelium of cigarette smokers.

Cancer research

Carolan BJ,Heguy A,Harvey BG,Leopold PL,Ferris B,Crystal RG

MA1-19782 was used in immunocytochemistry to investigate the expression of carboxyl-terminal hydrolyase L1 gene in human airway epithelium of cigarette smokers

Wed Nov 15 00:00:00 UTC 2006

Primary cutaneous histiocyte and neutrophil-rich CD30+ and CD56+ anaplastic large-cell lymphoma with prominent angioinvasion and nerve involvement in the forehead and scalp of an immunocompetent woman.

Journal of cutaneous pathology

Boudova L,Kazakov DV,Jindra P,Sima R,Vanecek T,Kuntscher V,Vera V,Bouda J,Michal M

MA1-19782 was used in immunohistochemistry to report on a case of utaneous lymphoma of the head co-expressing CD56 and CD30

Tue Aug 01 00:00:00 UTC 2006

Differential expression of human granzymes A, B, and K in natural killer cells and during CD8+ T cell differentiation in peripheral blood.

European journal of immunology

Bratke K,Kuepper M,Bade B,Virchow JC,Luttmann W

MA1-19782 was used in Flow Cytometry to study the expression pattern of human granzymes A, B and K.

Thu Sep 01 00:00:00 UTC 2005

Cutaneous lymphoid hyperplasia and other lymphoid infiltrates of the breast nipple: a retrospective clinicopathologic study of fifty-six patients.

The American Journal of dermatopathology

Boudova L,Kazakov DV,Sima R,Vanecek T,Torlakovic E,Lamovec J,Kutzner H,Szepe P,Plank L,Bouda J,Hes O,Mukensnabl P,Michal M

MA1-19782 was used in immunohistochemistry to study the clinicopathological spectrum of lymphoproliferations and other lymphoid infiltration of the breast nipple

Sat Oct 01 00:00:00 UTC 2005

T-cell-rich B-cell lymphoma: a clinicopathologic study of 21 cases and comparison with 43 cases of diffuse large B-cell lymphoma.

Leukemia research

Aki H,Tuzuner N,Ongoren S,Baslar Z,Soysal T,Ferhanoglu B,Sahinler I,Aydin Y,Ulku B,Aktuglu G

MA1-19782 was used in immunohistochemistry to compare the clinocopathology of T-cell rich B-cell lymphoma and diffuse large B-cell lymphoma

Mon Mar 01 00:00:00 UTC 2004

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

UniProt ID: (Human) P08575

Entrez Gene ID: (Human) 5788

Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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