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CD55 Antibody (MA5-35093) in IHC (P)
Immunohistochemistry analysis of CD55 in paraffin-embedded human placenta. Samples were incubated with CD55 Monoclonal antibody (Product # MA5-35093) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Product Details
MA5-35093
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
ARC0568
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 50-150 of human CD55 (P08174)
Conjugate
Form
Liquid
Concentration
0.21 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2848998
Product Specific Information
Immunogen sequence: EGRTSFPEDT VITYKCEESF VKIPGEKDSV ICLKGSQWSD IEEFCNRSCE VPTRLNSASL KQPYITQNYF PVGTVVEYEC RPGYRREPSL SPKLTCLQNL K
Target Information
CD55 (decay-accelerating factor, DAF) is a GPI-anchored membrane glycoprotein that protects autologous cells from classical and alternative pathway of complement cascade. Bidirectional interactions between CD55 and CD97 are involved in T cell regulation and CD55 can regulate complement when bound to CD97. In tumors, CD55 promotes neoangiogenesis, tumorigenesis, invasiveness and evasion of apoptosis. CD55 is a 70 kDa glycoprotein (in erythrocytes) anchored in the membrane by glycosylphosphatidylinositol tail. In other cells, the apparent molecular weight is somewhat larger for CD55 and has a substantial content of O-glycans. CD55 binds to activated C4b or C3b complement fragments on the cell surface, preventing the assembly and accelerating the decay of both classical and alternative pathways. CD55 carries the Cromer related blood group antigens. Two alternatively spliced transcripts encoding CD55 have been identified, and the predominant transcript encodes a membrane-bound protein expressed on cells exposed to plasma component proteins but an alternatively spliced transcript produces a soluble protein present at much lower levels. CD55 has been studied to have a role in prostate cancer growth and survival.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD55; CD55 antigen; CD55 molecule, decay accelerating factor for complement (Cromer blood group); Complement decay-accelerating factor; RP11-357P18.1
Gene Aliases: CD55; CR; CROM; DAF; TC
UniProt ID: (Human) P08174
Entrez Gene ID: (Human) 1604
ER to Golgi vesicle-mediated transport
complement activation, classical pathway
positive regulation of cytosolic calcium ion concentration
regulation of complement activation
regulation of lipopolysaccharide-mediated signaling pathway
CD4-positive, alpha-beta T cell cytokine production
innate immune response
respiratory burst
negative regulation of complement activation
viral entry into host cell
positive regulation of CD4-positive, alpha-beta T cell activation
positive regulation of CD4-positive, alpha-beta T cell proliferation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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