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Invitrogen
CD61 (Integrin beta 3) Monoclonal Antibody (2C9.G3), Functional Grade, eBioscience™
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-Functional-Grade-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD61 (Integrin beta 3) Antibody (16-0611-82) in Flow")
Product Details
16-0611-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Human,
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
2C9.G3
Conjugate
Functional Grade
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_468984
Product Specific Information
Description: The 2C9.G3 (HMb3-1) monoclonal antibody reacts with mouse and rat CD61, also known as the integrin beta3. CD61 is expressed by activated T cells, granulocytes, and platelet. CD61 associates non-covalently with two integrin alpha subunits; alphaV (CD51) to form Vitronectin Receptor and alphaIib (CD41) to form gpIIb/IIIa. These heterodimeric complexes are responsible for adhesion to extracellular matrix components including fibrinogen, fibronectin, fibronectin, vitronectin, thrombospondin and von Willebrand factor.
Applications Reported: The 2C9.G3 antibody has been reported for use in flow cytometric analysis. 2C9.G3 has also been reported in blocking of ligand binding and some adhesive processes.
Applications Tested: The 2C9.G3 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Storage and handling: Use in a sterile environment.
Filtration: 0.2 µm post-manufacturing filtered.
Purity: Greater than 90%, as determined by SDS-PAGE.
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.
Aggregation: Less than 10%, as determined by HPLC.
Target Information
CD61, also known as GPIIIa or ITGB3, is a 105 kDa glycoprotein expressed on activated T cells, granulocytes, megakaryocytes, platelets, and their precursors. It plays a crucial role in platelet aggregation and functions as a receptor for fibrinogen, fibronectin, von Willebrand factor, vitronectin, and thrombospondin. CD61 forms heterodimeric complexes by associating non-covalently with integrin alpha subunits: alphaV (CD51) to create the Vitronectin Receptor and alphaIIb (CD41) to form gpIIb/IIIa. These complexes are responsible for adhesion to extracellular matrix components, facilitating cell adhesion and cell-surface mediated signaling. CD61 is expressed on platelets and megakaryocytes in association with CD41, and on endothelial cells, monocytes, and osteoclasts in association with CD51. The protein product of CD61 is composed of an alpha chain and a beta chain, which can combine with multiple partners to form different integrins. Its involvement in cell adhesion and signaling underscores its importance in normal physiological processes. Dysfunction of CD61 is associated with diseases such as Glanzmann Thrombasthenia and Platelet type-16 Bleeding Disorder, highlighting its critical role in hemostasis and platelet function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CD61; GPIIIa; integrin beta 3 (Cd61); Integrin beta-3; platelet gpIIIa; Platelet membrane glycoprotein IIIa
Gene Aliases: CD61; GP3A; INGRB3; Itgb3
UniProt ID: (Rat) Q8R2H2, (Mouse) O54890
Entrez Gene ID: (Rat) 29302, (Mouse) 16416
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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