Invitrogen

CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), FITC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

26 Published Figures

32 References

View all (139) Transferrin Receptor (CD71) antibodies

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Datasheet

Protocols

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CD71 (Transferrin Receptor) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 30

CD71 (Transferrin Receptor) Antibody (11-0719-42) in Flow

Staining of Anti-Human CD3 and Anti-Human CD28 Functional Grade Purified (Product # 16-0037-81 and Product # 16-0289-81)-stimulated normal human peripheral blood cells with Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42) (blue histogram) or Anti-Human CD71 (Transferrin Receptor) FITC (purple histogram). Cells in the lymphocyte gate were used for analysis.

CD71 (Transferrin Receptor) Antibody in Immunocytochemistry (ICC/IF)

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Product Details

11-0719-42

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

0.125 µg/mL

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 31 publications 31 publications

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

OKT9 (OKT-9)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1724093

Product Specific Information

Description: The OKT9 monoclonal antibody reacts with human CD71, a 170-180 kDa type II transmembrane protein. CD71, the transferrin receptor, exists as a homodimer on the cell surface and is essential for cellular growth. CD71 is expressed by immature proliferating cells and at low levels on resting mature lymphocytes. Antigen or mitogen stimulation of T and B cells upregulates the expression of CD71.

Applications Reported: This OKT9 (OKT-9) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This OKT9 (OKT-9) antibody has been pre-titrated and tested by flow cytometric analysis of PHA-stimulated human peripheral blood mononuclear cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Rapid increase in transferrin receptor recycling promotes adhesion during T cell activation.

BMC biology

Rossatti P,Redpath GMI,Ziegler L,Samson GPB,Clamagirand CD,Legler DF,Rossy J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Wed Aug 24 00:00:00 EDT 2022

A patient-designed tissue-engineered model of the infiltrative glioblastoma microenvironment.

NPJ precision oncology

Cornelison RC,Yuan JX,Tate KM,Petrosky A,Beeghly GF,Bloomfield M,Schwager SC,Berr AL,Stine CA,Cimini D,Bafakih FF,Mandell JW,Purow BW,Horton BJ,Munson JM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Fri Jul 29 00:00:00 EDT 2022

Decoding the pathogenesis of Diamond-Blackfan anemia using single-cell RNA-seq.

Cell discovery

Wang B,Wang C,Wan Y,Gao J,Ma Y,Zhang Y,Tong J,Zhang Y,Liu J,Chang L,Xu C,Shen B,Chen Y,Jiang E,Kurita R,Nakamura Y,Lim KC,Engel JD,Zhou J,Cheng T,Zhu X,Zhu P,Shi L

11-0719-42 was used in Flow cytometry/Cell sorting to demonstrate that interferon-mediated cell cycle control underlies the clinical efficacy of glucocorticoids.

Tue May 10 00:00:00 EDT 2022

The small GTPase RAB10 regulates endosomal recycling of the LDL receptor and transferrin receptor in hepatocytes.

Journal of lipid research

Khan TG,Ginsburg D,Emmer BT

11-0719-42 was used in Flow Cytometry, Immunocytochemistry to suggest a model in which RAB10 regulates LDL and transferrin uptake by promoting both slow and rapid recycling routes for their respective receptor proteins.

Mon Aug 01 00:00:00 EDT 2022

Common variants in signaling transcription-factor-binding sites drive phenotypic variability in red blood cell traits.

Nature genetics

Choudhuri A,Trompouki E,Abraham BJ,Colli LM,Kock KH,Mallard W,Yang ML,Vinjamur DS,Ghamari A,Sporrij A,Hoi K,Hummel B,Boatman S,Chan V,Tseng S,Nandakumar SK,Yang S,Lichtig A,Superdock M,Grimes SN,Bowman TV,Zhou Y,Takahashi S,Joehanes R,Cantor AB,Bauer DE,Ganesh SK,Rinn J,Albert PS,Bulyk ML,Chanock SJ,Young RA,Zon LI

11-0719 was used in Flow cytometry/Cell sorting to propose that the majority of the RBC-trait-associated variants that reside on transcription-factor-binding sequences fall in STF target sequences, suggesting that the phenotypic variation of RBC traits could stem from altered responsiveness to extracellular stimuli.

Tue Dec 01 00:00:00 EST 2020

Transcriptional States and Chromatin Accessibility Underlying Human Erythropoiesis.

Cell reports

Ludwig LS,Lareau CA,Bao EL,Nandakumar SK,Muus C,Ulirsch JC,Chowdhary K,Buenrostro JD,Mohandas N,An X,Aryee MJ,Regev A,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Tue Jun 11 00:00:00 EDT 2019

Methods for Systematic Identification of Membrane Proteins for Specific Capture of Cancer-Derived Extracellular Vesicles.

Cell reports

Zaborowski MP,Lee K,Na YJ,Sammarco A,Zhang X,Iwanicki M,Cheah PS,Lin HY,Zinter M,Chou CY,Fulci G,Tannous BA,Lai CP,Birrer MJ,Weissleder R,Lee H,Breakefield XO

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Tue Apr 02 00:00:00 EDT 2019

Gene-centric functional dissection of human genetic variation uncovers regulators of hematopoiesis.

eLife

Nandakumar SK,McFarland SK,Mateyka LM,Lareau CA,Ulirsch JC,Ludwig LS,Agarwal G,Engreitz JM,Przychodzen B,McConkey M,Cowley GS,Doench JG,Maciejewski JP,Ebert BL,Root DE,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Thu May 09 00:00:00 EDT 2019

GATA/Heme Multi-omics Reveals a Trace Metal-Dependent Cellular Differentiation Mechanism.

Developmental cell

Tanimura N,Liao R,Wilson GM,Dent MR,Cao M,Burstyn JN,Hematti P,Liu X,Zhang Y,Zheng Y,Keles S,Xu J,Coon JJ,Bresnick EH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Mon Sep 10 00:00:00 EDT 2018

Mechanisms of establishment and functional significance of DNA demethylation during erythroid differentiation.

Blood advances

Bartholdy B,Lajugie J,Yan Z,Zhang S,Mukhopadhyay R,Greally JM,Suzuki M,Bouhassira EE

11-0719 was used in Flow cytometry/Cell sorting to study the mechanisms of establishment and functional significance of DNA demethylation during erythroid differentiation.

Tue Aug 14 00:00:00 EDT 2018

Functional Selectivity in Cytokine Signaling Revealed Through a Pathogenic EPO Mutation.

Cell

Kim AR,Ulirsch JC,Wilmes S,Unal E,Moraga I,Karakukcu M,Yuan D,Kazerounian S,Abdulhay NJ,King DS,Gupta N,Gabriel SB,Lander ES,Patiroglu T,Ozcan A,Ozdemir MA,Garcia KC,Piehler J,Gazda HT,Klein DE,Sankaran VG

11-0719 was used in Flow cytometry/Cell sorting to demonstrate how variation in a single cytokine can lead to biased downstream signaling and can thereby cause human disease.

Thu Mar 09 00:00:00 EST 2017

Variant-aware saturating mutagenesis using multiple Cas9 nucleases identifies regulatory elements at trait-associated loci.

Nature genetics

Canver MC,Lessard S,Pinello L,Wu Y,Ilboudo Y,Stern EN,Needleman AJ,Galactéros F,Brugnara C,Kutlar A,McKenzie C,Reid M,Chen DD,Das PP,A Cole M,Zeng J,Kurita R,Nakamura Y,Yuan GC,Lettre G,Bauer DE,Orkin SH

11-0719 was used in Flow cytometry/Cell sorting to demonstrate the use of multiple designer nucleases and variant-aware library design to interrogate trait-associated regulatory DNA at high resolution.

Sat Apr 01 00:00:00 EDT 2017

Thyroid hormone receptor beta and NCOA4 regulate terminal erythrocyte differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Gao X,Lee HY,Li W,Platt RJ,Barrasa MI,Ma Q,Elmes RR,Rosenfeld MG,Lodish HF

11-0719-42 was used in Flow Cytometry to demonstrate an essential role of thyroid hormone TH during terminal human erythroid cell differentiation; specific depletion of TH from the culture medium completely blocked terminal erythroid differentiation and enucleation.

Tue Sep 19 00:00:00 EDT 2017

Activation of KLF1 Enhances the Differentiation and Maturation of Red Blood Cells from Human Pluripotent Stem Cells.

Stem cells (Dayton, Ohio)

Yang CT,Ma R,Axton RA,Jackson M,Taylor AH,Fidanza A,Marenah L,Frayne J,Mountford JC,Forrester LM

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Genetically engineered red cells expressing single domain camelid antibodies confer long-term protection against botulinum neurotoxin.

Nature communications

Huang NJ,Pishesha N,Mukherjee J,Zhang S,Deshycka R,Sudaryo V,Dong M,Shoemaker CB,Lodish HF

Mon Sep 04 00:00:00 EDT 2017

Hormonal vitamin D up-regulates tissue-specific PD-L1 and PD-L2 surface glycoprotein expression in humans but not mice.

The Journal of biological chemistry

Dimitrov V,Bouttier M,Boukhaled G,Salehi-Tabar R,Avramescu RG,Memari B,Hasaj B,Lukacs GL,Krawczyk CM,White JH

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Fri Dec 15 00:00:00 EST 2017

Knockdown of HSPA9 induces TP53-dependent apoptosis in human hematopoietic progenitor cells.

PloS one

Liu T,Krysiak K,Shirai CL,Kim S,Shao J,Ndonwi M,Walter MJ

11071942 was used in flow cytometry to determine that HSPA9 knockdown is associated with increased TP53 expression and activity

Fri Aug 25 00:00:00 EDT 2017

Targeted Application of Human Genetic Variation Can Improve Red Blood Cell Production from Stem Cells.

Cell stem cell

Giani FC,Fiorini C,Wakabayashi A,Ludwig LS,Salem RM,Jobaliya CD,Regan SN,Ulirsch JC,Liang G,Steinberg-Shemer O,Guo MH,Esko T,Tong W,Brugnara C,Hirschhorn JN,Weiss MJ,Zon LI,Chou ST,French DL,Musunuru K,Sankaran VG

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Thu Jan 07 00:00:00 EST 2016

Enforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.

Stem cells translational medicine

Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM

11-0719 was used in Flow cytometry/Cell sorting to investigate whether activation of exogenous HOXB4 promotes the production of more mature, definitive red blood cells.

Mon Aug 01 00:00:00 EDT 2016

Influenza Vaccination Generates Cytokine-Induced Memory-like NK Cells: Impact of Human Cytomegalovirus Infection.

Journal of immunology (Baltimore, Md. : 1950)

Goodier MR,Rodriguez-Galan A,Lusa C,Nielsen CM,Darboe A,Moldoveanu AL,White MJ,Behrens R,Riley EM

11-0719 was used in Flow cytometry/Cell sorting to examine the effect of seasonal influenza vaccination on natural killer cell function and phenotype in previously unvaccinated individuals.

Fri Jul 01 00:00:00 EDT 2016

Single-cell profiling of human megakaryocyte-erythroid progenitors identifies distinct megakaryocyte and erythroid differentiation pathways.

Genome biology

Psaila B,Barkas N,Iskander D,Roy A,Anderson S,Ashley N,Caputo VS,Lichtenberg J,Loaiza S,Bodine DM,Karadimitris A,Mead AJ,Roberts I

11-0719 was used in Flow cytometry/Cell sorting to clarify the cellular hierarchy in human megakaryocyte/erythroid lineage commitment and highlight the importance of using a combination of single-cell approaches to dissect cellular heterogeneity.

Tue May 03 00:00:00 EDT 2016

PPAR-α and glucocorticoid receptor synergize to promote erythroid progenitor self-renewal.

Nature

Lee HY,Gao X,Barrasa MI,Li H,Elmes RR,Peters LL,Lodish HF

11-0719 was used in Flow cytometry/Cell sorting to study the role of PPAR-α agonists in stimulating self-renewal of early erythroid progenitor cells.

Thu Jun 25 00:00:00 EDT 2015

BCL11A enhancer dissection by Cas9-mediated in situ saturating mutagenesis.

Nature

Canver MC,Smith EC,Sher F,Pinello L,Sanjana NE,Shalem O,Chen DD,Schupp PG,Vinjamur DS,Garcia SP,Luc S,Kurita R,Nakamura Y,Fujiwara Y,Maeda T,Yuan GC,Zhang F,Orkin SH,Bauer DE

11-0719 was used in Flow cytometry/Cell sorting to develop pooled CRISPR-Cas9 guide RNA libraries to perform in situ saturating mutagenesis of human and mouse enhancers.

Thu Nov 12 00:00:00 EST 2015

Acetylated histones contribute to the immunostimulatory potential of neutrophil extracellular traps in systemic lupus erythematosus.

Clinical and experimental immunology

Pieterse E,Hofstra J,Berden J,Herrmann M,Dieker J,van der Vlag J

11-0719 was used in Flow cytometry/Cell sorting to assess the effect of specific histone modifications on NETs ability to drive autoimmune response in systemic lupus erythematous.

Thu Jan 01 00:00:00 EST 2015

Functional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells.

American journal of physiology. Cell physiology

Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2015

DECKO: Single-oligo, dual-CRISPR deletion of genomic elements including long non-coding RNAs.

BMC genomics

Aparicio-Prat E,Arnan C,Sala I,Bosch N,Guigó R,Johnson R

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Fri Oct 23 00:00:00 EDT 2015

Allele-specific analysis of DNA replication origins in mammalian cells.

Nature communications

Bartholdy B,Mukhopadhyay R,Lajugie J,Aladjem MI,Bouhassira EE

11-0719 was used in Flow cytometry/Cell sorting to delineate the mechanisms that control the location and timing of firing of replication origins.

Tue May 19 00:00:00 EDT 2015

Altered translation of GATA1 in Diamond-Blackfan anemia.

Nature medicine

Ludwig LS,Gazda HT,Eng JC,Eichhorn SW,Thiru P,Ghazvinian R,George TI,Gotlib JR,Beggs AH,Sieff CA,Lodish HF,Lander ES,Sankaran VG

11-0719 was used in Flow cytometry/Cell sorting to study the altered translation of GATA1 in cells isolated from patients with Diamond-Blackfan anemia.

Tue Jul 01 00:00:00 EDT 2014

Terminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.

Oncogene

Zhang X,Cruz FD,Terry M,Remotti F,Matushansky I

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Immunofluorescence

Thu May 02 00:00:00 EDT 2013

A feedback loop consisting of microRNA 23a/27a and the β-like globin suppressors KLF3 and SP1 regulates globin gene expression.

Molecular and cellular biology

Ma Y,Wang B,Jiang F,Wang D,Liu H,Yan Y,Dong H,Wang F,Gong B,Zhu Y,Dong L,Yin H,Zhang Z,Zhao H,Wu Z,Zhang J,Zhou J,Yu J

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

α-1,6-Fucosyltransferase (FUT8) inhibits hemoglobin production during differentiation of murine and K562 human erythroleukemia cells.

The Journal of biological chemistry

Sasaki H,Toda T,Furukawa T,Mawatari Y,Takaesu R,Shimizu M,Wada R,Kato D,Utsugi T,Ohtsu M,Murakami Y

Published figure using CD71 (Transferrin Receptor) monoclonal antibody (Product # 11-0719-42) in Flow Cytometry

Fri Jun 07 00:00:00 EDT 2013

Role of ZBP-89 in human globin gene regulation and erythroid differentiation.

Blood

Woo AJ,Kim J,Xu J,Huang H,Cantor AB

11-0719 was used in Flow cytometry/Cell sorting to elucidate the molecular mechanisms underlying erythroid-specific gene regulation.

Thu Sep 29 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD antigen CD71; CD71; p90; T9; TR; Transferrin receptor protein 1

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Gene Aliases: CD71; IMD46; p90; T9; TFR; TFR1; TFRC; TR; TRFR

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UniProt ID: (Human) P02786

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Entrez Gene ID: (Human) 7037

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Function(s)

virus receptor activity glycoprotein binding double-stranded RNA binding transferrin receptor activity protein binding Hsp70 protein binding transferrin transmembrane transporter activity identical protein binding protein homodimerization activity poly(A) RNA binding chaperone binding metalloprotease

Process(es)

regulation of cell growth response to hypoxia cellular iron ion homeostasis acute-phase response aging response to nutrient response to iron ion response to manganese ion osteoclast differentiation positive regulation of B cell proliferation receptor internalization response to retinoic acid transferrin transport cellular response to drug positive regulation of T cell proliferation regulation of cell proliferation positive regulation of bone resorption positive regulation of isotype switching response to copper ion viral entry into host cell iron ion import

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CD71 (Transferrin Receptor) Monoclonal Antibody (OKT9 (OKT-9)), FITC, eBioscience™

CD71 (Transferrin Receptor) Antibody (11-0719-42) in Flow

Product Details

11-0719-42

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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