Search Thermo Fisher Scientific
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
FIGURE: 1 / 1
CD73 Antibody (MA5-24204) in Flow
Flow cytometry of CD73 in Mouse splenocytes were stained with Rat Anti-Mouse CD3 APC‚conjugated Monoclonal Antibody. Samples were incubated in either (A) CD73 monoclonal (Product # MA5-24204) or (B) Rat IgG2A Isotype Control followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab)2 Secondary Antibody.
Product Details
MA5-24204
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2A
Class
Monoclonal
Type
Antibody
Clone
496406
Immunogen
CHO-derived recombinant mouse 5'-Nucleotidase/CD73 Trp29-Lys549
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2607379
Product Specific Information
Reconstitute at 0.5 mg/mL in sterile PBS.
Target Information
CD73 (Ecto-5-prime-nucleotidase, 5-prime-ribonucleotide phosphohydrolase) catalyzes the conversion at neutral pH of purine 5-prime mononucleotides to nucleosides, the preferred substrate being AMP. CD73 consists of a dimer of 2 identical 70 kDa subunits bound externally to the plasma membrane by a glycosyl phosphatidyl inositol linkage. CD73 is used as a marker of lymphocyte differentiation. Consequently, a deficiency of CD73 occurs in a variety of immunodeficiency diseases. Other forms of 5-prime nucleotidase exist in the cytoplasm and lysosomes and can be distinguished from CD73 by their substrate affinities, requirement for divalent magnesium ion, activation by ATP, and inhibition by inorganic phosphate. The CD73 gene has been localized to 6q14-q21 by immunofluorescence and a study of a panel of human x mouse hybrids that contained fragments of chromosome 6 as translocations. Defects in the CD73 gene can lead to the calcification of joints and arteries, and intestinal tuberculosis. Two transcript variants encoding different isoforms of CD73 have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: 5'-deoxynucleotidase; 5'-NT; 5'-nucleotidase; CD73; Ecto-5'-nucleotidase; IMP-specific 5'-nucleotidase; Thymidylate 5'-phosphatase
Gene Aliases: 2210401F01Rik; AI447961; CD73; eNT; NT; Nt5; Nt5e; Nte
UniProt ID: (Mouse) Q61503
Entrez Gene ID: (Mouse) 23959
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support