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CD74 Antibody (MA5-45125) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis using human cervical cancer cells. Fixation involved 2% Formaldehyde for 20 min at RT. Samples were incubated with CD74 monoclonal antibody (Product # MA5-45125) at 1:100 for 12 hours at 4°C, followed by FITC Goat Anti-Mouse (green) at 1:200 for 2 hours at RT. Counterstain used was DAPI (blue) nuclear stain at 1:40,000 for 2 hours at RT. Localization: Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus. Endosome. Lysosome. Magnification: 100x. (A) DAPI (blue) nuclear stain. (B) Ant... View More
Product Details
MA5-45125
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
PIN.1
Immunogen
Human CD74 invariant chain synthetic peptide
Conjugate
Excitation/Emission Max
498/517 nm
View spectra
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
9..09mM sodium bicarbonate/PBS, pH 7.2, with 640.91mM DMSO, 136.36mM ethanolamine
Contains
no preservative
Storage conditions
4° C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2931579
Product Specific Information
1 µg/mL of MA5-45125 was sufficient for detection of CD74 in 20 µg of PALA cell lysates by colorimetric immunolot analysis using goat anti-mouse IgG: AP as the secondary antibody.|Detects approximately 33-35kDa protein doublet corresponding to the molecular mass of the p33 and p35 forms of human CD74.
Target Information
HLA-DR, like other MHC class II molecules, is a transmembrane glycoprotein composed of a 36 kDa alpha chain (DRA) and 27 kDa beta chain (DRB). The alpha chain gene contains 5 exons. Exon 1 encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, and exon 4 encodes the transmembrane domain and the cytoplasmic tail. DRA does not have polymorphisms in the peptide binding part and acts as the sole alpha chain for DRB1, DRB3, DRB4 and DRB5. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. HLA-DR is expressed primarily on antigen presenting cells such as B lymphocytes, monocytes, macrophages, thymic epithelial cells and activated T lymphocytes. Three loci, DR, DQ and DP, encode the major expressed products of the human class II region. The human MHC class II molecules bind intracellularly processed peptides, present them to T-helper cells, and have a critical role in the initiation of the immune response.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD74; CD74 antigen; CD74 antigen (invariant polypeptide of major histocompatibility complex, class II antigen-associated); CD74 molecule, major histocompatibility complex, class II invariant chain; class II-associated invariant chain peptide; dinucleotide microsatellite; gamma chain of class II antigens; H-2 class II histocompatibility antigen gamma chain; histocompatibility: class II antigens, gamma chain of; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; HLA-DR-GAMMA; Ia antigen-associated invariant chain; Ia-associated invariant chain; Ii; invariant polypeptide of major histocompatibility complex, class II antigen-associated; MHC class II-associated invariant chain; MHC HLA-DR gamma chain; p33
Gene Aliases: CD74; CLIP; DHLAG; HLADG; Ia-GAMMA; II
UniProt ID: (Human) P04233, (Mouse) P04441
Entrez Gene ID: (Human) 972, (Mouse) 16149
beta-amyloid binding
cytokine receptor activity
protein binding
cytokine binding
MHC class II protein complex binding
macrophage migration inhibitory factor binding
MHC class II protein binding
MHC class II protein binding, via antigen binding groove
identical protein binding
protein binding involved in protein folding
nitric-oxide synthase binding
scaffold/adaptor protein
activation of MAPK activity
prostaglandin biosynthetic process
positive regulation of cytokine-mediated signaling pathway
positive regulation of dendritic cell antigen processing and presentation
negative regulation of peptide secretion
positive regulation of type 2 immune response
negative regulation of mature B cell apoptotic process
protein complex assembly
intracellular protein transport
defense response
signal transduction
cell proliferation
immunoglobulin mediated immune response
antigen processing and presentation of endogenous antigen
antigen processing and presentation of exogenous peptide antigen via MHC class II
positive regulation of B cell proliferation
macrophage migration inhibitory factor signaling pathway
regulation of macrophage activation
negative regulation of apoptotic process
negative regulation of DNA damage response, signal transduction by p53 class mediator
T cell selection
positive thymic T cell selection
negative thymic T cell selection
negative regulation of T cell differentiation
positive regulation of T cell differentiation
positive regulation of fibroblast proliferation
positive regulation of peptidyl-tyrosine phosphorylation
leukocyte migration
chaperone mediated protein folding requiring cofactor
positive regulation of macrophage cytokine production
positive regulation of ERK1 and ERK2 cascade
positive regulation of neutrophil chemotaxis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator
positive regulation of chemokine (C-X-C motif) ligand 2 production
immune system process
positive regulation of adaptive immune response
protein folding
immune response
cytokine-mediated signaling pathway
antigen processing and presentation
positive regulation of innate immune response
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