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Invitrogen
CD8 alpha Monoclonal Antibody (76-2-11), PE
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Application
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CD8 alpha Antibody (MA5-28715) in Flow
Flow cytometry analysis of CD8 alpha in porcine peripheral blood granulocytes. Cells were stained using CD8 alpha Monoclonal Antibody (76-2-11), PE (Product # MA5-28715) and Mouse Anti-Porcine CD4-FITC.
Product Details
MA5-28715
Product Specifications
Species Reactivity
Pig
Host/Isotype
Mouse
/ IgG2a, kappa
Class
Monoclonal
Type
Antibody
Clone
76-2-11
Immunogen
Fresh dd miniature swine thymocytes
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.1 mg/mL
Purification
purified
Storage buffer
PBS, pH 7.5, with proprietary stabilizer
Contains
<0.1% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2745674
Target Information
CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD8 antigen alpha polypeptide; CD8 antigen beta polypeptide; CD8A; T-cell surface glycoprotein; CD8alpha; CD8beta; fCD8; Leu-2; leu-2a; T-cell surface glycoprotein
Entrez Gene ID: (Pig) 396627, (Pig) 396636
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