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Invitrogen
CD85j (ILT2) Monoclonal Antibody (HP-F1), NovaFluor™ Red 725, eBioscience™
FIGURE: 1 / 2
CD85j (ILT2) Antibody (H043T03R05-A) in Flow
Normal human peripheral blood cells were unstained (left) or stained with CD85j Monoclonal Antibody, NovaFluor Red 725 (right). All cells were co-stained with CD11b Monoclonal Antibody, Super Bright 780 (Product # 78-0118-42). Total viable cells in the monocyte gate were used for analysis, as determined by LIVE/DEAD Blue (Product # L34962). Data was acquired on a 5-laser Cytek Aurora and unmixed with autofluorescence extraction.
Product Details
H043T03R05-A
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
HP-F1
Conjugate
Excitation/Emission Max
636/727 nm
View spectra
Form
Liquid
Concentration
0.1 µg/Test
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
RRID
AB_3098533 (AB_2925930)
Product Specific Information
Description: The monoclonal antibody HP-F1 recognizes CD85j, also known as ILT2, LILRB1, and LIR1. CD85j is a member of the ILT (immunoglobulin-like transcript)/LIR (leukocyte Ig-like receptor)/MIR (monocyte Ig-like receptor) family. CD85j is a single transmembrane glycoprotein with a long cytoplasmic domain containing 4 ITIMs which signal through interactions with SHP-1. Expression is found on myeloid cells (monocytes and dendritic cells) and some lymphoid cells including, subsets of NK, T and B cells. Expression has been correlated with leukemias such as ALL and CLL. Expression on CD8+ cells correlates with effector cell function and plays an important role in viral infections, including HIV, Ebstein Barr and CMV. The ligands for CD85j are MHC Class I molecules such as HLA-G, A, F, B27, E and F.
The monoclonal antibody HP-F1 has been shown to reduce the amount of CD16- dependent cytolytic activity of functional NK cells.
Each product contains 1 vial of NovaFluor conjugate and 1 vial of CellBlox Plus Blocking Buffer .
Applications Reported: This HP-F1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This HP-F1 antibody has been tested by flow cytometric analysis of normal human peripheral cells. This can be used at less than or equal to 5 µL (0.1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
NovaFluor dyes are not compatible with DNA intercalating viability dyes. Do not use viability dyes such as propidium iodide, 7-actinomycin D (7-AAD) and DAPI. Invitrogen LIVE/DEAD Fixable Dead Cell stains are recommended for use with NovaFluor dyes.
This NovaFluor conjugate has been updated to ship with CellBlox Plus Blocking Buffer (Cat. No. (C001T06F01)). This buffer contains formulation improvements over CellBlox. CellBlox Plus Blocking Buffer is required for optimal staining with NovaFluor conjugates and should be used in all experiments where NovaFluor conjugates are used. Whenever possible, we recommend adding CellBlox Plus Blocking Buffer to antibody cocktails/master mixes prior to combining with cells. Add 5 µL per sample (regardless of the number of NovaFluors in your panel) to use the antibody cocktail as intended. For single-color controls, use 5 µL of CellBlox Blocking Buffer per 100 µL of cell sample containing 10^3 to 10^8 cells.
NovaFluor conjugates are based on Phiton™ technology utilizing novel nucleic acid dye structures that allow for engineered fluorescent signatures with consideration for spillover and spread impacts. Learn more
Excitation: 636 nm; Emission: 727 nm; Laser: 633-640 nm (Red) Laser
Target Information
This gene is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is found in a gene cluster at chromosomal region 19q13.4. The encoded protein belongs to the subfamily B class of LIR receptors which contain two or four extracellular immunoglobulin domains, a transmembrane domain, and two to four cytoplasmic immunoreceptor tyrosine-based inhibitory motifs (ITIMs). The receptor is expressed on immune cells where it binds to MHC class I molecules on antigen-presenting cells and transduces a negative signal that inhibits stimulation of an immune response. It is thought to control inflammatory responses and cytotoxicity to help focus the immune response and limit autoreactivity. Multiple transcript variants encoding different isoforms have been found for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD85 antigen-like family member J; CD85j; Ig-like transcript 2; ILT-2; Immunoglobulin-like transcript 2; leucocyte Ig-like receptor B1; Leukocyte immunoglobulin-like receptor subfamily B member 1; leukocyte immunoglobulin-like receptor subfamily B member 1 soluble isoform; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 1; LIR-1; MIR-7; Monocyte/macrophage immunoglobulin-like receptor 7; myeloid inhibitory receptor 7
Gene Aliases: CD85J; ILT-2; ILT2; LILRB1; LIR-1; LIR1; MIR-7; MIR7; PIR-B; PIRB
UniProt ID: (Human) Q8NHL6
Entrez Gene ID: (Human) 10859
negative regulation of T cell mediated cytotoxicity
positive regulation of defense response to virus by host
adaptive immune response
T cell proliferation involved in immune response
negative regulation of cytokine secretion involved in immune response
immune response-inhibiting cell surface receptor signaling pathway
Fc receptor mediated inhibitory signaling pathway
signal transduction
response to virus
positive regulation of gene expression
negative regulation of serotonin secretion
receptor internalization
interferon-gamma production
negative regulation of interferon-gamma production
negative regulation of mononuclear cell proliferation
negative regulation of interferon-beta secretion
negative regulation of T cell proliferation
negative regulation of tumor necrosis factor biosynthetic process
positive regulation of apoptotic process
negative regulation of interferon-gamma biosynthetic process
negative regulation of cell cycle
negative regulation of endocytosis
positive regulation of cytolysis
positive regulation of transcription from RNA polymerase II promoter
negative regulation of natural killer cell mediated cytotoxicity
negative regulation of alpha-beta T cell activation
regulation of immune response
defense response to virus
negative regulation of calcium ion transport
cellular response to lipopolysaccharide
interferon-gamma secretion
dendritic cell differentiation
negative regulation of dendritic cell apoptotic process
negative regulation of interleukin-10 secretion
negative regulation of interleukin-12 secretion
negative regulation of CD8-positive, alpha-beta T cell activation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
positive regulation of gamma-delta T cell activation involved in immune response
negative regulation of dendritic cell differentiation
negative regulation of transforming growth factor-beta secretion
negative regulation of osteoclast development
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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