Hamburger Menu Button
Thermo Fisher Scientific Logo
Sign in
Don't have an account ? Create Account
  • Products
    • Antibodies
    • Cell Culture Media
    • Chemicals
    • Chromatography Columns and Cartridges
    • Lab Equipment
    • Lab Plasticware and Supplies
    • Microplates
    • Oligos, Primers, Probes and Genes
    • TaqMan Real-Time PCR Assays
    • Greener Products
    • See all product categories
  • Applications
    • Bioprocessing
    • Cell Culture and Transfection
    • Cell and Gene Therapy
    • Chromatography
    • Molecular Testing
    • Digital Solutions
    • DNA and RNA Extraction and Analysis
    • Spectroscopy, Elemental and Isotope Analysis
    • See all applications and techniques
  • Services
    • 360° CDMO and CRO Solutions
    • CDMO Services
    • CRO Services
    • Custom Services
    • Enterprise Services
    • Financial and Leasing Services
    • Instrument Services
    • Lab Informatics
    • OEM and Commercial Supply
    • Training Services
    • Unity Lab Services
    • See all services
  • Help and Support
    • How to Order
    • Instrument Support
    • Learning Centers
    • Register for an Account
    • Technical Support Centers
    • See all help and support topics
  • Popular
    • TaqMan Real-Time PCR Assays
      TaqMan Real-Time PCR Assays
    • Antibodies
      Antibodies
    • Oligos, Primers & Probes
      Oligos, Primers & Probes
    • GeneArt Gene Synthesis
      GeneArt Gene Synthesis
    • Cell Culture Plastics
      Cell Culture Plastics
  • Special Offers
  • Contact Us
  • Quick Order
  • Order Status and Tracking
  • Documents and Certificates
Thermo Fisher Scientific Logo

Search Thermo Fisher Scientific

Primary Antibodies
  • Search All
  • Popular Product Areas
  • TaqMan Assays
  • Primary Antibodies
  • Secondary Antibodies
  • Isotype Controls
  • Proteins & Peptides
  • ELISA Kits
  • All Documents & Support
  • Certificates
  • SDS
  • Manuals & Protocols
  • Product FAQs
    Search button Close
            Target of Interest
            Application
            Target Species
            • Order Status
            • Quick Order
            • Sign in
              Sign in
              Don't have an account ? Create Account
              • Account
              • Check Order Status
              • Aspire Member Program
              • Connect: Lab, Data, Apps
              • Custom Products & Projects
              • Services Central

            Disclaimer

            Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

            Loading ...
            • Primary Antibodies ›
            • CD8 Antibodies

            Invitrogen

            CD8a Monoclonal Antibody (53-6.7), Alexa Fluor™ 488, eBioscience™

            98 Published Figures
            215 References
            View all (611) CD8 antibodies
            Datasheet
            Protocols
            Questions & Answers
            Promotion Available
            Learn more
            Catalog # 53-0081-82
            Web quote
            You can generate a web quote (PDF) by signing in to your account and adding products to your cart. View cart and click the 'Generate a web quote' button. With your web quote document, you can get approval for your order and complete your order online.
             
            Price (USD)
            220.15
            Special offer
            Online exclusive
            Ends: 01-Jul-2025
            259.00
            Save 38.85 (15%)
             
            In stock
            Check your price
            Request bulk or custom format
            Join Aspire™ for free antibodies
            Datasheet
            Protocols
            Questions & Answers

            Cite CD8a Monoclonal Antibody (53-6.7), Alexa Fluor™ 488, eBioscience™

            • Antibody Testing Data (1)
            • Published Figures (98)
            Application
            CD8a Antibody in Flow Cytometry (Flow)
            Group 53 Created with Sketch.

            FIGURE: 1 / 99

            CD8a Antibody (53-0081-82) in Flow

            Staining of BALB/c splenocytes with staining buffer (autofluorescence) (open histogram) or 0.25 µg of Anti-Mouse CD8a Alexa Fluor® 488 (filled histogram). Cells in the lymphocyte gate were used for analysis.
            View Product

            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            CD8a Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunocytochemistry (ICC/IF)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Immunohistochemistry (IHC)
            CD8a Antibody in Western Blot (WB)
            CD8a Antibody in Western Blot, Flow Cytometry (WB, Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)
            CD8a Antibody in Flow Cytometry (Flow)

            Product Details

            53-0081-82

            Applications
            Tested Dilution
            Publications

            Western Blot (WB)

            -
            View 2 publications 2 publications

            Immunohistochemistry (IHC)

            -
            View 27 publications 27 publications

            Immunocytochemistry (ICC/IF)

            -
            View 8 publications 8 publications

            Flow Cytometry (Flow)

            0.5 µg/test
            View 174 publications 174 publications

            Immunoprecipitation (IP)

            -
            View 1 publication 1 publication

            Functional Assay (Functional)

            -
            View 2 publications 2 publications

            Miscellaneous PubMed (Misc)

            -
            View 1 publication 1 publication
            Product Specifications

            Species Reactivity

            Mouse

            Published species

            Hamster, Human, Mouse

            Host/Isotype

            Rat / IgG2a, kappa

            Recommended Isotype Control

            Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

            Class

            Monoclonal

            Type

            Antibody

            Clone

            53-6.7

            Conjugate

            Alexa Fluor™ 488
            • Unconjugated
            • Alexa Fluor 532 
            • Alexa Fluor 561
            • Alexa Fluor 660 
            • Alexa Fluor 700 
            • APC 
            • APC-eFluor 780
            • Biotin 
            • Brilliant UV 395
            • Brilliant UV 496
            • Brilliant UV 563
            • Brilliant UV 615
            • Brilliant UV 661
            • Brilliant UV 737
            • Brilliant UV 805
            • Brilliant Violet 421
            • Brilliant Violet 480
            • Brilliant Violet 605
            • Brilliant Violet 650
            • Brilliant Violet 711
            • Brilliant Violet 786
            • eFluor 450
            • eFluor 506
            • eFluor 615
            • eFluor 660
            • FITC
            • Functional Grade
            • NovaFluor Blue 510
            • NovaFluor Blue 585
            • NovaFluor Blue 610-70S
            • NovaFluor Blue 660-120S
            • NovaFluor Blue 725
            • NovaFluor Blue 760
            • NovaFluor Blue 800
            • NovaFluor Red 710
            • NovaFluor UV 765
            • NovaFluor Violet 690
            • NovaFluor Violet 745
            • NovaFluor Violet 800
            • NovaFluor Yellow 590
            • NovaFluor Yellow 610
            • NovaFluor Yellow 660
            • NovaFluor Yellow 690
            • NovaFluor Yellow 700
            • NovaFluor Yellow 730
            • NovaFluor Yellow 810
            • PE 
            • PE-Cyanine5
            • PE-Cyanine5.5
            • PE-Cyanine7
            • PE-eFluor 610
            • PerCP-Cyanine5.5
            • PerCP-eFluor 710
            • Super Bright 436
            • Super Bright 600
            • Super Bright 645
            • Super Bright 702
            • Super Bright 780
            • Request custom conjugation

            Excitation/Emission Max

            499/520 nm View spectra spectra

            Form

            Liquid

            Concentration

            0.5 mg/mL

            Purification

            Affinity chromatography

            Storage buffer

            PBS, pH 7.2

            Contains

            0.09% sodium azide

            Storage conditions

            4°C, store in dark, DO NOT FREEZE!

            Shipping conditions

            Ambient (domestic); Wet ice (international)

            RRID

            AB_469897

            Product Specific Information

            Description: The 53-6.7 monoclonal antibody reacts with the mouse CD8a molecule. CD8a is an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha beta) or as a homodimer (CD8 alpha alpha). A majority of thymocytes and a subpopulation of mature alpha beta TCR T cells express CD8 alpha beta while gamma delta TCR T cells, a subpopulation of intestinal intraepithelial lymphocytes (IELs) and dendritic cells express CD8 alpha alpha. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

            Applications Reported: This 53-6.7 antibody has been reported for use in flow cytometric analysis.

            Applications Tested: This 53-6.7 antibody has been tested by flow cytometric analysis of mouse thymocyte or splenocyte suspensions. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

            Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

            Filtration: 0.2 µm post-manufacturing filtered.

            Target Information

            Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

            For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

            How to build a flow cytometry experiment with Invitrogen Flow Cytometry Panel Builder
            The Invitrogen Flow Cytometry Panel Builder webtool helps you chose antibody clones, fluorophores, fluorescent proteins, and reagents to design your experiment.
            Video Player is loading.
            Current Time 0:00
            /
            Duration 8:21
            Loaded: 1.98%
            0:00
            Stream Type LIVE
            Remaining Time -8:21
             
            1x
            • 2x
            • 1.75x
            • 1.5x
            • 1.25x
            • 1x, selected
            • 0.75x
            • 0.5x
            • Chapters
            • descriptions off, selected
            • captions settings, opens captions settings dialog
            • captions off, selected
            • en (Main), selected

            This is a modal window.

            Beginning of dialog window. Escape will cancel and close the window.

            Text
            Text Background
            Caption Area Background
            Font Size
            Text Edge Style
            Font Family

            End of dialog window.

            This is a modal window. This modal can be closed by pressing the Escape key or activating the close button.

            How to use the Panel Builder

            Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

            Bioinformatics

            Protein Aliases: CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Lymphocyte antigen 3; Lyt-2.1 lymphocyte differentiation antigen (AA at 100); T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-2; T-cell surface glycoprotein Lyt-3

            View more View less

            Gene Aliases: BB154331; Cd8a; Cd8b; Cd8b1; Ly-2; Ly-3; Ly-35; Ly-B; Ly-C; Lyt-2; Lyt-3; Lyt2; Lyt3

            View more View less

            UniProt ID: (Mouse) P01731, (Mouse) P10300

            View more View less

            Entrez Gene ID: (Mouse) 12525, (Mouse) 12526

            View more View less

            Function(s)

            protein binding protein kinase binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

            Process(es)

            immune system process T cell mediated immunity cell surface receptor signaling pathway T cell activation cytotoxic T cell differentiation positive regulation of calcium-mediated signaling defense response to virus
            It has to be done as per old AB suggested Products section.
            guarantee_icon

            Performance Guarantee

            If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

            Learn more
            help_icon

            We're here to help

            Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

            Contact tech support
            Ordering Plus Icon Minus Icon
            • Order Status
            • Order Help
            • Quick Order
            • Supply Center
            • eProcurement
            Support Plus Icon Minus Icon
            • Help and Support
            • Contact Us
            • Technical Support Centers
            • Documents and Certificates
            • Report a Site Issue
            Resources Plus Icon Minus Icon
            • Learning Centers
            • Promotions
            • Events and Webinars
            • Social Media
            About Thermo Fisher Plus Icon Minus Icon
            • About Us
            • Careers
            • Investors
            • News
            • Social Responsibility
            • Trademarks
            • Consumer Health Data Privacy Policy
            Our Portfolio Plus Icon Minus Icon
            • Thermo Scientific
            • Applied Biosystems
            • Invitrogen
            • Gibco
            • Ion Torrent
            • Fisher Scientific
            • Unity Lab Services
            • Patheon
            • PPD
            • Terms & Conditions
            • Privacy Information Center
            • Price & Freight Policy
            • Cookie Preferences

              Your choices regarding cookies on this site

              We and our affiliates and vendors use cookies and similar technologies to operate our sites, recognize visitors to our sites, provide secure log-in, collect statistics to optimize site functionality, and deliver content tailored to your interests. Click Accept All to accept all cookies and go directly to the site, click Reject All to reject all but cookies strictly necessary to the functioning of this site (required cookies), or click on Manage Settings to see detailed descriptions of the types of cookies and choose whether to accept certain cookies while on the site.
            © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
            united states flag icon
            United States

            Your items have has been added!


            Host server : magellan-search-green-7c4bf66445-548cb:80/100.66.76.164:80.
            git-commit: 01fb56a08528fd72f4925b2983aa4df42f9d7994
            git-url: https://github.com/thermofisher/magellan-search
            git-branch: release/2.30.1-2025.05.22-1.0