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Invitrogen

CD8a Monoclonal Antibody (C8/144B), eBioscience™

5 References

View all (614) CD8 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (3)

Application

CD8a Antibody in Immunohistochemistry (Paraffin) (IHC (P))

FIGURE: 1 / 3

CD8a Antibody (14-0085-82) in IHC (P)

Immunohistochemical analysis of CD8a was performed using formalin-fixed paraffin-embedded human tonsil tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for 15 minutes. Foll... View More

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Product Details

14-0085-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 3 publications 3 publications

Immunohistochemistry (Paraffin) (IHC (P))

5 µg/mL

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Mass Spectrometry (MS)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

C8/144B

Conjugate

Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_11150240

Product Specific Information

Description: The monoclonal antibody C8/144B recognizes the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class 1 and through its association with protein tyrosine kinase p56lck plays a role in T cell development and activation of mature T cells.

Applications Reported: This C8/144B antibody has been reported for use in immunohistochemical staining of formalin-fixed paraffin embedded tissue sections.

Applications Tested: This C8/144B antibody has been tested by immunohistochemistry on formalin-fixed paraffin embedded human tonsil tissue using IHC Antigen Retrieval Solution - High pH (Product # 00-4956-58). This can be used at less than or equal to 5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD8, also known as cluster of differentiation 8, is a type I transmembrane glycoprotein of the immunoglobulin family that plays a crucial role in T cell differentiation, activation, and signal transduction. It is expressed as either a heterodimer (CD8 alpha beta) or a homodimer (CD8 alpha alpha). The CD8 alpha beta form is predominantly found on the majority of thymocytes and a subpopulation of mature alpha beta TCR T cells, while the CD8 alpha alpha form is expressed on gamma delta TCR T cells, a subset of intestinal intraepithelial lymphocytes (IELs), and dendritic cells. CD8 functions as a co-receptor for major histocompatibility complex class I (MHC-I) molecules, working alongside the T cell receptor (TCR). The CD8 alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes, and dendritic cells as disulfide-linked homodimers of CD8 alpha. Upon ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), CD8 recruits lymphocyte-specific protein tyrosine kinase (Lck), leading to lymphokine production, increased motility, and activation of cytotoxic T lymphocytes (CTLs). Activated CTLs are vital for clearing pathogens and tumor cells. The differentiation of naive CD8+ T cells into CTLs is strongly enhanced by cytokines such as IL-2, IL-12, and TGF-beta1. Through its interactions with MHC-I and association with protein tyrosine kinase p56lck, CD8 plays a significant role in T cell development and the activation of mature T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Highly multiplexed spatial analysis identifies tissue-resident memory T cells as drivers of ulcerative and immune checkpoint inhibitor colitis.

iScience

van Eijs MJM,Ter Linde JJM,Baars MJD,Amini M,Laclé MM,Brand EC,Delemarre EM,Drylewicz J,Nierkens S,Verheijden RJ,Oldenburg B,Vercoulen Y,Suijkerbuijk KPM,van Wijk F

14-0085-82 was used in Mass Spectrometry to gain spatial proteomic insight in immune checkpoint inhibitor colitis in comparison to ulcerative colitis (UC).

Fri Oct 20 00:00:00 UTC 2023

Adaptive immune responses to SARS-CoV-2 persist in the pharyngeal lymphoid tissue of children.

Nature immunology

Xu Q,Milanez-Almeida P,Martins AJ,Radtke AJ,Hoehn KB,Oguz C,Chen J,Liu C,Tang J,Grubbs G,Stein S,Ramelli S,Kabat J,Behzadpour H,Karkanitsa M,Spathies J,Kalish H,Kardava L,Kirby M,Cheung F,Preite S,Duncker PC,Kitakule MM,Romero N,Preciado D,Gitman L,Koroleva G,Smith G,Shaffer A,McBain IT,McGuire PJ,Pittaluga S,Germain RN,Apps R,Schwartz DM,Sadtler K,Moir S,Chertow DS,Kleinstein SH,Khurana S,Tsang JS,Mudd P,Schwartzberg PL,Manthiram K

14-0085-82 was used in Immunohistochemistry (Paraffin) to provide evidence for persistent tissue-specific immunity to SARS-CoV-2 in the upper respiratory tract of children after infection.

Sun Jan 01 00:00:00 UTC 2023

Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

Cellular and molecular gastroenterology and hepatology

Lutter L,Roosenboom B,Brand EC,Ter Linde JJ,Oldenburg B,van Lochem EG,Horjus Talabur Horje CS,van Wijk F

14-0085-82 was used in Mass cytometry to indicate that therapeutic strategies could have a different impact on the same immune cells depending on the local compartment and presence of an inflammatory milieu, and should be taken into account when investigating short- and long-term effects of new gut T cell-targeting drugs.

Mon Mar 28 00:00:00 UTC 2022

Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes.

eLife

Lin JR,Izar B,Wang S,Yapp C,Mei S,Shah PM,Santagata S,Sorger PK

14-0085 was used in Immunohistochemistry to develop a tissue-based cyclic immunofluorescence method for highly multiplexed immuno-fluorescence imaging of formalin-fixed, paraffin-embedded specimens mounted on glass slides.

Wed Jul 11 00:00:00 UTC 2018

Noninvasive detection of tumor-infiltrating T cells by PET reporter imaging.

The Journal of clinical investigation

McCracken MN,Vatakis DN,Dixit D,McLaughlin J,Zack JA,Witte ON

14-0085 was used in Immunohistochemistry to evaluate the use of hdCK3mut reporter imaging in immunotherapy models.

Fri May 01 00:00:00 UTC 2015

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

UniProt ID: (Human) P01732, (Human) P10966

Entrez Gene ID: (Human) 925, (Human) 926

Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

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