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Invitrogen
CD95 (APO-1/Fas) Monoclonal Antibody (SM1/23), eBioscience™
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Application
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CD95 (APO-1/Fas) Antibody (BMS140) in WB, Flow
International journal of biological sciences 2019 -
Figure 5 Neutrophils arrest cell cycle of tumor cells via Fas/ Fas ligand pathway. A549 cancer cells co-incubated with human neutrophils from healthy donors in the presence or absence of the Fas antagonist (BMS140) and agonist (EOS9.1) at effector to target (E:T) cell ratio of 20:1. Cell cycle and apoptosis of A549 cells were detected by flow cytometry (d, e). Fas L and Fas expression level were further ex... View More
Product Details
BMS140
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
SM1/23
Immunogen
Rec. Human CD95 (APO-1/Fas)
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2-7.4
Contains
no preservative
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10596808
Product Specific Information
Description: BMS140 is suitable as primary antibody for staining in FACS analysis.
BMS140FI can be used for direct staining of cells. BMS140BT can be used in a two-step procedure using the biotin-(strept-)avidin system.
Functional studies:
BMS140 was found to block induction of apoptosis induced by clone SM1/1 or other commercial anti APO-1 antibodies.
Applications Tested: Flow Cytometry, Functional Studies (Blocking).
Purity: >95%.
Target Information
CD95, also known as Fas or APO-1, is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor in the tumor necrosis factor receptor (TNFR) superfamily. It is expressed by a wide range of hematopoietic and non-hematopoietic cells, including monocytes, neutrophils, lymphocytes, and fibroblasts. CD95 also exists in a soluble form weighing approximately 26 kDa. The interaction of CD95 with its ligand, FasL, induces apoptosis, playing a crucial role in peripheral tolerance and anti-viral immune responses. Upon stimulation, CD95 leads to the aggregation of its intracellular Fas-associated death domains (FADD), formation of the death-inducing signaling complex (DISC), and activation of caspases. This cascade results in apoptosis. CD95 is also involved in activating NF-kappaB, MAPK3/ERK1, and MAPK8/JNK, and transduces proliferative signals in normal diploid fibroblasts and T cells. There are at least eight alternatively spliced transcript variants encoding seven distinct isoforms of CD95. Isoforms lacking the transmembrane domain may negatively regulate apoptosis mediated by the full-length isoform. The Fas/Fas ligand system is implicated in several human diseases, including AIDS, hepatitis, and cancer, highlighting its importance in immune regulation and disease pathology.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Apo-1 antigen; APO-1 cell surface antigen; Apo-1 Fas; Apo1 antigen; apoptosis antigen 1; apoptosis signaling receptor FAS; Apoptosis-mediating surface antigen FAS; CD95; CD95 antigen; Fas (TNF receptor superfamily, member 6); Fas AMA; FASLG receptor; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; TNF receptor superfamily member 6; Tumor necrosis factor receptor superfamily member 6; tumor necrosis factor receptor superfamily, member 6
Gene Aliases: ALPS1A; APO-1; APT1; CD95; FAS; FAS1; FASTM; TNFRSF6
UniProt ID: (Human) P25445
Entrez Gene ID: (Human) 355
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