Invitrogen

CD99 Monoclonal Antibody (HO36-1.1)

CD99 Antibody in Immunocytochemistry (ICC/IF)

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CD99 Antibody (MA5-12954) in ICC/IF

Immunofluorescent analysis of CD99 (green) showing staining in the membrane of Jurkat cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD99 monoclonal antibody (Product # MA5-12954) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugate... View More

CD99 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

MA5-12954

Applications

Tested Dilution

Publications

Western Blot (WB)

1:100-1:500

Immunohistochemistry (IHC)

View 4 publications 4 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:200

Immunocytochemistry (ICC/IF)

1:10-1:100

Flow Cytometry (Flow)

2 µg/test

Miscellaneous PubMed (Misc)

View 1 publication 1 publication

Product Specifications

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

HO36-1.1

Immunogen

Purified E-rosette forming cells from human peripheral blood lymphocytes

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.4 mg/mL

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10975896

Product Specific Information

MA5-12954 targets CD99 in WB, IF, FACS and IHC (P) applications and shows reactivity with Human and Rat samples. This antibody is not suitable for Mouse spleen cells in Western blot analysis.

The MA5-12954 immunogen is purified E-rosette forming cells from human peripheral blood lymphocytes.

Target Information

CD99 (E2, MIC2) is a transmembrane glycoprotein that is involved in regulation of T cell addhesive properties and programmed cell death distinct from typical apoptosis course. CD99 roles are specific to certain stages of T cell differentiation such as corticothymocytes. CD99R isoform expression is restricted in the haematopoietic system to T, NK and myeloid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Primary sclerosing epithelioid fibrosarcoma of kidney with variant histomorphologic features: report of 2 cases and review of the literature.

Diagnostic pathology

Ertoy Baydar D,Kosemehmetoglu K,Aydin O,Bridge JA,Buyukeren B,Aki FT

MA5-12954 was used in immunohistochemistry to present two cases of primary sclerosing epithelioid fibrosarcoma of the kidney

Fri Oct 09 00:00:00 EDT 2015

Pericardial synovial sarcoma, a potential for misdiagnosis: clinicopathologic and molecular cytogenetic analysis of three cases with literature review.

American journal of clinical pathology

Cheng Y,Sheng W,Zhou X,Wang J

MA5-12954 was used in immunohistochemistry to report on three cases of pericardial synovial sarcoma

Sun Jan 01 00:00:00 EST 2012

Extraventricular neurocytomas: a morphological and histogenetic consideration. A study of six cases.

Pathology

Agarwal S,Sharma MC,Sarkar C,Suri V,Jain A,Sharma MS,Ailawadhi P,Garg A,Mallick S

MA5-12954 was used in immunohistochemistry to study the morphological and histological features of neurocytomas in extraventricular locations

Wed Jun 01 00:00:00 EDT 2011

Primary sellar neuroblastoma presenting with syndrome of inappropriate secretion of anti-diuretic hormone.

Endocrine pathology

Radotra B,Apostolopoulos V,Sandison A,Hatfield EC,Mendoza N,Moss J,Mehta A,Glaser M,Meeran K,Roncaroli F

MA5-12954 was used in immunohistochemistry to investigate the secretion of anti-diuretic hormone in neuroblastoma

Wed Dec 01 00:00:00 EST 2010

Uterine carcinosarcoma metastatic to the lung as large-cell neuroendocrine carcinoma with synchronous sarcoid granulomatosis.

Lung cancer (Amsterdam, Netherlands)

Froio E,D'Adda T,Fellegara G,Martella E,Caruana P,Pruneri G,Pesci A,Rindi G

MA5-12954 was used in immunohistochemistry to report a clinical case of uterine carcinosarcoma with unique features

Mon Jun 01 00:00:00 EDT 2009

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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