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Application
FIGURE: 1 / 3
CDH9 Antibody (PA5-106548) in ICC/IF
Immunofluorescent analysis of CDH9 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CDH9 polyclonal antibody (Product # PA5-106548) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-106548
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Rat
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CDH9(Accession Q9ULB4), corresponding to amino acid residues L202-T252.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854217
Product Specific Information
Antibody detects endogenous levels of total CDH9.
Target Information
CDH9 (cadherin-9) is a type II classical cadherin from the cadherin superfamily, integral membrane proteins that mediate calcium-dependent cell-cell adhesion. Mature cadherin proteins are composed of a large N-terminal extracellular domain, a single membrane-spanning domain, and a small, highly conserved C-terminal cytoplasmic domain. The extracellular domain consists of 5 subdomains, each containing a cadherin motif, and appears to determine the specificity of the protein's homophilic cell adhesion activity. Type II (atypical) cadherins are defined based on their lack of a HAV cell adhesion recognition sequence specific to type I cadherins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Cadherin; cadherin 9, type 2 (T1-cadherin); Cadherin-9; T1-cadherin
Gene Aliases: CDH9
UniProt ID: (Human) Q9ULB4
Entrez Gene ID: (Human) 1007, (Rat) 29163
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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