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Application
FIGURE: 1 / 3
CDK12 Antibody (PA5-14462) in ICC/IF
Knockdown of Cyclin-dependent kinase 12 was achieved by transfecting HeLa cells with Cyclin-dependent kinase 12 specific siRNA (Silencer® select Product # S28623, S28622). Immunofluorescence analysis was performed on untransfected HeLa cells (panel a,d), transfected with non-specific scrambled siRNA (panels b,e) and transfected with Cyclin-dependent kinase 12 specific siRNA (panel c,f). Cells were fixed, permeabilized, and labelled with CDK12 Polyclonal Antibody (Product # PA5-14462, 1:100 dilution) followed by Donkey anti-Rabbit IgG (H+L)... View More
Product Details
PA5-14462
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human CrkRS (CRK7)
Conjugate
Unconjugated
Form
Liquid
Concentration
2 mg/mL
Purification
Size-exclusion - Dialysis, Ammonium sulfate precipitation
Storage buffer
PBS, pH 7.4
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2245359
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
Target Information
Plays important functions in early cell signaling. Binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AG][AG]-3'), a sequence present in many viral and cellular promoters. Activator of the NF-ELAM1/delta-A site of the E-selectin promoter. Has no intrinsic transcriptional activity, but activates transcription on formation of JUN or FOS heterodimers. Also can bind TRE promoter sequences when heterodimerized with members of the JUN family. Isoform 4/ATF-A0acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter. Isoform 5/ATF-4acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDC2-related kinase 7; CDC2-related kinase, arginine/serine-rich; CDC2-related protein kinase 7; Cell division cycle 2-related protein kinase 7; Cell division protein kinase 12; CrkRS; Cyclin-dependent kinase 12; H KIAA0904; hCDK12; protein kinase for splicing component
Gene Aliases: 1810022J16Rik; AI646528; CDK12; CRK7; CRKR; CRKRS; D11Ertd752e; KIAA0904; Pksc
UniProt ID: (Human) Q9NYV4, (Mouse) Q6ZQ27
Entrez Gene ID: (Human) 51755, (Mouse) 69131
protein kinase activity
cyclin-dependent protein serine/threonine kinase activity
protein binding
ATP binding
RNA polymerase II carboxy-terminal domain kinase activity
cyclin binding
nucleotide binding
protein serine/threonine kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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