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CDK5RAP3 Antibody (PA5-120895) in WB
Western blot analysis of CDK5RAP3 in extracts of various cell lines. Samples were incubated with CDK5RAP3 Polyclonal antibody (Product # PA5-120895) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-120895
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human CDK5RAP3 (NP_788276.1).
Conjugate
Form
Liquid
Concentration
1.43 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2914467
Product Specific Information
Positive test controls include: U-87MG, 293T, LO2, A549, HT-29, Mouse brain, Mouse kidney, Mouse liver, Rat brain. The target is usually found in the following locations: Cytoplasm, Nucleus, centrosome, cytoskeleton, microtubule organizing center.
Immunogen sequence: MEDHQHVPID IQTSKLLDWL VDRRHCSLKW QSLVLTIREK INAAIQDMPE SEEIAQLLSG SYIHYFHCLR ILDLLKGTEA STKNIFGRYS SQRMKDWQEI IALYEKDNTY LVELSSLLVR NVNYEIPSLK KQIAKCQQLQ QEYSRKEEEC QAGAAEMREQ FYHSCKQYGI TGENVRGELL ALVKDLPSQL AEIGAAAQQS LGEAIDVYQA SVGFVCESPT EQVLPMLRFV QKRGNSTVYE WRTGTEPSVV ERPHLEELPE QVAEDAIDWG DFGVEAVSEG TDSGISAEAA GIDWGIFPES
Target Information
This gene encodes a protein that has been reported to function in signaling pathways governing transcriptional regulation and cell cycle progression. It may play a role in tumorigenesis and metastasis. A pseudogene of this gene is located on the long arm of chromosome 20. Alternative splicing results in multiple transcript variants that encode different isoforms. [provided by RefSeq, May 2013].
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CDK5 activator-binding protein C53; CDK5 activator-binding protein C53; CDK5 regulatory subunit associated protein IC53-2; CDK5 regulatory subunit-associated protein 3; IC53-2; ischemic heart CDK5 activator-binding protein C53; LXXLL/leucine-zipper-containing ARF-binding protein; LXXLL/leucine-zipper-containing ARFbinding protein; Protein HSF-27
Gene Aliases: 1810007E24Rik; BC002318; C53; C81486; CDK5RAP3; HSF-27; IC53; LZAP; MST016; MSTP016; OK/SW-cl.114; PP1553
UniProt ID: (Human) Q96JB5, (Mouse) Q99LM2
Entrez Gene ID: (Human) 80279, (Rat) 80278, (Mouse) 80280
regulation of cyclin-dependent protein serine/threonine kinase activity
negative regulation of protein phosphorylation
mitotic G2 DNA damage checkpoint
brain development
cell proliferation
regulation of phosphatase activity
apoptotic nuclear changes
endoplasmic reticulum unfolded protein response
positive regulation of protein ubiquitination
negative regulation of NF-kappaB transcription factor activity
negative regulation of MAP kinase activity
negative regulation of protein kinase activity by regulation of protein phosphorylation
mitotic G2/M transition checkpoint
regulation of neuron differentiation
positive regulation of transcription from RNA polymerase II promoter
protein ufmylation
negative regulation of protein serine/threonine kinase activity
positive regulation of protein localization to nucleus
positive regulation of signal transduction by p53 class mediator
negative regulation of cellular protein catabolic process
positive regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
regulation of mitotic cell cycle
regulation of signal transduction involved in mitotic G2 DNA damage checkpoint
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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