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Application
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CEP164 Antibody (MA5-27823) in WB
Cep 164 antibody [13] detects Cep 164 protein by western blot analysis. Whole cell extracts (30 µg) was separated by 5% SDS-PAGE, and blotted with Cep 164 antibody [13] CEP164 Monoclonal Antibody (13) (Product # MA5-27823) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Product Details
MA5-27823
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
13
Immunogen
Human CEP164.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS
Contains
no preservative
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2735144
Product Specific Information
Positive Control: HeLa
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
CEP164 was initially identified as a centrosomal protein, but other studies have indicated that it also plays a role in the formation of primary cilia, the microtubule-based sensory antennae projecting from the surface of many eukaryotic cells as well as in DNA damage response acting as a mediator protein. CEP164 interacts with both ATR and ATM, proteins that trigger a number of cellular responses including the initiation of DNA damaged-induced cell cycle checkpoints. It is phosphorylated upon replication stress, ultraviolet (UV) radiation, and ionizing radiation silencing of CEP164 significantly reduces the DNA damage-induced phosphorylation of several proteins in the DNA damage-activated signaling cascade and compromises cell survival after UV damage. At least two isoforms of CEP164 are known to exist.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ce164; centrosomal protein 164kDa; Centrosomal protein of 164 kDa; Cep 164; Cep164
Gene Aliases: CEP164; KIAA1052; NPHP15
UniProt ID: (Human) Q9UPV0
Entrez Gene ID: (Human) 22897
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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