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Application
FIGURE: 1 / 4
CES3 Antibody (PA5-47802) in WB
Western blot analysis of CES3 in mouse lung tissue and rat lung tissue. Samples were incubated in CES3 polyclonal antibody (Product # PA5-47802) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. A specific band was detected for Carboxylesterase 3/CES3 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions.
Product Details
PA5-47802
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse,
Rat
Published species
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Carboxylesterase3/CES3 Tyr19-Glu561
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.2 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2606394
Product Specific Information
This antibody detects recombinant mouse Carboxylesterase 3/CES3 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CES1, rhCES2, recombinant mouse (rm) CES2, and rmCES5 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
Target Information
CES3 major lipase in white adipose tissue. Involved in the metabolism of xenobiotics and of natural substrates. Hydrolyzes triacylglycerols and monoacylglycerols, with a preference for monoacylglycerols. The susceptibility of the substrate increases with decreasing acyl chain length of the fatty acid moiety. Catalyzes the synthesis of fatty acid ethyl esters.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Carboxyesterase ES-10; Carboxylesterase 1D; Carboxylesterase 3; carboxylesterase 3, pseudogene 1; Carboxylesterase 3A; EC 3.1.1; EC 3.1.1.1; ES-HVEL; ES-male; Es-male carboxylesterase; ES31FLJ21736; esterase 31; Esterase-31; FAEE synthase; Fatty acid ethyl ester synthase; Liver carboxylesterase 10; Liver carboxylesterase 31; pI 6.1 esterase; TGH; Triacylglycerol hydrolase
Gene Aliases: Ces1; Ces1d; Ces3; Ces3-ps1; Ces31; Ces3a; Es31; TGH
UniProt ID: (Mouse) Q8VCT4, (Rat) P16303, (Mouse) Q63880
Entrez Gene ID: (Mouse) 104158, (Rat) 113902, (Mouse) 382053, (Rat) 689754
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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