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Application
FIGURE: 1 / 7
CH25H Antibody (PA5-70691) in WB
Western blot analysis of CH25H on human fetal brain tissue lysate. The sample was probed with a CH25H polyclonal antibody (Product # PA5-70691) using a primary antibody dilution of 1.0 µg/mL.
Product Details
PA5-70691
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
synthetic peptide directed towards the C-terminal region of human CH25H
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 2% sucrose
Contains
0.09% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2689560
Product Specific Information
This target displays homology in the following species: Cow: 93%; Goat: 83%; Guinea Pig: 93%; Horse: 93%; Human: 100%; Mouse: 93%; Rabbit: 93%; Rat: 93%; Yeast: 75%; Zebrafish: 86%
Target Information
Catalyzes the formation of 25-hydroxycholesterol from cholesterol, leading to repress cholesterol biosynthetic enzymes (PubMed:9852097). Plays a key role in cell positioning and movement in lymphoid tissues: 25-hydroxycholesterol is an intermediate in biosynthesis of 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC), an oxysterol that acts as a ligand for the G protein-coupled receptor GPR183/EBI2, a chemotactic receptor for a number of lymphoid cells. May play an important role in regulating lipid metabolism by synthesizing a corepressor that blocks sterol regulatory element binding protein (SREBP) processing. In testis, production of 25-hydroxycholesterol by macrophages may play a role in Leydig cell differentiation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1.14.99.38; Cholesterol 25-hydroxylase; Cholesterol 25-monooxygenase; h25OH
Gene Aliases: C25H; CH25H
UniProt ID: (Human) O95992
Entrez Gene ID: (Human) 9023
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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