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Invitrogen
CHCHD4 Polyclonal Antibody
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Application
FIGURE: 1 / 1
CHCHD4 Antibody (PA5-89016) in WB
Western blot analysis of CHCHD4 in extracts of various cell lines. Samples were incubated with CHCHD4 Polyclonal antibody (Product # PA5-89016) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Product Details
PA5-89016
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-142 of human CHCHD4 (NP_0010919721)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.71 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2805299
Product Specific Information
Immunogen sequence: MSYCRQEGKD RIIFVTKEDH ETPSSAELVA DDPNDPYEEH GLILPNGNIN WNCPCLGGMA SGPCGEQFKS AFSCFHYSTE EIKGSDCVDQ FRAMQECMQK YPDLYPQEDE DEEEEREKKP AEQAEETAPI EATATKEEEG SS; Positive Samples: LO2, 293T, Mouse liver, Mouse heart, Mouse pancreas; Cellular Location: Mitochondrion intermembrane space
Target Information
CHCHD4, a component of human mitochondria, belongs to a protein family whose members share 6 highly conserved cysteine residues constituting a -CXC-CX(9)C-CX(9)C- motif in the C terminus (Hofmann et al., 2005).
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Coiled-coil-helix-coiled-coil-helix domain-containing protein 4; mitochondrial intermembrane space import and assembly 40 homolog; Mitochondrial intermembrane space import and assembly protein 40; translocase of inner mitochondrial membrane 40 homolog
Gene Aliases: 2410012P20Rik; 2810014D17Rik; AI838740; CHCHD4; MIA40; TIMM40
UniProt ID: (Human) Q8N4Q1, (Mouse) Q8VEA4, (Rat) Q5BJN5
Entrez Gene ID: (Human) 131474, (Mouse) 72170, (Rat) 312559
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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