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Application
FIGURE: 1 / 1
CHKA Antibody (PA5-109529) in WB
Western blot analysis of CHKA in extracts of various cell lines. Samples were incubated with CHKA Polyclonal antibody (Product # PA5-109529) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-109529
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-170 of human CHKA (NP_001268.2).
Conjugate
Unconjugated
Form
Liquid
Concentration
1.41 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.01% thimerosal
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2854940
Product Specific Information
Immunogen sequence: MKTKFCTGGE AEPSPLGLLL SCGSGSAAPA PGVGQQRDAA SDLESKQLGG QQPPLALPPP PPLPLPLPLP QPPPPQPPAD EQPEPRTRRR AYLWCKEFLP GAWRGLREDE FHISVIRGGL SNMLFQCSLP DTTATLGDEP RKVLLRLYGA ILQMRSCNKE GSEQAQKENE
Target Information
The major pathway for the biosynthesis of phosphatidylcholine occurs via the CDP-choline pathway. Choline kinase alpha is the initial enzyme in the sequence and may play a regulatory role. This protein also catalyzes the phosphorylation of ethanolamine.
⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: CHETK-alpha; Choline kinase alpha; choline kinase R; choline/ethanolamine kinase alpha; CK; EK; Ethanolamine kinase
Gene Aliases: Chetk-alpha; CHK; CHKA; ChoK; CK; CK-R; CK/EK-alpha; CKI; Ckr; EK; EtnK-alpha
UniProt ID: (Human) P35790, (Mouse) Q8BPL3, (Rat) Q01134
Entrez Gene ID: (Human) 1119, (Mouse) 12660, (Rat) 29194
choline kinase activity
cholinesterase activity
ethanolamine kinase activity
signal transducer activity
ATP binding
drug binding
choline binding
protein homodimerization activity
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
kinase
ethanolamine metabolic process
lipid metabolic process
phosphatidylethanolamine biosynthetic process
phosphatidylcholine biosynthetic process
CDP-choline pathway
lipid transport
signal transduction
response to toxic substance
phosphorylation
choline metabolic process
response to 3-methylcholanthrene
phospholipid biosynthetic process
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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