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Application
FIGURE: 1 / 3
CLCA1 Antibody (PA5-115025) in ICC/IF
Immunocytochemistry analysis of CLCA1 in HeLa cells. Samples were treated with PFA, permeabilized in 0.1% Triton X-100, blocked in 10% serum (45 min at 25°C), and incubated with polyclonal antibody (Product # PA5-115025) at a dilution of 1:200 (1 hr at 37°C). Secondary staining was applied with mouse anti-beta tubulin; AlexaFluor 594 conjugated goat anti-rabbit IgG (Red); AlexaFluor 88 conjugated goat anti-mouse IgG (Green) and DAPI (blue) using a dilution of 1:200 (1 hr at 37°C).
Product Details
PA5-115025
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CLCA1(Accession A8K7I4), corresponding to amino acid residues V850-K900.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2899661
Product Specific Information
Antibody detects endogenous levels of total CLCA1.
Target Information
This gene encodes a member of the calcium sensitive chloride conductance protein family. To date, all members of this gene family map to the same region on chromosome 1p31-p22 and share a high degree of homology in size, sequence, and predicted structure, but differ significantly in their tissue distributions. The encoded protein is expressed as a precursor protein that is processed into two cell-surface-associated subunits, although the site at which the precursor is cleaved has not been precisely determined. The encoded protein may be involved in mediating calcium-activated chloride conductance in the intestine.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CaCC-1; Calcium-activated chloride channel family member 1; Calcium-activated chloride channel family member 3; Calcium-activated chloride channel protein 1; Calcium-activated chloride channel regulator 1; calcium-dependent chloride channel-1; chloride channel calcium activated 2; chloride channel calcium activated 3; chloride channel regulator 1; chloride channel, calcium activated, family member 1; CLCA family member 1, chloride channel regulator; hCLCA1; mCLCA3; Protein gob-5
Gene Aliases: CACC; CaCC-1; CACC1; CLCA1; Clca2; Clca3; CLCRG1; gob-5; GOB5; hCaCC-1; hCLCA1
UniProt ID: (Human) A8K7I4, (Mouse) Q9D7Z6
Entrez Gene ID: (Human) 1179, (Mouse) 23844
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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