Invitrogen

CLPP Polyclonal Antibody

CLPP Antibody in Immunocytochemistry (ICC/IF)

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CLPP Antibody (PA5-52722) in ICC/IF

Immunofluorecent analysis of CLPP in human cell line U-251 MG using CLPP Polyclonal Antibody (Product # PA5-52722). Staining shows localization to mitochondria.

CLPP Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CLPP Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HEK293 cells were transfected with, control siRNA, CLPP siRNA probe #1, and a decrease in signal intensity was observed in Western Blot application using Anti-CLPP Polyclonal Antibody (Product # PA5-52722) used at a dilution of 0.4 µg/mL. Anti-PPIB was used as a loading control. {KD}

Product Details

PA5-52722

Applications

Tested Dilution

Publications

Western Blot (WB)

0.04-0.4 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:500

Immunocytochemistry (ICC/IF)

0.25-2 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein corresponding to Human CLPP. Recombinant protein control fragment (Product #RP-89221).

View immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2639906

Product Specific Information

Immunogen sequence: MGSLLLAAGT PGMRHSLPNS RIMIHQPSGG ARGQATDIAI QAEEIMKLKK QLYNIYAKHT KQSLQVIESA MERDRYMSPM EAQEFGILDK VLVHPPQDGE DEPTLVQKEP VEAAPAAEPV PA

Highest antigen sequence identity to the following orthologs: Mouse - 91%, Rat - 92%.

Target Information

The protein encoded by this gene belongs to the peptidase family S14 and hydrolyzes proteins into small peptides in the presence of ATP and magnesium. The protein is transported into mitochondrial matrix and is associated with the inner mitochondrial membrane.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

(+)-Lipoic acid reduces mitochondrial unfolded protein response and attenuates oxidative stress and aging in an in vitro model of non-alcoholic fatty liver disease.

Journal of translational medicine

Longhitano L,Distefano A,Musso N,Bonacci P,Orlando L,Giallongo S,Tibullo D,Denaro S,Lazzarino G,Ferrigno J,Nicolosi A,Alanazi AM,Salomone F,Tropea E,Barbagallo IA,Bramanti V,Li Volti G,Lazzarino G,Torella D,Amorini AM

Published figure using CLPP polyclonal antibody (Product # PA5-52722) in Western Blot

Sat Jan 20 00:00:00 UTC 2024

CHCHD2 harboring Parkinson's disease-linked T61I mutation precipitates inside mitochondria and induces precipitation of wild-type CHCHD2.

Human molecular genetics

Cornelissen T,Spinazzi M,Martin S,Imberechts D,Vangheluwe P,Bird M,De Strooper B,Vandenberghe W

PA5-52722 was used in Western Blot to show that the T61I mutation renders mutant CHCHD2 insoluble inside mitochondria, suggesting loss of function of the mutant protein.

Fri May 08 00:00:00 UTC 2020

Bioinformatics

Protein Aliases: ATP-dependent Clp protease proteolytic subunit, mitochondrial; ATP-dependent protease ClpAP, proteolytic subunit, human; caseinolytic mitochondrial matrix peptidase proteolytic subunit; caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; caseinolytic protease, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; Endopeptidase Clp; putative ATP-dependent Clp protease proteolytic subunit, mitochondrial

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Gene Aliases: AU019820; CLPP; D17Wsu160e; DFNB81; PRLTS3

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UniProt ID: (Human) Q16740, (Mouse) O88696

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Entrez Gene ID: (Human) 8192, (Rat) 301117, (Mouse) 53895

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Function(s)

serine-type endopeptidase activity protein binding peptidase activity identical protein binding serine-type peptidase activity hydrolase activity serine protease

Process(es)

protein homooligomerization proteolysis involved in cellular protein catabolic process proteolysis misfolded or incompletely synthesized protein catabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CLPP Polyclonal Antibody

CLPP Antibody (PA5-52722) in ICC/IF

Product Details

PA5-52722

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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