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Invitrogen

CLPX Polyclonal Antibody

1 Published Figure

2 References

View all (10) CLPX antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (1)

Application

FIGURE: 1 / 1

CLPX Antibody (PA5-79052) in WB

Cells 2021 - Figure 4 ( A ) Analysis of both PRLTS3 patients' (in triplicates) versus 7 healthy controls' skin fibroblast proteome profiles in volcano plots, highlighting the accumulated proteins in mitochondria. The significance threshold at 1.3 on the Y -axis corresponds to the p -value 0.05, and the X -axis shows the traditional cut-off at 1.5-fold effects, although genetic evidence has demonstrated a neurodegenerati... View More

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Product Details

PA5-79052

Applications

Tested Dilution

Publications

Western Blot (WB)

0.1-0.5 µg/mL

View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

0.5-1 µg/mL

Immunohistochemistry (Frozen) (IHC (F))

0.5-1 µg/mL

Immunocytochemistry (ICC/IF)

2 µg/mL

Flow Cytometry (Flow)

1-3 µg/1x10^6 cells

ELISA (ELISA)

0.1-0.5 µg/mL

Product Specifications

Species Reactivity

Human, Mouse, Rat

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

E. coli-derived human CLPX recombinant protein (Position: A337-E574).

View immunogen

Conjugate

Unconjugated

Form

Lyophilized

Concentration

500 µg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 4mg trehalose

Contains

0.05mg sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2746168

Product Specific Information

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human HepG2 whole cell. IHC: human placenta tissue, human rectal cancer tissue, mouse cardiac muscle tissue. ICC/IF: U20S cell. Flow: Hela cell.

Target Information

ATP-dependent specificity component of the Clp protease. It directs the protease to specific substrates. Can perform chaperone functions in the absence of clpP.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

CLPP Depletion Causes Diplotene Arrest; Underlying Testis Mitochondrial Dysfunction Occurs with Accumulation of Perrault Proteins ERAL1, PEO1, and HARS2.

Cells

Key J,Gispert S,Koornneef L,Sleddens-Linkels E,Kohli A,Torres-Odio S,Koepf G,Amr S,Reichlmeir M,Harter PN,West AP,Münch C,Baarends WM,Auburger G

PA5-79052 was used in Western Blotting to propose that the loss of CLPP leads to the extrusion of mitochondrial nucleotide-binding proteins to cytosol and nucleus, affecting late meiotic prophase progression, and causing cell death prior to M-phase entry.

Thu Dec 22 00:00:00 UTC 2022

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Cells

Key J,Torres-Odio S,Bach NC,Gispert S,Koepf G,Reichlmeir M,West AP,Prokisch H,Freisinger P,Newman WG,Shalev S,Sieber SA,Wittig I,Auburger G

Published figure using CLPX polyclonal antibody (Product # PA5-79052) in Western Blot

Mon Nov 29 00:00:00 UTC 2021

Inactivity of Peptidase ClpP Causes Primary Accumulation of Mitochondrial Disaggregase ClpX with Its Interacting Nucleoid Proteins, and of mtDNA.

Cells

Key J,Torres-Odio S,Bach NC,Gispert S,Koepf G,Reichlmeir M,West AP,Prokisch H,Freisinger P,Newman WG,Shalev S,Sieber SA,Wittig I,Auburger G

Published figure using CLPX polyclonal antibody (Product # PA5-79052) in Western Blot

Mon Nov 29 00:00:00 UTC 2021

Bioinformatics

Protein Aliases: ATP-dependent Clp protease ATP-binding subunit clpX-like, mitochondrial; caseinolytic peptidase X; caseinolytic protease X; ClpX caseinolytic peptidase X homolog; ClpX caseinolytic protease X homolog; energy-dependent regulator of proteolysis

Gene Aliases: AU014732; CLPX; E330029I21

UniProt ID: (Human) O76031, (Mouse) Q9JHS4, (Rat) Q5U2U0

Entrez Gene ID: (Human) 10845, (Mouse) 270166, (Rat) 300786

Function(s)

ATP-dependent peptidase activity protein binding ATP binding peptidase activator activity ATPase activity metal ion binding unfolded protein binding nucleotide binding molecular_function protease

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CLPX Polyclonal Antibody

CLPX Antibody (PA5-79052) in WB

Product Details

PA5-79052

Western Blot (WB)

Immunohistochemistry (Paraffin) (IHC (P))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

ELISA (ELISA)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Function(s)

Process(es)

Performance Guarantee

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