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Invitrogen
CNOT3 Polyclonal Antibody
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Application
FIGURE: 1 / 3
CNOT3 Antibody (PA5-100231) in ICC/IF
Immunofluorescent analysis of CNOT3 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CNOT3 polyclonal antibody (Product # PA5-100231) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).
Product Details
PA5-100231
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthesized peptide derived from human CNOT3(Accession O75175), corresponding to amino acid residues S258-V308.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.4, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2815761
Product Specific Information
Antibody detects endogenous levels of total CNOT3.
Target Information
Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity. [UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CCR4-associated factor 3; CCR4-NOT transcription complex subunit 3; CCR4-NOT transcription complex, subunit 3; Leukocyte receptor cluster member 2; NOT3 (negative regulator of transcription 3, yeast) homolog
Gene Aliases: A930039N10Rik; CNOT3; KIAA0691; LENG2; NOT3; NOT3H
UniProt ID: (Human) O75175, (Mouse) Q8K0V4
Entrez Gene ID: (Human) 4849, (Mouse) 232791, (Rat) 308311
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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