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CNTF Antibody (MA5-23730) in Neu
Neutralization assay of recombinant Rat CNTF stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by recombinant Rat CNTF (200 ng/mL) is neutralized (green line) by increasing concentrations of mouse Anti-Rat CNTF Monoclonal Antibody (Product # MA5-23730). The ND50 is typically 1-3 µg/mL.
Product Details
MA5-23730
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
34738
Immunogen
E. coli-derived recombinant rat CNTF Ala2-Met200
Conjugate
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609246
Product Specific Information
In sandwich ELISAs, less than 3% cross-reactivity with recombinant human CNTF is observed.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <1.0 EU per 1 µg of the antibody by the LAL method.
Target Information
The protein encoded by this gene is a polypeptide hormone whose actions appear to be restricted to the nervous system where it promotes neurotransmitter synthesis and neurite outgrowth in certain neuronal populations. The protein is a potent survival factor for neurons and oligodendrocytes and may be relevant in reducing tissue destruction during inflammatory attacks. A mutation in this gene, which results in aberrant splicing, leads to ciliary neurotrophic factor deficiency, but this phenotype is not causally related to neurologic disease. A read-through transcript variant composed of ZFP91 and CNTF sequence has been identified, but it is thought to be non-coding. Read-through transcription of ZFP91 and CNTF has also been observed in mouse.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ciliary neurotrophic factor; ciliary neurotropic factor; CNTF; H-CNTF
Gene Aliases: Cntf
UniProt ID: (Rat) P20294
Entrez Gene ID: (Rat) 25707
JAK-STAT cascade
nervous system development
positive regulation of cell proliferation
response to light intensity
positive regulation of phosphatidylinositol 3-kinase signaling
positive regulation of cell growth
growth
positive regulation of tyrosine phosphorylation of Stat3 protein
positive regulation of MAPK cascade
negative regulation of neuron apoptotic process
positive regulation of JAK-STAT cascade
negative regulation of photoreceptor cell differentiation
regulation of retinal cell programmed cell death
positive regulation of hormone secretion
astrocyte activation
muscle organ morphogenesis
neuron development
positive regulation of axon regeneration
protein heterooligomerization
response to electrical stimulus
regulation of resting membrane potential
membrane hyperpolarization
ciliary neurotrophic factor-mediated signaling pathway
negative regulation of neuron death
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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