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COL5A1 Antibody (PA5-109569) in WB
Western blot analysis of COL5A1 in Saos-2 cells. Samples were incubated with COL5A1 Polyclonal antibody (Product # PA5-109569) using a dilution of 1:3,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
Product Details
PA5-109569
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 300-400 of human COL5A1 (NP_0000843)
Conjugate
Form
Liquid
Concentration
1.77 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Wet ice
RRID
AB_2854980
Product Specific Information
Immunogen sequence: AKETTEVPEE LTPTPTEAAP MPETSEGAGK EEDVGIGDYD YVPSEDYYTP SPYDDLTYGE GEENPDQPTD PGAGAEIPTS TADTSNSSNP APPPGEGADD L
Target Information
Type I collagen, the fibrillar collagen found in most connective tissues, and the only component of the collagen found in cartilage. Mutations in this gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, and idiopathic osteoporosis. Reciprocal translocations between chromosomes 17 and 22, where this gene and the gene for platelet-derived growth factor beta are located, are associated with a particular type of skin tumor called dermatofibrosarcoma protuberans, resulting from unregulated expression of the growth factor. Two transcripts, resulting from the use of alternate polyadenylation signals, have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: alpha1(V) collagen; Collagen alpha-1(V) chain; collagen, type V, alpha 1; pro-alpha1(V) collagen; procollagen type V alpha 1; procollagen, type V, alpha 1
Gene Aliases: AI413331; COL5A1; EDSC
UniProt ID: (Human) P20908, (Mouse) O88207
Entrez Gene ID: (Human) 1289, (Mouse) 12831
blood vessel development
heart morphogenesis
cell adhesion
cell migration
extracellular matrix organization
collagen fibril organization
collagen catabolic process
collagen biosynthetic process
wound healing, spreading of epidermal cells
tendon development
extracellular fibril organization
skin development
integrin biosynthetic process
eye morphogenesis
regulation of cellular component organization
negative regulation of endodermal cell differentiation
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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