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Invitrogen
CRF Polyclonal Antibody
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Application
FIGURE: 1 / 1
CRF Antibody (PA5-116529) in WB
Western blot analysis of CRF in extracts of various cell lines. Samples were incubated with CRF Polyclonal antibody (Product # PA5-116529) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
PA5-116529
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 25-194 of human CRH (NP_0007471)
Conjugate
Unconjugated
Form
Liquid
Concentration
1.1 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2901160
Product Specific Information
Positive Samples: SH-SY5Y, Mouse liver, Mouse brain, Rat liver, Rat brain
Immunogen sequence: LLSRGPVPGA RQAPQHPQPL DFFQPPPQSE QPQQPQARPV LLRMGEEYFL RLGNLNKSPA APLSPASSLL AGGSGSRPSP EQATANFFRV LLQQLLLPRR SLDSPAALAE RGARNALGGH QEAPERERRS EEPPISLDLT FHLLREVLEM ARAEQLAQQA HSNRKLMEII
Target Information
Corticotropin Releasing Factor (CRF), a 41 amino acids neuropeptide, belongs to a family of structurally related peptides including urocortin, sauvagine and urotensin I. CRF is critical in the regulation of the pituitary-adrenal gland axis, and in endocrine, autonomic and behavioral responses to stress. CRF is widely distributed in the brain and peripheral nervous system with the highest levels in the hypothalamic paraventricular nucleus.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Corticoliberin; corticotropin releasing factor; Corticotropin-releasing factor; Corticotropin-releasing hormone; CRF
Gene Aliases: CRF; CRH; CRH1; Gm1347
UniProt ID: (Human) P06850, (Rat) P01143, (Mouse) Q8CIT0
Entrez Gene ID: (Human) 1392, (Rat) 81648, (Mouse) 12918
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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