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Application
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CRMP3 Antibody (MA5-42614) in WB
Western blot analysis of CRMP3 in extracts from C6 cells. Samples were incubated with CRMP3 Monoclonal antibody (Product # MA5-42614) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Product Details
MA5-42614
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
8N2S10
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human CRMP3/DPYSL4 (O14531).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.33 mg/mL
Purification
Affinity Chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol, 0.05% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2911755
Product Specific Information
Positive test controls include: C6.
Immunogen sequence: MSFQGKKSIP RITSDRLLIR GGRIVNDDQS FYADVHVEDG LIKQIGENLI VPGGIKTIDA HGLMVLPGGV DVHTRLQMPV LGMTPADDFC QGTKAALAGG
Target Information
Collapsin response mediator proteins (CRMPs), including CRMP-1 (DRP-1), CRMP-2 (DRP-2 or TOAD64), CRMP-3 (DRP-4), CRMP-4 (DRP-3) and CRMP-5 (DRP-5), mediate signal transduction after exposure of neural cells to the axon guidance molecule Semaphorin 3A (SEMA3A)/collapsin. CRMPs are present in the developing cerebral cortex and neocortical neurons and are responsive to SEMA3A. In the adult brain, the expression of CRMPs is dramatically downregulated. However, they remain expressed in structures that retain their capacity for differentiation and plasticity. CRMP-3 is expressed transiently in developing spinal cord and selectively in the postnatal cerebellum.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Collapsin response mediator protein 3; CRMP-3; Dihydropyrimidinase-related protein 4; DRP-4; ULIP-4; ULIP4 protein; UNC33-like phosphoprotein 4
Gene Aliases: Crmp3; Dpys4; Dpysl4; Ulip4
UniProt ID: (Rat) Q62951
Entrez Gene ID: (Rat) 25417
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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