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Invitrogen

CRY1 Polyclonal Antibody

1 Published Figure

2 References

View all (19) CRY1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (1)

Application

CRY1 Antibody in Immunocytochemistry (ICC/IF)

FIGURE: 1 / 2

CRY1 Antibody (PA1-527) in ICC/IF

Immunofluorescence analysis of Cryptochrome 1 was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cryptochrome 1 Rabbit Polyclonal Antibody (Product # PA1-527) at 2µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 con... View More

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Product Details

PA1-527

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000

Immunocytochemistry (ICC/IF)

2 µg/mL

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic Peptide: C Q(594) S V G P K V Q R Q S S N(606)

View immunogen

Conjugate

Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS with 1mg/mL BSA

Contains

0.05% sodium azide

Storage conditions

-20°C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_2276576

Product Specific Information

PA1-527 detects recombinant human cryptochrome 1 (Cry1).

PA1-527 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~70 kDa protein representing full length recombinant human Cry1 protein.

PA1-527 immunizing peptide corresponds to amino acid residues 594-606 from mouse Cry1. PA1-527 immunizing peptide (Cat. # PEP-103) is available for use in neutralization and control procedures.

Target Information

Various biochemical, physiological and behavioral processes display circadian rhythms controlled by an internal biological clock. The central "gears" driving this clock appear to be composed of an autoregulatory transcription/post translation-based feedback loop. Cryptochrome 1 (CRY1) and 2 (CRY2) are DNA-binding flavoproteins that bear some homology to blue-light receptors and photolyases. In Drosophila, CRY is a photoreceptor for the circadian clock where it binds to the clock component TIM in a light-dependent fashion and blocks its function. Mammalian CRY1 and CRY2 function via light-independent interactions with circadian genes CLOCK and BMAL1, as well as with PER1, PER2, and TIM. They seem to act as light-independent components of the circadian clock and likely regulate Per1 transcriptional cycling via interactions with both the activator and its feedback inhibitors. Mutant mice not expressing the Cry1 or Cry2 protein display accelerated and delayed periodicity of locomotor activity, respectively. It appears that the combination of both proteins working together is essential to synchronize the organism to circadian phases. A critical balance between Cry1 and Cry2 is required for proper clock function; in complete darkness, double-mutant mice present with instantaneous arrhythmicity, indicating the absence of an internal circadian clock.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Prolonged Administration of Melatonin Ameliorates Liver Phenotypes in Cholestatic Murine Model.

Cellular and molecular gastroenterology and hepatology

Ceci L,Chen L,Baiocchi L,Wu N,Kennedy L,Carpino G,Kyritsi K,Zhou T,Owen T,Kundu D,Sybenga A,Isidan A,Ekser B,Franchitto A,Onori P,Gaudio E,Mancinelli R,Francis H,Alpini G,Glaser S

Published figure using CRY1 polyclonal antibody (Product # PA1-527) in Immunocytochemistry

Mon Sep 26 00:00:00 UTC 2022

Melanopsin and rod-cone photoreceptive systems account for all major accessory visual functions in mice.

Nature

Hattar S,Lucas RJ,Mrosovsky N,Thompson S,Douglas RH,Hankins MW,Lem J,Biel M,Hofmann F,Foster RG,Yau KW

PA1-527 was used in immunocytochemistry to assay for Cry1 in eyes of the Opn4-/- Gnat1-/- Cnga3-/- mice

Thu Jul 03 00:00:00 UTC 2003

Bioinformatics

Protein Aliases: cryptochrome 1 (photolyase-like); Cryptochrome-1

Gene Aliases: AU020726; AU021000; CRY1; PHLL1

UniProt ID: (Human) Q16526, (Mouse) P97784

Entrez Gene ID: (Human) 1407, (Mouse) 12952

Function(s)

nucleotide binding transcription factor activity, transcription factor binding core promoter sequence-specific DNA binding core promoter binding DNA binding double-stranded DNA binding deoxyribodipyrimidine photo-lyase activity DNA (6-4) photolyase activity protein binding transcription factor binding blue light photoreceptor activity protein kinase binding phosphatase binding nuclear hormone receptor binding histone deacetylase binding ubiquitin binding DNA photolyase activity photoreceptor activity kinase binding DNA photolyase

Process(es)

negative regulation of transcription from RNA polymerase II promoter gluconeogenesis transcription, DNA-templated DNA damage induced protein phosphorylation blue light signaling pathway protein-chromophore linkage lipid storage negative regulation of protein ubiquitination response to insulin circadian regulation of gene expression response to glucagon glucose homeostasis regulation of circadian rhythm negative regulation of circadian rhythm entrainment of circadian clock by photoperiod negative regulation of G-protein coupled receptor protein signaling pathway negative regulation of transcription, DNA-templated regulation of DNA damage checkpoint negative regulation of glucocorticoid receptor signaling pathway negative regulation of glucocorticoid secretion DNA repair regulation of transcription, DNA-templated circadian rhythm rhythmic process response to stimulus

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