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Invitrogen
CXCL2 Monoclonal Antibody (40605)
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Application
FIGURE: 1 / 5
CXCL2 Antibody (MA5-23737) in ICC/IF
Immunofluorescence analysis of C-X-C motif chemokine 2 (CXCL2) was performed using 70% confluent log phase untreated and LPS treated (100 ng/mL for 7 hr) RAW 264.7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with CXCL2 Monoclonal Antibody (40605) (Product # MA5-23737) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) H... View More
Product Details
MA5-23737
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
40605
Immunogen
E. coli-derived recombinant mouse CXCL2/GRO beta/MIP-2/CINC-3 Ala28-Asn100
Conjugate
Unconjugated
Form
Lyophilized
Concentration
0.5 mg/mL
Purification
Protein A/G
Storage buffer
PBS with 5% trehalose
Contains
No Preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2609513
Product Specific Information
In ELISAs, does not cross-react with recombinant human (rh) CXCL2/GRO beta, rhCXCL3/GRO gamma, or recombinant mouse CXCL1/KC.
Reconstitute at 0.5 mg/mL in sterile PBS.
Endoxin level is <0.10 EU per 1 µg of the antibody by the LAL method.
Target Information
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C motif chemokine 2; CC motif chemokine; CCmotif chemokine; CXC; CXC motif chemokine; CXCL; GRO b; gro beta; GRO β; GRObeta; GROβ; Macrophage inflammatory protein 2; MIP 2 a; MIP 2 alpha; MIP 2 α; MIP2; MIP2 a; MIP2 α; small inducible cytokine subfamily, member 2
Gene Aliases: CINC-2a; Cxcl2; Gro2; GROb; Mgsa-b; MIP-2; MIP-2a; Mip2; Scyb; Scyb2
UniProt ID: (Mouse) P10889
Entrez Gene ID: (Mouse) 20310
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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