Invitrogen

CXCR4 Polyclonal Antibody

CXCR4 Antibody in Immunocytochemistry (ICC/IF)

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CXCR4 Antibody (PA3-305) in ICC/IF

Immunofluorescent analysis of CXCR4 (green) showing staining in the cytoplasm of U251 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CXCR4 polyclonal antibody (Product # PA3-305) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4ºC in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugate... View More

CXCR4 Antibody in Immunohistochemistry (IHC)

CXCR4 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Product Details

PA3-305

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000-1:5,000

View 2 publications 2 publications

Immunohistochemistry (IHC)

View 10 publications 10 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:1,000-1:5,000

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

1:1,000-1:5,000

View 3 publications 3 publications

Flow Cytometry (Flow)

1:1,000-1:5,000

Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Mouse

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Synthetic peptide corresponding to residues K(338) G K R G G H S S V S T E S E S S S F H S S (359) of human CXCR4 receptor protein.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

whole serum

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2091817

Product Specific Information

Several different molecular weight isoforms of CXCR4 have been described in the literature, which vary depending on cell type tested and extraction buffer used. Using Ramos, Raji, and Jurkat membrane fractions, two different MW forms of CXCR4 were detected with PA3-305 by Western blot, between 55-72kD.

It is recommended to use a membrane isolation buffer/kit for extracting CXCR4 for Western blot analysis. Cellular membrane fractions were isolated for Western blot using Mem-PER Plus (Product # 89842).

Target Information

CXCR4 (Fusin, LESTR, HUMSTR) is a member of the G-protein-coupled chemokine receptor family with seven membrane-spanning domains, and functions as a co-receptor for X4 HIV-1 entry into CD4+ cells. CXCR4 has been proved to be the co-receptor for HIV-2's binding to CD4 through envelope glycoprotein gp 120 and promotes Env-mediated fusion of the virus. In other cases, CXCR4 can even function as the only receptor for HIV-2's binding to the CD4 host cells. CXCR4 is the receptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ion levels and enhancing MAPK1/MAPK3 activation. CXCR4 acts as a receptor for extracellular ubiquitin; leading to enhanced intracellular calcium ions and reduced cellular cAMP levels. Also, CXCR4 is involved in hematopoiesis and in cardiac ventricular septum formation, and plays an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. In the CNS, CXCR4 could mediate hippocampal-neuron survival and is involved in cerebellar development. CXCR4 binds bacterial lipopolysaccharide (LPS) and mediates LPS-induced inflammatory response, including TNF secretion by monocytes. Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells. The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Myometrial-derived CXCL12 promotes lipopolysaccharide induced preterm labour by regulating macrophage migration, polarization and function in mice.

Journal of cellular and molecular medicine

Zhang L,Mamillapalli R,Habata S,McAdow M,Taylor HS

PA3-305 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to show that smooth muscle cell treatment for pre-term labour (PTL) in mice with AMD3100 reduces lipopolysaccharide-induced PTL through blocking of CXCL12/CXCR4 signalling.

Sun May 01 00:00:00 EDT 2022

Lidocaine inhibited migration of NSCLCA549 cells via the CXCR4 regulation.

Cancer biomarkers : section A of Disease markers

Xing B,Yang L,Cui Y

PA3-305 was used in Western Blotting to show that Lidocaine at clinical plasma concentrations inhibited CXCL12-induced CXCR4 activation, thereby reduced the intracellular Ca2+-dependent cytoskeleton remodeling, resulting in slower migration of A549 cells.

Tue Apr 19 00:00:00 EDT 2022

Enhanced anti-inflammatory effects of mesenchymal stromal cells mediated by the transient ectopic expression of CXCR4 and IL10.

Stem cell research & therapy

Hervás-Salcedo R,Fernández-García M,Hernando-Rodríguez M,Quintana-Bustamante O,Segovia JC,Alvarez-Silva M,García-Arranz M,Minguez P,Del Pozo V,de Alba MR,García-Olmo D,Ayuso C,Lamana ML,Bueren JA,Yañez RM

PA3-305 was used in Immunocytochemistry to show that the transient co-expression of CXCR4 and IL10 enhances the therapeutic potential of mesenchymal stromal cells in a local inflammation mouse model.

Fri Feb 12 00:00:00 EST 2021

Enhanced anti-inflammatory effects of mesenchymal stromal cells mediated by the transient ectopic expression of CXCR4 and IL10.

Stem cell research & therapy

PA3-305 was used in Immunocytochemistry to show that the transient co-expression of CXCR4 and IL10 enhances the therapeutic potential of mesenchymal stromal cells in a local inflammation mouse model.

Fri Feb 12 00:00:00 EST 2021

CXCL12 enhances pregnancy outcome via improvement of endometrial receptivity in mice.

Scientific reports

Koo HS,Yoon MJ,Hong SH,Ahn J,Cha H,Lee D,Ko JE,Kwon H,Choi DH,Lee KA,Ko JJ,Kang YJ

PA3-305 was used in Immunohistochemistry to develop a minimally-invasive therapeutic strategy for clinical treatment or supplement for the patients with repeated implantation failure with less risk.

Thu Apr 01 00:00:00 EDT 2021

CXCL12 enhances pregnancy outcome via improvement of endometrial receptivity in mice.

Scientific reports

Koo HS,Yoon MJ,Hong SH,Ahn J,Cha H,Lee D,Ko JE,Kwon H,Choi DH,Lee KA,Ko JJ,Kang YJ

PA3-305 was used in Immunohistochemistry to develop a minimally-invasive therapeutic strategy for clinical treatment or supplement for the patients with repeated implantation failure with less risk.

Thu Apr 01 00:00:00 EDT 2021

Clinical and Biological Characteristics of Medullary and Extramedullary Plasma Cell Dyscrasias.

Biology

Janjetovic S,Lohneis P,Nogai A,Balci D,Rasche L,Jähne D,Bokemeyer C,Schilling G,Blau IW,Schmidt-Hieber M

PA3-305 was used in Immunohistochemistry to obtain insights into the molecular mechanisms of extramedullary spread of clonal plasma cells.

Tue Jul 06 00:00:00 EDT 2021

Loss of Cxcr4 in Endometriosis Reduces Proliferation and Lesion Number while Increasing Intraepithelial Lymphocyte Infiltration.

The American journal of pathology

Tal A,Tal R,Kliman HJ,Taylor HS

PA3-305 was used in Immunohistochemistry-immunofluorescence to explore the effects of targeted disruption of CXCR4 in endometriosis lesions.

Thu Jul 01 00:00:00 EDT 2021

CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.

Frontiers in cell and developmental biology

Verheijen N,Suttorp CM,van Rheden REM,Regan RF,Helmich MPAC,Kuijpers-Jagtman AM,Wagener FADTG

Published figure using CXCR4 polyclonal antibody (Product # PA3-305) in Immunohistochemistry

Mon Sep 28 00:00:00 EDT 2020

CXCL12-CXCR4 Interplay Facilitates Palatal Osteogenesis in Mice.

Frontiers in cell and developmental biology

Verheijen N,Suttorp CM,van Rheden REM,Regan RF,Helmich MPAC,Kuijpers-Jagtman AM,Wagener FADTG

Published figure using CXCR4 polyclonal antibody (Product # PA3-305) in Immunohistochemistry

Mon Sep 28 00:00:00 EDT 2020

An injectable hydrogel-formulated inhibitor of prolyl-4-hydroxylase promotes T regulatory cell recruitment and enhances alveolar bone regeneration during resolution of experimental periodontitis.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Nagai K,Ideguchi H,Kajikawa T,Li X,Chavakis T,Cheng J,Messersmith PB,Heber-Katz E,Hajishengallis G

PA3-305 was used in Immunohistochemistry to show that the administration of 1,4-DPCA/hydrogel at the onset of periodontitis resolution promotes CXCR4-dependent accumulation of Treg cells and alveolar bone regeneration, suggesting a novel approach for regaining bone lost due to periodontitis.

Thu Oct 01 00:00:00 EDT 2020

CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking.

Journal of cellular and molecular medicine

Pluchino N,Mamillapalli R,Shaikh S,Habata S,Tal A,Gaye M,Taylor HS

PA3-305 was used in Immunohistochemistry (Paraffin) to propose that CXCR4 and CXCR7 antagonists can be used as potential novel, non-hormonal therapies for endometriosis.

Sat Feb 01 00:00:00 EST 2020

CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking.

Journal of cellular and molecular medicine

Pluchino N,Mamillapalli R,Shaikh S,Habata S,Tal A,Gaye M,Taylor HS

PA3-305 was used in Immunohistochemistry (Paraffin) to propose that CXCR4 and CXCR7 antagonists can be used as potential novel, non-hormonal therapies for endometriosis.

Sat Feb 01 00:00:00 EST 2020

CXCR4 or CXCR7 antagonists treat endometriosis by reducing bone marrow cell trafficking.

Journal of cellular and molecular medicine

Pluchino N,Mamillapalli R,Shaikh S,Habata S,Tal A,Gaye M,Taylor HS

PA3-305 was used in Immunohistochemistry (Paraffin) to propose that CXCR4 and CXCR7 antagonists can be used as potential novel, non-hormonal therapies for endometriosis.

Sat Feb 01 00:00:00 EST 2020

Oral lichen planus interactome reveals CXCR4 and CXCL12 as candidate therapeutic targets.

Scientific reports

Rivera C,Crisóstomo MF,Peña C,González-Díaz P,González-Arriagada WA

PA3-305 was used in Immunohistochemistry to support the pathological role of chemokines and chemokine receptors in oral lichen planus.

Wed Mar 25 00:00:00 EDT 2020

Oral lichen planus interactome reveals CXCR4 and CXCL12 as candidate therapeutic targets.

Scientific reports

Rivera C,Crisóstomo MF,Peña C,González-Díaz P,González-Arriagada WA

PA3-305 was used in Immunohistochemistry to support the pathological role of chemokines and chemokine receptors in oral lichen planus.

Wed Mar 25 00:00:00 EDT 2020

Prognostic value of immunoexpression of CCR4, CCR5, CCR7 and CXCR4 in squamous cell carcinoma of tongue and floor of the mouth.

Medicina oral, patologia oral y cirugia bucal

Domingueti CB,Janini JB,Paranaíba LM,Lozano-Burgos C,Olivero P,González-Arriagada WA

PA3-305 was used in Immunohistochemistry to evaluate the prognostic value of the immunoexpression of chemokines in squamous cell carcinoma of tongue and floor of the mouth.

Wed May 01 00:00:00 EDT 2019

Prognostic value of immunoexpression of CCR4, CCR5, CCR7 and CXCR4 in squamous cell carcinoma of tongue and floor of the mouth.

Medicina oral, patologia oral y cirugia bucal

Domingueti CB,Janini JB,Paranaíba LM,Lozano-Burgos C,Olivero P,González-Arriagada WA

PA3-305 was used in Immunohistochemistry to evaluate the prognostic value of the immunoexpression of chemokines in squamous cell carcinoma of tongue and floor of the mouth.

Wed May 01 00:00:00 EDT 2019

Fibrocytes in Chronic Periaortitis: A Novel Mechanism Linking Inflammation and Fibrosis.

Arthritis & rheumatology (Hoboken, N.J.)

Nicastro M,Vescovini R,Maritati F,Palmisano A,Urban ML,Incerti M,Fenaroli P,Peyronel F,Benigno GD,Mangieri D,Volpi R,Becchi G,Romagnani P,Corradi D,Vaglio A

PA3-305 was used in Flow Cytometry, Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to indicate that fibrocytes are enriched in the peripheral blood of CP patients and infiltrate target lesions and that the accumulation of fibrocytes in the pathologic tissue might be driven by CXCL12, and Th2-skewed immune responses are likely to facilitate their differentiation.

Fri Nov 01 00:00:00 EDT 2019

CXCR4 receptor blockage reduces the contribution of tumor and stromal cells to the metastatic growth in the liver.

Oncology reports

Benedicto A,Romayor I,Arteta B

PA3-305 was used in Western Blotting to investigate the effectiveness of CXCR4 receptor blockage, and its mechanisms, in reducing metastatic growth in the liver.

Sun Apr 01 00:00:00 EDT 2018

Locoregional Confinement and Major Clinical Benefit of ¹⁸⁸Re-Loaded CXCR4-Targeted Nanocarriers in an Orthotopic Human to Mouse Model of Glioblastoma.

Theranostics

Séhédic D,Chourpa I,Tétaud C,Griveau A,Loussouarn C,Avril S,Legendre C,Lepareur N,Wion D,Hindré F,Davodeau F,Garcion E

PA3-305 was used in Immunohistochemistry to investigate the efficacy of a novel system of local brain internal radiation therapy, combining a radiopharmaceutical with an immuno-nanoparticle.

Mon Mar 19 00:00:00 EDT 2018

CXCR4 regulates interneuron migration in the developing neocortex.

The Journal of neuroscience : the official journal of the Society for Neuroscience

Stumm RK,Zhou C,Ara T,Lazarini F,Dubois-Dalcq M,Nagasawa T,Höllt V,Schulz S

PA3-305 was used in immunohistochemistry to investigate the important role of CXCR4 in interneuron migration

Sun Jun 15 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: C Cmotif chemokine; C X C motif chemokine; C-X-C chemokine receptor type 4; CC motif chemokine; CCmotif chemokine; CD antigen CD184; CD184; CD184 antigen; chemokine (C-X-C motif) receptor 4; chemokine (C-X-C) receptor 4; chemokine receptor; chemokine receptor 4; CXC; CXC motif chemokine; CXC-R4; CXCR-4; FB22; Fusin; HM89; HUMSTR; LAP-3; LCR1; LESTR; Leukocyte-derived seven transmembrane domain receptor; leukocyte-expressed seven-transmembrane-domain; Lipopolysaccharide-associated protein 3; LPS-associated protein 3; neuropeptide Y receptor Y3; neuropeptide Y3 receptor; NPYRL; PB-CKR; Pre-B-cell-derived chemokine receptor; SDF-1 receptor; seven transmembrane helix receptor; seven-transmembrane-segment receptor, spleen; Stromal cell-derived factor 1 receptor

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Gene Aliases: b2b220Clo; CD184; Cmkar4; CXCR4; D2S201E; FB22; HM89; HSY3RR; LAP-3; LAP3; LCR1; LESTR; NPY3R; NPYR; NPYRL; NPYY3R; PB-CKR; PBSF/SDF-1; Sdf1r; WHIM; WHIMS

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UniProt ID: (Human) P61073, (Mouse) P70658

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Entrez Gene ID: (Human) 7852, (Mouse) 12767

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Function(s)

virus receptor activity actin binding G-protein coupled receptor activity protein binding drug binding coreceptor activity C-X-C chemokine receptor activity cytokine binding ubiquitin protein ligase binding myosin light chain binding ubiquitin binding signal transducer activity chemokine receptor activity

Process(es)

patterning of blood vessels ameboidal-type cell migration neuron migration epithelial cell development chemotaxis signal transduction G-protein coupled receptor signaling pathway germ cell development nervous system development brain development motor neuron axon guidance germ cell migration organ morphogenesis cell migration calcium-mediated signaling telencephalon cell migration regulation of cell migration T cell proliferation neutrophil activation regulation of programmed cell death myelin maintenance positive regulation of oligodendrocyte differentiation regulation of chemotaxis regulation of calcium ion transport cardiac muscle contraction chemokine-mediated signaling pathway cellular response to cytokine stimulus activation of MAPK activity response to hypoxia dendritic cell chemotaxis apoptotic process inflammatory response positive regulation of cytosolic calcium ion concentration neuron recognition response to virus fusion of virus membrane with host plasma membrane entry into host cell

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CXCR4 Polyclonal Antibody

CXCR4 Antibody (PA3-305) in ICC/IF

Product Details

PA3-305

Western Blot (WB)

Immunohistochemistry (IHC)

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Immunogen

Conjugate

Form

Concentration

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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