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Application
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CYB5R3 Antibody (PA5-19196) in WB
Western blot analysis of CYB5R3 using CYB5R3 Polyclonal Antibody (Product # PA5-19196) (0.1 µg/mL) in staining of Mouse Liver lysate (RIPA buffer, 35 µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Product Details
PA5-19196
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Goat
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide sequence (PNLERVGHPKERC) corresponding to the C-terminus amino acids of Cyb5r3 (aa 286-299).
Conjugate
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Purification
Ammonium sulfate precipitation
Storage buffer
TBS, pH 7.3, with 0.5% BSA
Contains
0.02% sodium azide
Storage conditions
-20° C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_10982756
Product Specific Information
This antibody is predicted to react with bovine, canine and rat based on sequence homology.
This antibody is tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
CYB5R3 (NADH-cytochrome b5, reductase 3, diaphorase 1) is a 301 amino acid protein encoded by the human gene CYB5R3. CYB5R3 belongs to the flavoprotein pyridine nucleotide cytochrome reductase family and has two naturally occuring isoforms. Isoform 1 is anchored to the cytoplasmic side of the endoplasmic reticulum membrane and mitochondrion outer membrane, while isoform 2 is the soluble form found in erythrocytes. CYB5R3 is involved in the desaturation and elongation of fatty acids, cholesterol biosynthesis,drug metabolism and, in erythrocytes, methemoglobin reduction.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: B5R; diaphorase 1 (NADH); Diaphorase-1; Diaphorase1; NADH-cytochrome b5 reductase 3
Gene Aliases: 0610016L08Rik; 2500002N19Rik; C85115; Cyb5r3; Dia-1; Dia1; WU:AL591952.1-001; WU:AL591952.1-002; WU:AL591952.1-003; WU:Cyb5r3
UniProt ID: (Mouse) Q9DCN2
Entrez Gene ID: (Mouse) 109754
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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