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Application
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Cytochrome C Antibody (A305-281A-T) in WB
Detection of human and mouse Cytochrome C by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, Jurkat, and mouse TCMK-1 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Cytochrome C antibody (Product # A305-281A) (lot A305-281A-1) used for WB at 0.1 µg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
Product Details
A305-281A-T
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Region between residue 55 to 105 of human Cytochrome C.
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
phosphate/tris citrate, pH 7-8
Contains
0.09% sodium azide
Storage conditions
4° C
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Rat, Sheep, Bovine, Horse, Rabbit, Pig, Orangutan, Monkey, Gorilla and Chimpanzee
Target Information
Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in oxidative phosphorylation and the production of cellular ATP. Release of cytochrome C from the mitochondria to the cytosol is triggered by apoptotic stimuli. In the cytoplasm, the cytochrome C binds apoptotic protease activating factor which activates the apoptotic initiator procaspase 9. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CRF; Cytochrome c; Cytochrome c, somatic; hc1
Gene Aliases: CYC; CYCS; HCS; THC4
UniProt ID: (Human) P99999, (Mouse) P62897
Entrez Gene ID: (Human) 54205, (Mouse) 13063
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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